LCT Antibody

中文名稱：

LCT兔多克隆抗體
貨號：

CSB-PA012827LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA012827LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
其他：

產品詳情
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靶點詳情

產品詳情

產品描述：

The LCT antibody can be used to recognize the human LCT protein. A rabbit got immunization with the recombinant human LCT protein (983-1252aa). After that, an immune response occurred in the rabbit producing large amounts of antibodies against the LCT protein. The rabbit's blood was collected and isolated to get the anti-serum, which was protein G-purified to obtain the LCT polyclonal antibody (purity 95%⁺). This LCT antibody has accessed its specificity in ELISA and IHC applications.

The LCT is an enzyme that breaks down lactose into glucose and galactose in the small intestine. Therefore, the main function of LCT protein is the digestion of lactose, a sugar found in milk and dairy products, in order to provide a source of energy and nutrients to the body. Deficiency in lactase activity can result in lactose intolerance, which can cause digestive symptoms such as bloating, gas, and diarrhea.
產品名稱：

Rabbit anti-Homo sapiens (Human) LCT Polyclonal antibody
Uniprot No.：

P09848
基因名：

LCT
別名：

LAC antibody; Lactase antibody; Lactase phlorizin hydrolase 1 antibody; Lactase phlorizin hydrolase antibody; Lactase-glycosylceramidase antibody; Lct antibody; LPH antibody; LPH_HUMAN antibody; LPH1 antibody; Phlorizin hydrolase antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Lactase-phlorizin hydrolase protein (983-1252AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，LCT Antibody (CSB-PA012827LA01HU)，的標記方式是Non-conjugated。對于LCT Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA012827LB01HU	LCT Antibody, HRP conjugated	ELISA
FITC	CSB-PA012827LC01HU	LCT Antibody, FITC conjugated
Biotin	CSB-PA012827LD01HU	LCT Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

LPH splits lactose in the small intestine.
基因功能參考文獻：
1. Did not find any observational or genetic association between milk intake and acne using the lactase persistent/non-persistent LCT-13910 C/T genotype in a Mendelian randomization design. PMID: 30096803
2. Large-scale studies with different ethnic populations will be needed to further investigate the possible race-specific effect of LCT 13910 C/T polymorphism on bone mineral density and fracture risk PMID: 29321359
3. Novel genetic variants have been identified in the mutational hotspot region 14 kb upstream of the lactase gene in a rural Mexican population. PMID: 28452238
4. In the Portuguese sample of young adults, the lactase -13910C>T polymorphism revealed significant associations with the obesity-related anthropometric variables body mass index, fat mass and weight, and previously observed associations with the obesity risk were also confirmed. PMID: 27577176
5. Lactase Persistence in Humans and its evolution is reviewed. PMID: 28426286
6. In Caucasians, the CC genotype that predicts lactose intolerance is associated with a lower plasma 25(OH)D concentration, which is attributable at least in part to a lower intake of dairy, particularly skim milk. Increased risk of suboptimal concentrations of vitamin D was also observed among those with the CT genotype, suggesting an intermediate effect of the heterozygous genotype. PMID: 28446633
7. Three allelic variants alter promoter function and upregulate LCT gene expression. PMID: 27714771
8. Genetic factors contribute to epigenetic changes occurring with age at the regulatory elements, because lactase-persistence and lactase-nonpersistence DNA haplotypes demonstrated markedly different epigenetic aging. PMID: 27159559
9. Combination of real-time PCR and sequencing was used to detect multiple clinically relevant genetic variations in the lactase gene in Danish patients with lactose intolerance. PMID: 27937006
10. The frequency of -13910*T was significantly higher among the Mennonites when compared to the Euro-Brazilian cohort. Accordingly, Mennonites had a higher prevalence of the lactase persistence genotype PMID: 26334798
11. Populations from two medieval sites in Central Poland, Stary Brzesc Kujawski-4 (SBK-4) and Gruczno, represented high level of lactase persistence (LP) as followed by the LCT-13910*T allele's presence (0.86 and 0.82, respectively). PMID: 25853887
12. In conclusion, our data indicate that identification of a -13910C/C genotype is likely to predict the presence of lactase nonpersistence, in keeping with prior published studies. PMID: 25625576
13. Diversity of lactase persistence in African milk drinkers. PMID: 26054462
14. Evidence of selection around the LCT gene among Khoe-speaking groups, and the substantial frequency of the 14010C variant among the Nama is best explained by adaptation to digesting milk. PMID: 24704072
15. Report reliabile of the single nucleotide polymorphism of lactase persistence LPH(-13910) C/T from saliva derived DNA. PMID: 25651731
16. Genotypes of neolithic human remains indicate that natural selection models satisfy the observed increase in allele frequency in lactase persistence. PMID: 24448642
17. Evolutionary history of the European lactase persistence trait and its global cultural implications. PMID: 24465990
18. The findings of this study suggest that at least the ApaI and BsmI polymorphisms of the VDR gene and T-13910C of the LCT gene are associated with the risk of postmenopausal osteoporosis in our sample of the Belarusian women. PMID: 23985982
19. Show new sequence data from the lactase gene for two Bedouin tribal populations, the Ajman and Mutran. Study the frequency of Lactase persistence associated alleles and discuss impact of nomadic-pastoralism on the associated genetic variation. PMID: 23913618
20. The LCT enhancer sequence in a large lactose-tolerance-tested Ethiopian cohort of more than 350 individuals, was examined. PMID: 23993196
21. 580 Portuguese children (age 6-12)were genotyped for the lactase persistance-13910C>T polymorphism using TaqMan probes by real-time PCR for a link with abdominal obesity, indicating the polymorphism may predispose to abdominal obesity, not confirmed PMID: 23252911
22. The lactase persistence genotype was shown to be associated with a higher BMI. PMID: 23647908
23. Stronger signal of recent selection around the LCT gene for lactase persistence in Maasai than in Europeans. PMID: 22948027
24. An overall frequency of 0.349 for the lactase persistence (LP) - 13910*T allele was estimated in the general population, with a noticeable decrease in the South (0.269) compared with North (0.383) and Centre (0.393). Among the symptomatic group, the frequency of the - 13910*T allele (0.363) was not significantly different from the general population. PMID: 23327608
25. The two SNPs were present in a strong linkage disequilibrium. lactase persistence prevalence varied in these Indian regional groups. PMID: 23030683
26. C/T -13910 cis-acting regulatory variant located approximately 14 kb upstream of lactase gene (LCT) completely correlates with lactase phenotype in Indian children PMID: 21763294
27. The findings show that while variation in the lactase gene is associated with milk intake in men, the lactase polymorphism does not have a large effect on prostate cancer risk. PMID: 22965418
28. The study showed that though frequency of C/T-13910 and G/A-22018 lactase polymorphisms was comparable among irritable bowel syndrome (IBS) and controls; these were more common among patients with diarrhea-predominant IBS (D-IBS). PMID: 22989008
29. Lactase deficiency prevalence is high among different ethnic groups in Israel and varies between ethnicities. Both SNPs (C/T-13910 and G/A-22018) showed significant correlation in Israeli Jews of various origins. PMID: 23415628
30. Differences in the prevalence of primary lactase deficiency were not found between celiac disease patients and controls... hereditary lactase deficiency is frequent in Italian celiac disease children as in control population. PMID: 23211657
31. The researchers found evidence of the highest frequency of LCT-13915(*)G variant allele associated with lactose persistence in the southern Arabian Peninsula. PMID: 23256641
32. Single nucleotide polymorphism variants of the lactase gene in lactase persistence in the Brazilian population. PMID: 23029545
33. The T-13910 of the allele LCT-13910 C>T polymorphism is positively associated with BMI. lactase persistence increases significantly the risk to develop obesity in the studied population. PMID: 22937140
34. no significant difference in single nucleotide polymorphism between adolescent idiopathic scoliosis cases and controls PMID: 22278929
35. We confirm that the 13910 C>T mutation seen in DNA samples from across the Indian subcontinent, is identical by descent to the European allele and is associated with the same>1 Mb extended haplotype in both European and Indian populations. PMID: 21836184
36. despite not finding marked differences in dairy product consumption, this polymorphism [-13910C>T (rs4988235) upstream from the lactase (LCT) gene] was strongly associated with BMI and obesity and modulated by lactose intake... PMID: 21193851
37. The -14010*C variant associated with lactase persistence is located between an Oct-1 and HNF1alpha binding site and increases lactase promoter activity. PMID: 21327791
38. Report lactase persistence SNPs in African populations regulate promoter activity in Caco-2 cells. PMID: 21686221
39. The aim of this study was to determine the prevalence of lactase persistent and non-persistent genotypes in current Hungarian-speaking populations and in ancient Carpathian basin human bone samples. PMID: 21365615
40. The LCT-22018G>A allele is a better predictor of lactase persistence in Japanese-Brazilians than the LCT-13910C>T allele. PMID: 21340236
41. Lactase gene expression showed a regional distinction between regions 1 and 4 of the small intestine but not between regions 1 and 3. PMID: 21125297
42. the lactase gene C/T(-13910) polymorphism was associated with trabecular density at the distal radius and tibia in men. PMID: 21136048
43. the -13915*G SNP region (associated with lactase persistence) of the lactase gene interacts with the Oct-1 transcription factor in in vitro binding reactions. PMID: 20960210
44. In the Italian population the LCT-13910C>T polymorphism is not associated to the risk for colorectal cancer or polyps. PMID: 20362522
45. the -13914G > A variant is the third variant of lactase persistence and seems to correlate with lactase enzyme activity PMID: 20509822
46. Lactase persistence (LP) in 31 720 individuals from eight European population-based studies and one family study by genotyping or imputing the European LP variant, was analyzed. PMID: 20015952
47. Data show that the regulation of the trafficking kinetics and activity of domain III and entire LPH including elevation of the enzymatic activities require the correct dimerization of LPH in the ER. PMID: 19955176
48. LCT 13910 C/T polymorphism is associated with decreased serum calcium level and lower bone mineral density in postmenopausal women. PMID: 18704543
49. These data clearly demonstrate that the proregion of pro-LPH is an intramolecular chaperone that is critically essential in facilitating the folding of the intermediate form LPH beta(initial) in the context of the pro-LPH polypeptide. PMID: 11751874
50. demonstrate that GATA-5 and HNF-1alpha physically associate both in vivo and in vitro and that this interaction is necessary for cooperative activation of the lactase-phlorizin hydrolase promoter PMID: 12011060
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相關疾病：

Congenital lactase deficiency (COLACD)
亞細胞定位：

Apical cell membrane; Single-pass type I membrane protein. Note=Brush border.
蛋白家族：

Glycosyl hydrolase 1 family
組織特異性：

Intestine.
數據庫鏈接：

HGNC: 6530

OMIM: 223000

KEGG: hsa:3938

STRING: 9606.ENSP00000264162

UniGene: Hs.551506

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