NAE1 Antibody

說明書

中文名稱：

NAE1兔多克隆抗體
貨號：

CSB-PA195686
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA195686(NAE1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA195686(NAE1 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q13564
基因名：

NAE1
別名：

59 kDa antibody; A 116A10.1 antibody; Amyloid beta precursor protein binding protein 1 59kD antibody; Amyloid beta precursor protein binding protein 1 59kDa antibody; Amyloid beta precursor protein binding protein 1 antibody; Amyloid beta precursor protein binding protein 1, 59 kDa antibody; Amyloid beta precursor protein-binding protein 1 antibody; Amyloid protein binding protein 1 antibody; Amyloid protein-binding protein 1 antibody; APP BP1 antibody; APP-BP1 antibody; APPBP 1 antibody; APPBP1 antibody; HPP 1 antibody; HPP1 antibody; NAE 1 antibody; NAE1 antibody; NEDD8 activating enzyme E1 regulatory subunit antibody; NEDD8 activating enzyme E1 subunit 1 antibody; NEDD8 activating enzyme E1 subunit antibody; NEDD8-activating enzyme E1 regulatory subunit antibody; Proto oncogene protein 1 antibody; Proto-oncogene protein 1 antibody; Protooncogene protein 1 antibody; ula 1 antibody; ULA1_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Fusion protein of Human NAE1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:2000
IHC	1:15-1:50

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
基因功能參考文獻：
1. Here we report that high expression of neddylation components, NEDD8 and NAE1, are associated with poor survival of Pancreatic ductal adenocarcinoma patients. Blockage of neddylation by MLN4924 significantly sensitizes pancreatic cancer cells to gemcitabine, reduced clonogenic survival, decreased invasion capacity, increased apoptosis and senescence PMID: 28535453
2. E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma PMID: 25229838
3. analysis of a metal-based inhibitor of NEDD8-activating enzyme PMID: 23185368
4. our results suggest that APP-BP1 modulates neuronal differentiation by altering gene expression profiles in fetal neural stem cells PMID: 22182960
5. APP-BP1 plays an important role in the cell cycle progression of fetal neural stem cells PMID: 21151996
6. APP-BP1 displayed decreased expression levels in HPV-positive head and neck squamous cell carcinoma PMID: 19795456
7. structure and mutational analysis of human APPBP1-UBA3, the heterodimeric E1 enzyme for NEDD8 PMID: 12646924
8. Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer. PMID: 12740388
9. Data report the structure of the quaternary complex between human APPBP1-UBA3, a heterodimeric E1, its ubl NEDD8, and ATP. PMID: 14690597
10. APP-BP1 is degraded by ubiquitin-dependent proteolysis. PMID: 18627766
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亞細胞定位：

Cell membrane. Note=Colocalizes with APP in lipid rafts.
蛋白家族：

Ubiquitin-activating E1 family, ULA1 subfamily
組織特異性：

Ubiquitous in fetal tissues. Expressed throughout the adult brain.
數據庫鏈接：

HGNC: 621

OMIM: 603385

KEGG: hsa:8883

STRING: 9606.ENSP00000290810

UniGene: Hs.460978

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