IST1 Antibody

說(shuō)明書(shū)

中文名稱：

IST1兔多克隆抗體
貨號(hào)：

CSB-PA012171LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA012171LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA012171LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) IST1 Polyclonal antibody
Uniprot No.：

P53990
基因名：

IST1
別名：

hIST1 antibody; Increased sodium tolerance 1 antibody; Increased sodium tolerance 1 homolog antibody; IST1 homolog antibody; IST1_HUMAN antibody; KIAA0174 antibody; MAPK activating protein PM28 antibody; MGC117220 antibody; MGC128433 antibody; Putative MAPK-activating protein PM28 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human IST1 homolog protein (172-284AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，IST1 Antibody (CSB-PA012171LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于IST1 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA012171LB01HU	IST1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA012171LC01HU	IST1 Antibody, FITC conjugated
Biotin	CSB-PA012171LD01HU	IST1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.
基因功能參考文獻(xiàn)：
1. Nuclear overexpression of OLC1 was associated with clinicopathological parameters and could predict a poor overall survival in gastric adenocarcinoma. PMID: 28038462
2. The expression of OLC1 in the tumor tissues of lung adenocarcinoma is higher than that in squamous cell carcinoma. PMID: 28532543
3. Structural and biochemical studies reveal an unusually tight interaction between ULK3 and IST1, an ESCRT-III subunit required for cytokinetic abscission. PMID: 26011858
4. Overexpression of OLC1 promotes tumorigenesis of human esophageal squamous cell carcinoma. PMID: 24608342
5. Findings indicate that a high expression level of overexpressed in lung cancer 1 (OLC1) serves as a biomarker for poor prognosis for breast cancer, suggesting that OLC1 may be a potential target of antiangiogenic therapy for breast cancer. PMID: 24880589
6. Over-expression of the OCL1 is associated with colorectal cancer. PMID: 24403489
7. the binding of ALG-2 to IST1 is Ca(2+)-dependent PMID: 23649269
8. The results suggest that inclusion of IST1 into the ESCRT complex allows recruitment of spastin to promote fission of recycling tubules from the endosome. PMID: 23897888
9. OLC1 over-expression is an important factor in epithelial ovarian carcinoma prognosis and can be a potential biomarker for ovarian carcinoma. PMID: 23609236
10. The interaction between CL7MIT and CHMP1B and between CL7MIT and IST1 became stronger when IST1 or CHMP1B was additionally coexpressed, suggesting formation of ternary complex of calpain-7, IST1 and CHMP1B. PMID: 21616915
11. These results demonstrate that cigarette smoke regulates OLC1 expression in lung cancer cells by compromising its ubiquitination and subsequent degradation through the ubiquitin E3 ligase APC. PMID: 21439932
12. These data suggest that Ist1 interaction is important for spartin recruitment to the midbody and that spartin participates in cytokinesis. PMID: 20719964
13. Results demonstrate that human calpain 7 is proteolytically active, and imply that calpain 7 is activated by ESCRT-III-related protein IST1. PMID: 20849418
14. OLC1 (KIAA0174) is a candidate oncogene in lung cancer whose expression may be regulated by exposure to cigarette smoke. PMID: 19001599
15. IST1 and CHMP1 act together to recruit and modulate specific VPS4 activities required during the final stages of cell division. PMID: 19129479
16. hIST1 is essential for cytokinesis in mammalian cells. PMID: 19129480
17. Data show that the N-terminal core domains of increased sodium tolerance-1 (IST1) and charged multivesicular body protein-3 (CHMP3) form equivalent four-helix bundles, revealing that IST1 is a previously unrecognized ESCRT-III family member. PMID: 19525971
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亞細(xì)胞定位：

Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Midbody. Nucleus envelope.
蛋白家族：

IST1 family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 28977

OMIM: 616434

KEGG: hsa:9798

STRING: 9606.ENSP00000330408

UniGene: Hs.232194

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