METAP2 Antibody

中文名稱：

METAP2兔多克隆抗體
貨號：

CSB-PA013716LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Rat liver tissue
All lanes: METAP2 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 52, 51 kDa
Observed band size: 53 kDa

IHC image of CSB-PA013716LA01HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA013716LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) METAP2 Polyclonal antibody
Uniprot No.：

P50579
基因名：

METAP2
別名：

A930035J23Rik antibody; AI047573 antibody; AL024412 antibody; Amp2 antibody; AU014659; antibody; EIF 2 associated p67 homolog antibody; EIF-2-associated p67 antibody; Initiation factor 2 associated 67 kDa glycoprotein antibody; Initiation factor 2-associated 67 kDa glycoprotein antibody; MAP 2 antibody; MAP2 antibody; MAP2_HUMAN antibody; MetAP 2 antibody; Metap2 antibody; Methionine aminopeptidase 2 antibody; Methionyl aminopeptidase 2 antibody; MGC102452 antibody; MGC127390 antibody; MGC53792 antibody; MNPEP antibody; p67 antibody; p67eIF2 antibody; Peptidase M antibody; Peptidase M2 antibody
宿主：

Rabbit
反應種屬：

Human, Rat
免疫原：

Recombinant Human Methionine aminopeptidase 2 protein (313-449AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，METAP2 Antibody (CSB-PA013716LA01HU)，的標記方式是Non-conjugated。對于METAP2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA013716LB01HU	METAP2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA013716LC01HU	METAP2 Antibody, FITC conjugated
Biotin	CSB-PA013716LD01HU	METAP2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
基因功能參考文獻：
1. MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
2. Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
3. Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
4. Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
5. The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
6. Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
7. This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
8. the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
9. Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
10. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
11. High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
12. Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
13. MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
14. Expressed in colon cancer cells PMID: 15336565
15. MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
16. A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
17. Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
18. MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
19. Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
20. Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176
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亞細胞定位：

Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
蛋白家族：

Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
數據庫鏈接：

HGNC: 16672

OMIM: 601870

KEGG: hsa:10988

STRING: 9606.ENSP00000325312

UniGene: Hs.444986

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