KMT5A Antibody

中文名稱：

KMT5A兔多克隆抗體
貨號：

CSB-PA889082LA01HU
規格：

￥440
圖片：
Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA889082LA01HU at dilution of 1:100
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) KMT5A Polyclonal antibody
Uniprot No.：

Q9NQR1
基因名：

KMT5A
別名：

H4 K20 HMTase antibody; H4 K20 specific histone methyltransferase antibody; H4-K20-HMTase SETD8 antibody; Histone H4 K20 methyltransferase antibody; Histone lysine N methyltransferase H4 lysine 20 specific antibody; Histone-lysine N-methyltransferase SETD8 antibody; Lysine N-methyltransferase 5A antibody; N-lysine methyltransferase SETD8 antibody; Pr SET 7 antibody; Pr SET7 antibody; PR-Set7 antibody; PR/SET domain containing protein 07 antibody; PR/SET domain containing protein 8 antibody; PR/SET domain-containing protein 07 antibody; PR/SET07 antibody; PrSET7 antibody; SET 07 antibody; SET 8 antibody; SET domain containing (lysine methyltransferase) 8 antibody; SET domain containing 8 antibody; SET domain containing lysine methyltransferase 8 antibody; SET domain containing protein 8 antibody; SET domain-containing protein 8 antibody; SET07 antibody; SET8 antibody; SETD 8 antibody; setd8 antibody; SETD8_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human N-lysine methyltransferase KMT5A protein (136-258AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，KMT5A Antibody (CSB-PA889082LA01HU)，的標記方式是Non-conjugated。對于KMT5A Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA889082LB01HU	KMT5A Antibody, HRP conjugated	ELISA
FITC	CSB-PA889082LC01HU	KMT5A Antibody, FITC conjugated
Biotin	CSB-PA889082LD01HU	KMT5A Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
基因功能參考文獻：
1. Results suggest that SET domain-containing protein 8 (SET8) may be a key mediator in hyperglycemic memory. PMID: 29762637
2. that oncogenic SETD8 was regulated by miR-382 and involved glioma progression PMID: 29487005
3. Data found that KMT5A was overexpressed in papillary thyroid carcinoma (PTC) tumors. Its knockdown revealed that KMT5A participated in the proliferation, apoptosis, cell cycle, migration and invasion of PTC cells as well as attenuated fatty acid metabolism. These results provide mounting evidence of KMT5A as an oncogenic mediator in fatty acid metabolism of PTC. PMID: 29512765
4. Authors demonstrated that PR-Set7, an epigenetic regulator for H4K20me1 modification, was extensively expressed in the postnatal uteri, and its conditional deletion resulted in a complete lack of endometrial glands and infertility in mice. PMID: 28731465
5. MiR-502 medaited histone methyltransferase SET8 expression is associated with outcome of esophageal squamous cell carcinoma. PMID: 27605386
6. these date elucidated that NT21MP and miR335 mediated PR of breast cancer cells partly through regulation of Wnt/betacatenin signaling pathway. Activation of miR335 or inactivation of SETD8 could be a novel approach for the treatment of breast cancer. PMID: 28731125
7. High expression of SET8 is associated with cervical cancer. PMID: 28534991
8. This improves the binding of SAM, SAH, and sinefungin by up to 10,000-fold. A small collection of inhibitors structurally related to SAM were screened and 40 compounds were identified that only inhibit SETD8 when a nucleosome substrate is used PMID: 27369108
9. We demonstrated the expression levels of SET8 in TT genotype were higher than in CC genotypes, and high levels of SET8 were associated with poor survival in breast cancer PMID: 27144429
10. Lysine methylation represses p53 activity in teratocarcinoma cancer cells via up-regulation of SMYD2 and PR-Set7 and perpetuation of cancer cells proliferation. PMID: 27535933
11. The loss of SETD8 concurrently stimulated nucleolar function. PMID: 28249161
12. The PPARgamma-SETD8 axis constitutes an epigenetic, p53-independent checkpoint on p21-mediated cellular senescence. PMID: 28514051
13. rs16917496 polymorphism may be a risk for predisposition to prostate cancer in an Iranian population. PMID: 28578017
14. miR-502/SET8 regulatory circuit emerges as a key regulator of the pathobiology of breast cancer and a focal point for possible therapeutic intervention. PMID: 27080302
15. In non-small cell lung cancer cells, SETD8 expression suppression is involved in tumorigenesis and metastasis. PMID: 28006750
16. SET8 and ZEB1 are functionally interdependent in promoting the epithelial-mesenchymal transition and enhancing the invasive potential of prostate cancer cells in vitro. PMID: 26717907
17. SET8 expression is associated with overall survival in gastric cancer PMID: 26634528
18. analysis of a structural model for how Set8 uses multivalent interactions to bind and methylate the nucleosome based on crystallographic and solution studies of the Set8/nucleosome complex PMID: 26953260
19. This study showed SCF(beta-TRCP) earmarks Set8 for ubiquitination and degradation in a casein kinase I-dependent manner, which is activated by DNA-damaging agents. PMID: 26666832
20. Overall, the results revealed that miR-127-3p acts as a tumor suppressor and that its down-regulation in cancer may contribute to OS progression and metastasis. PMID: 26707641
21. commonality of SPS8I1-3 against SETD8, together with their distinct structures and mechanisms for SETD8 inhibition, argues for the collective application of these compounds as SETD8 inhibitors PMID: 25137013
22. SET8 is required for 53BP1 recruitment and efficient repair of DSB. PMID: 25252681
23. These data suggest that there are significant associations between the miR-502-binding site single nucleotide polymorphism in the 3'-UTR of SET8 and the TP53 codon 72 polymorphism with cervical cancer in Chinese, and there is a gene-gene interaction. PMID: 25169478
24. Our results demonstrated that SETD8 rs16917496 C/T polymorphism was associated with decreased risk of developing pediatric acute lymphoblastic leukemia PMID: 25048968
25. The SNP in the miR502 binding site of the SET8 3'-untranslated region seems to influence survival of non-Hodgkin's lymphoma. PMID: 25343552
26. significant association between the polymorphism (rs16917496) of the miR-502-binding site in the 3'-UTR of SET8 and TP53 codon 72 polymorphism and the risk of developing NSCLC. PMID: 24374662
27. Therefore, we conclude that SET8 is involved in AR-mediated transcription activation, possibly through its interaction with AR and H4K20me1 modification. PMID: 24937452
28. Genetic variation in a microRNA-502 minding site in SET8 gene confers clinical outcome of non-small cell lung cancer in a Chinese population. PMID: 24146953
29. PR-SET7 and H4K20me1 are required for establishing both the H4K16Ac and H4K20me3 marks and have a dual role in the local regulation of Pol II pausing PMID: 24459145
30. all of the H3K36-specific methyltransferases, including ASH1L, HYPB, NSD1, and NSD2 were inhibited by ubH2A, whereas the other histone methyltransferases, including PRC2, G9a, and Pr-Set7 were not affected by ubH2A. PMID: 24019522
31. Data indicate that DDX21, a nucleolar protein, was confirmed to associate with SET8. PMID: 23419719
32. a molecular mechanism for the regulation of SET8 and extend the biological function of microRNA-7 to DNA damage response PMID: 23720754
33. Data indicate the Set8-Numb-p53 signaling axis as an important regulatory pathway for apoptosis and suggests a therapeutic strategy by targeting Numb methylation. PMID: 23706821
34. miR-502-binding SNP in SET8 may modulate SET8 expression and contribute to early development of breast cancer. PMID: 23291132
35. Migration of epithelial cells is stimulated by CRL1(FBXO11)-mediated downregulation of Cdt2 and the consequent stabilization of Set8. PMID: 23478445
36. SET8 modifies hepatocellular carcinoma outcome by altering its expression, which depends, at least in part, on its binding affinity with miR-502. PMID: 22095217
37. long recognition sequence of SET8 makes it difficult to methylate a lysine in a folded region of a protein PMID: 22583696
38. PR-Set7 is an important downstream effector of CRL4(Cdt2) function during origin of DNA replication licensing, dependent on Suv4-20h1/2 activity PMID: 23152447
39. SET8 CC genotype was associated with a decreased risk of EOC in this case-control study. The analysis of genetic polymorphisms in miRNA binding sites may help identify subgroups of populations that are at high risk for EOC PMID: 22867998
40. An increase of methylated PCNA was found in cancer cells, and the expression levels of SETD8 and PCNA were correlated in cancer tissue samples PMID: 22556262
41. in breast carcinoma samples, SET8 expression is positively correlated with metastasis PMID: 21983900
42. [review] PR-Set7/Set8/KMT5a is the sole histone methyltransferase responsible for the monomethylation of histone H4 lysine 20 (H4K20me1) in higher eukaryotes. PMID: 21200139
43. SET8 is a Wnt signaling mediator and is recruited by LEF1/TCF4 to regulate the transcription of Wnt-activated genes, possibly through H4K20 monomethylation at the target gene promoters. PMID: 21282610
44. The turnover of SET8 is accelerated after ultraviolet irradiation dependent on the CRL4(CDT2) ubiquitin ligase and PCNA. PMID: 21220508
45. The results elucidate a critical role for PR-Set7 and H4K20me1 in the chromatin events that regulate replication origins. PMID: 20953199
46. PR-Set7 is transiently recruited to laser-induced DNA damage sites through its interaction with PCNA, after which 53BP1 is recruited dependent on PR-Set7 catalytic activity. PMID: 21035370
47. SET8-mediated methylation of p53 at Lys-382 promotes the interaction between L3MBTL1 and p53 in cells. PMID: 20870725
48. Results demonstrate a central role of CRL4(Cdt2)-dependent cell-cycle regulation of Set8 for the maintenance of a stable epigenetic state essential for cell viability. PMID: 20932471
49. CRL4(Cdt2)-dependent destruction of Set8 in S phase preserves genome stability by preventing aberrant chromatin compaction during DNA synthesis. PMID: 20932472
50. This study identifies CRL4-Cdt2 ubiquitin ligase to promote the ubiquitin-dependent proteolysis of the histone H4 methyltransferase Set8 during S-phase of the cell cycle and after UV-irradiation in a reaction that is dependent on PCNA. PMID: 20932471
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亞細胞定位：

Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
蛋白家族：

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, PR/SET subfamily
數據庫鏈接：

HGNC: 29489

OMIM: 607240

KEGG: hsa:387893

STRING: 9606.ENSP00000332995

UniGene: Hs.443735

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