HS6ST1 Antibody

說(shuō)明書

中文名稱：

HS6ST1兔多克隆抗體
貨號(hào)：

CSB-PA010756LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate(30μg), SY5Y whole cell lysate(30μg), A549 whole cell lysate(30μg), Mouse skeletal muscle tissue lysate(30μg)
All lanes: HS6ST1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49,30 kDa
Observed band size: 49,30 kDa
Exposure time: 120s

Immunofluorescence staining of MCF-7 cell with CSB-PA010756LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) HS6ST1 Polyclonal antibody
Uniprot No.：

O60243
基因名：

HS6ST1
別名：

HS6ST1; HS6ST; Heparan-sulfate 6-O-sulfotransferase 1; HS6ST-1
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Heparan-sulfate 6-O-sulfotransferase 1 protein (3-133AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，HS6ST1 Antibody (CSB-PA010756LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于HS6ST1 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA010756LB01HU	HS6ST1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA010756LC01HU	HS6ST1 Antibody, FITC conjugated
Biotin	CSB-PA010756LD01HU	HS6ST1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:20-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Critical for normal neuronal development where it may play a role in neuron branching. May also play a role in limb development. May prefer iduronic acid.
基因功能參考文獻(xiàn)：
1. RT-PCR analysis showed that the overall transcriptional activity of the main Heparan Sulfate biosynthesis-involved genes (EXT1, EXT2, NDST1, NDST2, GLCE, HS2ST1, HS3ST1, HS3ST2, HS6ST1, HS6ST2, SULF1, SULF2, HPSE) was decreased by 1.5-2-fold in Grade II-III glioma. PMID: 29104277
2. HS6ST-1 and HS6ST-2 have roles in regulating the angiogenic program in ovarian cancer cells affecting HB-EGF signaling and subsequent expression of angiogenic cytokines by cancer cells PMID: 24563483
3. Hs6st1 and Hs2st generate conditions conducive to corpus callosum development. PMID: 24501377
4. idiopathic hypogonadotrophic hypogonadism--associated HS6ST1 mutations display reduced activity in vitro and in vivo, suggesting that HS6ST1 and the complex modifications of extracellular sugars are critical for normal development PMID: 21700882
5. we have characterized HS6ST-2 and HS6ST-1 human isologues, including their chromosomal localizations,HS6STs could also transfer sulphate to N -sulphoglucosamine residues located at the non-reducing terminal of HS with high affinity. PMID: 12492399
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相關(guān)疾病：

Hypogonadotropic hypogonadism 15 with or without anosmia (HH15)
亞細(xì)胞定位：

Membrane; Single-pass type II membrane protein.
蛋白家族：

Sulfotransferase 6 family
組織特異性：

Expressed in fetal brain.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 5201

OMIM: 604846

KEGG: hsa:9394

STRING: 9606.ENSP00000259241

UniGene: Hs.512841

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