HPN Antibody

中文名稱：

HPN兔多克隆抗體
貨號：

CSB-PA010704LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse liver tissue
All lanes: HPN antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa

IHC image of CSB-PA010704LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-PA010704LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品描述：

The HPN polyclonal antibody is a rabbit antibody that specifically recognizes HPN proteins from human, mouse, and rat samples. Its immunogen is the recombinant human serine protease hepsin protein (294-413aa). It has been validated for use in ELISA, WB, IHC, and IF assays. The antibody has been purified using protein G and has a purity level of over 95%.

Hepsin is a type II transmembrane serine protease that plays a key role in several physiological processes, including the regulation of blood coagulation, cell growth and differentiation, and tumor progression. It is involved in the progression of several types of cancer, including prostate, breast, and ovarian cancer.
產品名稱：

Rabbit anti-Homo sapiens (Human) HPN Polyclonal antibody
Uniprot No.：

P05981
基因名：

HPN
別名：

HPN; TMPRSS1; Serine protease hepsin; Transmembrane protease serine 1
宿主：

Rabbit
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human Serine protease hepsin protein (294-413AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，HPN Antibody (CSB-PA010704LA01HU)，的標記方式是Non-conjugated。對于HPN Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA010704LB01HU	HPN Antibody, HRP conjugated	ELISA
FITC	CSB-PA010704LC01HU	HPN Antibody, FITC conjugated
Biotin	CSB-PA010704LD01HU	HPN Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine protease that cleaves extracellular substrates, and contributes to the proteolytic processing of growth factors, such as HGF and MST1/HGFL. Plays a role in cell growth and maintenance of cell morphology. Plays a role in the proteolytic processing of ACE2. Mediates the proteolytic cleavage of urinary UMOD that is required for UMOD polymerization.
基因功能參考文獻：
1. Study showed was that Hepsin was downregulated in 78% of gastric cancer. Its high expression seems to predict poor prognosis. These results predicted that hepsin protein expression was one of the significant and independent prognostic factors for overall survival of Gastric Cancer. PMID: 27841306
2. Genetic variation in/near the gene encoding for hepsin protein may influence risk of bipolar disorder and (at least for the rs62122114-A allele) may have functional impact (i.e. differential expression) as evidenced by serum HPN protein expression. PMID: 28886448
3. significant negative association was found between hepsin expression in endometrial carcinoma cases regarding the grade and the size of tumors as well as myometrial invasion PMID: 26990747
4. These data demonstrate that the membrane-bound serine protease hepsin is the enzyme responsible for the physiological cleavage of uromodulin. PMID: 26673890
5. The Hepsin pathway acts in concert with Wnt pathway to promote prostate cancer progression. PMID: 26139199
6. Low expression levels of hepsin and TMPRSS3 are associated with poor breast cancer survival PMID: 26014348
7. Hepsin suppressed CDK11p58 internal ribosome entry site activity in prostate cancer cells by modulating UNR expression and eIF-2alpha phosphorylation. PMID: 25576733
8. The results of this study suggest that, in Korean men, some polymorphisms in the HPN gene might be associated with the risk of developing prostate cancer. PMID: 22665141
9. hepsin expression is frequently up-regulated in breast cancer tissues, which is associated with tumor growth and progression PMID: 21383634
10. These findings suggest that the MSP/RON signaling pathway may be regulated by hepsin in tissue homeostasis and in disease pathologies, such as in cancer and immune disorders. PMID: 21875933
11. Elevated levels of hepsin interfere with cell adhesion and viability in the background of prostate cancer as well as other tissue types, the details of which depend on the microenvironment provided. PMID: 21750652
12. Hepsin activity was inhibited by anthralin and increased by resveratrol. PMID: 20673210
13. Germline genetic variation of HPN does not seem to contribute to risk of prostate cancer or prognosis. PMID: 20166135
14. Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor. PMID: 19911255
15. Hepsin and maspin are inversely expressed in laser capture microdissectioned prostate cancer PMID: 12629351
16. Hepsin is functionally linked to hepatocyte growth factor/MET pathway, which may contribute to prostate cancer progression. PMID: 15792801
17. A major 11-locus haplotype is significantly associated with prostate cancer, which provides further support that HPN is a potentially important candidate gene involved in prostate cancer susceptibility. PMID: 16783571
18. the ability of hepsin to efficiently activate pro-uPA suggests that it may initiate plasmin-mediated proteolytic pathways at the tumor/stroma interface that lead to basement membrane disruption and tumor progression PMID: 16908524
19. Our findings suggest that the balance between hepsin and its inhibitor, HAI-2, may have prognostic value in RCC. PMID: 17309599
20. We analyzed all the common variations using tag SNP in the HPN and LTBP4 genes for association with IA in 390 patients and 642 controls in the Dutch population. PMID: 18487557
21. Hepsin showed potential inhibitory effects mediated by the induction of 14-3-3sigma expression which leads to both cell cycle arrest at the G2/M phase PMID: 18698500
22. Ln-332 may be one mechanism by which hepsin promotes prostate tumor progression and metastasis, possibly by up-regulating prostate cancer cell motility. PMID: 18784072
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亞細胞定位：

Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein.
蛋白家族：

Peptidase S1 family
組織特異性：

Detected in liver and kidney.
數據庫鏈接：

HGNC: 5155

OMIM: 142440

KEGG: hsa:3249

STRING: 9606.ENSP00000262626

UniGene: Hs.182385

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