LEO1 Antibody

中文名稱：

LEO1兔多克隆抗體
貨號：

CSB-PA845150NA01HU
規格：

￥440
圖片：
IHC image of CSB-PA845150NA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: LEO1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 76, 69 kDa
Observed band size: 76 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) LEO1 Polyclonal antibody
Uniprot No.：

Q8WVC0
基因名：

LEO1
別名：

Gm185 antibody; LEO1 antibody; LEO1_HUMAN antibody; LOC123439 antibody; Paf1/RNA polymerase II complex component antibody; Replicative senescence down regulated leo1 like protein antibody; Replicative senescence down-regulated leo1-like protein antibody; Replicative senescence downregulated leo1 like antibody; RNA polymerase associated protein LEO1 antibody; RNA polymerase-associated protein LEO1 antibody; Senescence downregulated leo1 like antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Peptide sequence from Human RNA polymerase-associated protein LEO1 protein (2-24AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，LEO1 Antibody (CSB-PA845150NA01HU)，的標記方式是Non-conjugated。對于LEO1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA845150NB01HU	LEO1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA845150NC01HU	LEO1 Antibody, FITC conjugated
Biotin	CSB-PA845150ND01HU	LEO1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling.
基因功能參考文獻：
1. PRL-3 and Leo1 levels were positively associated in AML patient samples. PMID: 24686170
2. Screened glioblastomas and oligodendrogliomas for fusion genes by identifying aberrant 5'-3' expression of genes that lie over regions of a copy number change. A fusion gene between exon 11 of LEO1 and exon 10 of SLC12A1 was identified. PMID: 20196086
亞細胞定位：

Nucleus.
蛋白家族：

LEO1 family
組織特異性：

Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver.
數據庫鏈接：

HGNC: 30401

OMIM: 610507

KEGG: hsa:123169

STRING: 9606.ENSP00000299601

UniGene: Hs.567662

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