MOB1B Antibody

中文名稱：

MOB1B兔多克隆抗體
貨號：

CSB-PA762574HA01HU
規格：

￥440
圖片：
IHC image of CSB-PA762574HA01HU diluted at 1:1200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA762574HA01HU diluted at 1:1200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of NIH/3T3 cells with CSB-PA762574HA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cells with CSB-PA762574HA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating MOB1B in K562 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA762574HA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA762574HA01HU (6µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (10µg)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) MOB1B Polyclonal antibody
Uniprot No.：

Q7L9L4
基因名：

MOB1B
別名：

MATS 2 antibody; MATS2 antibody; MGC33910 antibody; Mob 1A antibody; Mob 1B antibody; MOB 4A antibody; MOB kinase activator 1B antibody; Mob1 homolog 1A antibody; MOB1 Mps One Binder homolog B antibody; MOB1 Mps one binder kinase activator like 1A antibody; Mob1A antibody; Mob1B antibody; MOBKL 1A antibody; MOBKL1A antibody; MOL1A_HUMAN antibody; Mps one binder kinase activator like 1A antibody; Mps one binder kinase activator-like 1A antibody; Protein Mob4A antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human MOB kinase activator 1B protein (2-216AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，MOB1B Antibody (CSB-PA762574HA01HU)，的標記方式是Non-conjugated。對于MOB1B Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA762574HB01HU	MOB1B Antibody, HRP conjugated	ELISA
FITC	CSB-PA762574HC01HU	MOB1B Antibody, FITC conjugated
Biotin	CSB-PA762574HD01HU	MOB1B Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:2000
IF	1:200-1:500
IP	1:200-1:2000

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.
亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

MOB1/phocein family
組織特異性：

Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver.
數據庫鏈接：

HGNC: 29801

OMIM: 609282

KEGG: hsa:92597

STRING: 9606.ENSP00000310189

UniGene: Hs.691454

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