METAP1 Antibody
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中文名稱：METAP1兔多克隆抗體
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貨號(hào)：CSB-PA013715LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Rat brain tissue
All lanes: METAP1 antibody at 6.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa -
IHC image of CSB-PA013715LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA013715LA01HU diluted at 1:400 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA013715LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) METAP1 Polyclonal antibody
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Uniprot No.：
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基因名：METAP1
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別名：AMPM1_HUMAN antibody; DKFZp781C0419 antibody; KIAA0094 antibody; MAP 1 antibody; MAP1A antibody; MetAP 1 antibody; metap1 antibody; MetAP1A antibody; Methionine aminopeptidase 1 antibody; Peptidase M 1 antibody
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宿主：Rabbit
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反應(yīng)種屬：Human, Rat
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免疫原：Recombinant Human Methionine aminopeptidase 1 protein (54-125AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁(yè)面中的產(chǎn)品，METAP1 Antibody (CSB-PA013715LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于METAP1 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
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基因功能參考文獻(xiàn)：
- Data indicate that pyridinylpyrimidine-based molecules displayed species specificity behavior against methionine aminopeptidases (MetAPs). PMID: 23767698
- Data show that human methionine aminopeptidase 1 (MetAP1) fully rescued the slow growth phenotype associated with deletion of yeast MetAP1, suggesting that the yeast and human proteins may have similar roles in vivo. PMID: 12144506
- results suggest that MetAP1 plays an important role in G(2)/M phase of the cell cycle and that it may serve as a promising target for the discovery and development of new anticancer agents PMID: 17114291
- Human MetAP1 is distinct from other members of the MetAP superfamily in the number of metal ions employed and likely mechanism of catalysis. PMID: 17929833
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亞細(xì)胞定位：Cytoplasm.
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蛋白家族：Peptidase M24A family, Methionine aminopeptidase type 1 subfamily
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數(shù)據(jù)庫(kù)鏈接：
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