Histone H3K4me2 Antibody

說明書

中文名稱：

Histone H3K4me2兔多克隆抗體
貨號：

CSB-PA087078
規格：

￥2630
圖片：
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K4me2 antibody.

Dot-blot analysis of all sorts of methylation peptides using H3K4me3 antibody.

Immunofluorescence analysis of 293T cell using H3K4me2antibody. Blue: DAPI for nuclear staining.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) HIST3H3 Polyclonal antibody
Uniprot No.：

Q16695
基因名：

HIST3H3
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

A synthetic methylated peptide corresponding to residues surrounding K4 of Human histone H3
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC,IF,IP,ChIP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
IP	1:50-1:200
ChIP	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin immunoprecipitation (ChIP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
基因功能參考文獻：
1. Data show that phosphatase and tensin homolog (PTEN) interacts with death domain associated protein (DAXX) and, in turn PTEN directly regulates oncogene expression by modulating DAXX-histone H3.3 (H3.3) association on the chromatin. PMID: 28497778
2. Data suggest TCF19 interacts with histone 3 lysine 4 trimethylation through its plant homeodomain finger; TCF19 expression appears to regulate gluconeogenesis in hepatocytes; TCF19 interacts with CHD4 causing NuRD complex recruitment to gene promoters of enzymes involved in gluconeogenesis. (TCF19 = transcription factor 19; CHD4 = chromodomain helicase DNA binding protein 4; NuRD = nucleosome-remodeling-deacetylase) PMID: 29042441
3. Data suggest that, during monocyte-into-macrophage differentiation, extensive trimethylation of histone H3 lysine 4 as well as histone H3 lysine 27 promotes occupancy of histone H3 at promoters of transcription factors such as HOXA (homeodomain proteins) and FOXO (forkhead transcription factors). PMID: 28304152
4. Data suggest post-translational modifications of histones, trimethylation of lysine 36 in H3 (H3K36me3) and acetylation of lysine 16 in H4 (H4K16ac), have roles in DNA damage repair; H3K36me3 stimulates H4K16ac upon DNA double-strand break; SETD2, LEDGF, and KAT5 are required for these epigenetic changes. (SETD2 = SET domain containing 2; LEDGF = lens epithelium-derived growth factor; KAT5 = lysine acetyltransferase 5) PMID: 28546430
5. Data show that tet oncogene family member 2 (TET2) cysteine-rich (CR) domain mutations disrupt the recognition of histone H3 lysine 36 (H3K36) methylation, its cellular localization, and enzyme activity. PMID: 28130413
6. Screening for H3.3 G34 mutation should therefore be recommended as a routine diagnostic marker for supratentorial central nervous system tumors PMID: 26482474
7. Data suggest chromatin exhibits histone 3 methylation markers (H3K4me3, H3K27me3) in fetal brain, heart, and liver, probably to keep progenitor cells in these organs ready for immediate differentiation. (study of 12-week aborted embryo tissues, China) PMID: 26719341
8. Regulators of the histone H3-trimethyl lysine-4 (H3K4me3) mark are significantly associated with the genetic risk architecture of common neurodevelopmental disease. PMID: 26575220
9. EP400 deposits H3.3 into chromatin alongside H2AZ and contributes to gene regulation after Pol II pre-initiation complex assembly. PMID: 26669263
10. Concurrent acetylation and methylation at H3K27 occurs in hepatocellular carcinoma cells in association with p53 abnormalities. PMID: 24614346
11. TIP60 interacted with H3K4me3 in response to TNF-alpha signaling. PMID: 25560918
12. Our data highlights the complex interplay between Nrf2 and H3S10 phosphorylation in arsenite-activated HO-1 transcription. PMID: 26291278
13. This study showed that Pediatric brainstem oligodendroglial tumors can include histone H3.3-mutated tumors and have a tendency to disseminate throughout the neuroaxis at the time of relapse. PMID: 25281433
14. Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications. PMID: 25519718
15. Histone H3.3. mutations drive pediatric glioblastoma through upregulation of MYCN. PMID: 23539269
16. our data show that histone variant H3.3 occupies distinct intranuclear chromatin domains and that these genomic loci are associated with gene expression PMID: 25482197
17. Findings suggest a role of checkpoint kinase 1 (CHK1) as an histone H3.3 serine 31 kinase. PMID: 25690891
18. histone H3 lysine 9 acetylation has a dual role in human embryonic stem cell pluripotency and neural differentiation PMID: 25519907
19. Posttranslational modifications of H3 histone (methylation) does not correlate with the sox2 expression in malignant gliomas. PMID: 25696994
20. Reptin and Pontin oligomerization and activity are modulated through histone H3 N-terminal tail interaction. PMID: 25336637
21. Deregulation of histone H3 trimethylation at lysine 27 in diffuse large B-cell lymphoma may be another high-risk phenotype PMID: 25149548
22. Data indicate that S6 kinase 2 (S6K2) can phosphorylate histone H3 at position Thr45, which may play a role during cell proliferation and/or differentiation. PMID: 23564320
23. Our data suggest that the modification and release of histones could serve markers of apoptosis in human cancer cells. PMID: 24952159
24. The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. PMID: 24324728
25. Data suggest riboflavin (RF) status regulates hepatocyte gene expression; in HepG2 cells, depletion of LSD1 (lysine (K)-specific demethylase 1A) causes aberrant gene regulation in RF-deficient cells via changes in H3 methylation at albumin promoter. PMID: 24744315
26. full-length PHF1 in HEK293 cells co-localizes with histone K27me3, but not with K36me3, and this co-localization depends on the trimethyllysine binding pocket indicating that K27me3 is an in vivo target for the PHF1 Tudor domain PMID: 23954330
27. H3K4 monomethylation establishes boundaries that restrict the recruitment of chromatin-modifying enzymes to defined regions within promoters. PMID: 24656132
28. Data indicate that MLL1 methylates Ash2L in the absence of histone H3, but only when assembled within a complex with WDR5 and RbBP5. PMID: 24235145
29. Disruption of neocortical histone H3 homeostasis by soluble Abeta implicates Alzheimer's disease. PMID: 23582659
30. Ki-67 staining is stronger than PHH3, making 'hot spots' easier to identify on ACIS. Ki-67 is more ideal than PHH3( Phosphohistone H3 ) for staining NENs, especially in tumors with borderline grades. PMID: 24021213
31. histone H3 lysine 79 dimethylation (H3K79me2) and a novel identified site, H2bK5 monomethylation (H2bK5me1), were completely absent in individuals with Neural tube defects PMID: 23376398
32. PRAME expression in leukaemic cell lines is upregulated by IFN gamma and LPS, suggesting a possible role in immune responses. Nuclear PRAME interacts with Histone H3, suggesting a role in gene regulation in the nucleus. PMID: 23460923
33. Menin-dependent transcriptional repression of histone H3 lysine 9 methylation might play an important role in preventing tumors. PMID: 23579270
34. Data using recombinant proteins in cell-free lysates suggest that interaction of survivin with phosphorylated histone 3 (H3T3-Phos; a mitotic biological marker appearing during cell division) is abolished by trimethylation at neighboring lysine. PMID: 23281010
35. SIRT2-mediated H3K18 deacetylation plays a critical role during infection, which reveals an epigenetic mechanism imposed by a pathogenic bacterium to reprogram its host. PMID: 23908241
36. The SUV39H1 chromodomain was shown to recognize histone H3K9me2/3 specifically. PMID: 23285239
37. Results suggest that histone modification in H3K27 detected using immunohistochemistry can be successfully used as an independent prognostic factor for colorectal cancer patients with metachronous liver metastasis. PMID: 23523318
38. C-kinase-activated protein phosphatase (CPI)-17 inhibitor knockdown in human pancreatic cancer cells results in dephosphorylation of histone H3. PMID: 23541585
39. Results suggest that GLP may play a significant role in the maintenance of HIV-1 latency by catalyzing dimethylation of H3K9. PMID: 23541084
40. Study shows that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex formation. PMID: 23452851
41. Histone H3 Ser-10 phosphorylation can be designated a new 'apoptotic histone code' mediated by PKCdelta. PMID: 22984491
42. Histone H3 is increased during amino acid response that is associated with active transcription. PMID: 22978410
43. The levels of H3K4me3 and H3K27me3 show dynamic changes during human oocyte maturation and preimplantation embryonic development. PMID: 22818287
44. Increased levels of decondensed chromatin in both normal progenitor cells and cancer cells are associated with global loss of H3K27me3, which is linked to MYC overexpression. PMID: 22713676
45. Elongated telomeres show increased trimethylated histone H3 Lys9 (H3K9me3)density. PMID: 22922742
46. 17beta-estradiol stimulation induces the recruitment of PAD2 to target promoters by ERalpha, whereby PAD2 then citrullinates H3R26, which leads to local chromatin decondensation and transcriptional activation. PMID: 22853951
47. Trimethylation of lysine 27 on histone H3 expression, as examined by immunohistochemistry, has the potential to be used as an immunomarker to predict nasopharyngeal carcinoma chemoradiotherapy response and patient prognosis. PMID: 21738951
48. aberration of the global H3K9me2 level is an important epigenetic event in colorectal tumorigenesis and carcinogenesis PMID: 21917293
49. our data suggest that global histone H3 and H4 modification patterns are potential markers of tumor recurrence and disease-free survival in non-small cell lung cancer PMID: 22360506
50. O-GlcNAcylation regulates mitosis-specific phosphorylations on H3, providing a mechanistic switch that orchestrates the G2-M transition of the cell cycle. PMID: 22371497
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亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

Histone H3 family
組織特異性：

Expressed in testicular cells.
數據庫鏈接：

HGNC: 4778

OMIM: 602820

KEGG: hsa:8290

STRING: 9606.ENSP00000355657

UniGene: Hs.248171

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