HNRNPL Antibody

說明書

中文名稱：

HNRNPL兔多克隆抗體
貨號：

CSB-PA597489
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA597489(HNRNPL Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA597489(HNRNPL Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-5: MCF7 cells, 293T cells, A549 cells, Hela cells, HepG2 cells, Primary antibody: CSB-PA597489(HNRNPL Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P14866
基因名：

HNRNPL
別名：

D830027H13Rik antibody; FLJ35509 antibody; Heterogeneous nuclear ribonucleoprotein L antibody; hnRNP L antibody; hnRNP-L antibody; Hnrnpl antibody; hnRPL antibody; HNRPL_HUMAN antibody; P/OKcl.14 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthetic peptide of Human HNRNPL
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.
基因功能參考文獻：
1. High expression of HnRNP L is associated with oral squamous cell carcinoma. PMID: 27808105
2. uc.345 could upregulate the hnRNPL expression and that inhibition of (hnRNPL) dampens the tumorigenesis capability of uc.345. PMID: 27689400
3. HnRNP L is a novel regulator of HPV16 RNA processing.HnRNP L controls HPV16 RNA polyadenylation and splicing in an Akt kinase-dependent manner. PMID: 28934469
4. Translation of VEGFA mRNA in myeloid cells is dictated by a bi-directional interaction between miR-574-3p, a CA-rich microRNA, and hnRNP L. In normoxia, miR-574-3p, acting as a decoy, binds cytoplasmic hnRNP L and prevents its binding to the CARE and stimulation of VEGFA mRNA translation, simultaneously permitting miR-297-mediated translational silencing. PMID: 28520992
5. High HNRNPL expression is associated with prostate cancer. PMID: 28038443
6. hnRNP-L contributes to poor prognosis and tumor progression of BC by inhibiting the intrinsic apoptotic signaling and enhancing MAPK signaling pathways PMID: 28088793
7. hnRNP L inhibits proximal 5'SS but promotes two consecutive distal 5'SS splicing, antagonizing SRSF1 roles in KLF6 pre-mRNA splicing. PMID: 28119102
8. HNRNPL acts as the adaptor to combine the two substructures and form the intact Sam68 nuclear body through the interaction of two sets of RNA recognition motifs with the putative architectural RNA in the respective substructures. PMID: 27377249
9. hnRNP L controls inclusion of a broad spectrum of alternative cassette exons in T cells. PMID: 26437669
10. The study presents the structural characterization of the RNA recognition motif domains of hnRNP-L and demonstrate their function in repressing exon 4 of SLC2A2. PMID: 26051023
11. Data suggest that incorporation of 3(S)-methyl-beta-alanine into a short alpha-helical region of nucleic acid binding domain of hnRNP LL significantly stabilizes helix without affecting its DNA binding properties. PMID: 25982410
12. Our results reveal that hnRNP L is a new regulator for CD44 V10 exon splicing. PMID: 25623890
13. hnRNP K and hnRNP L may serve as A1CF-like cofactors in AID-mediated class switch recombination and somatic hypermutation PMID: 25902538
14. Results show that HNRNPL represses splicing when bound to intronic regions upstream of alternative exons, and in contrast, activates splicing when bound to the downstream intron. PMID: 24526010
15. hnRNP L is a potential biomarker for the diagnosis of HBV-HCC and show that hnRNP L contributes to HCC progression. PMID: 24125732
16. HnRNP L and hnRNP LL antagonistically modulate PTB-mediated splicing suppression of CHRNA1 pre-mRNA. PMID: 24121633
17. THRIL has a role in regulating TNFalpha expression through its interaction with hnRNPL PMID: 24371310
18. Data show that alternative exons with weak 5' splice site sequences specifically show a strong correlation between hnRNP L binding and hnRNP L-dependent splicing regulation. PMID: 24164894
19. functional component of hnRNP LL is consistent with the fact that the full-length hnRNP LL has a greater silencing activity than hnRNP L PMID: 23646903
20. Induction of caspase-9b expression is due to activation of hnRNP L via phosphorylation to compete/inhibit hnRNP U association with exon 3 of Casp9 mRNA. PMID: 23396972
21. hnRNP L represses CD45 exon 4 by recruiting hnRNP A1 to a sequence upstream of the 5' splice site PMID: 23394998
22. HnRNPL is a key factor involved in the spermatogenesis by functional proteomic studies of azoospermia patients with sertoli cell only syndrome. PMID: 22245417
23. Constitutive deletion of splicing factor hnRNP L impedes early embryonic development of transgenic mice. PMID: 22523384
24. NSP 5a3a's novel interaction with B23 and ribonuclear protein hnRNP-L implicates NSP 5a3a in cellular processes such as ribosome biogenesis and rRNA transcription . PMID: 20237420
25. Hypoxia induces translocation of nuclear hnRNP L to the cytoplasm, which markedly increases hnRNP L binding to VEGFA mRNA thereby inhibiting miRNA activity. PMID: 21343907
26. hnRNP L represses splicing by preventing 5' splice site recognition of the U1 snRNP. PMID: 19946215
27. The binding of hnRNP L to an exon represses strong splice sites but enhances weak splice sites. PMID: 20122404
28. identified hnRNP L as a novel Sam68-interacting protein partner. PMID: 19912651
29. AP-endonuclease 1 and hnRNP-L interact with a nCaRE-like repressor element in the AP-endonuclease 1 promoter PMID: 11809897
30. HnRNP L may be involved in the regulation of many other genes containing CA repeats or A/C-rich enhancers. PMID: 12447348
31. hnRNP L plays roles in enhancing stability, polyadenylation, and nucleocytoplasmic export; it does so by directly recruiting to intronless pre-mRNA processing enhancer (PPE) element-containing RNAs cofactors normally recruited to intron-containing RNAs. PMID: 16024770
32. 11 target genes of hnRNP L were describes. PMID: 18073345
33. hnRNP L is an essential component of CaMKIV-regulated alternative splicing through CA repeats, with its phosphorylation likely playing a critical role. PMID: 19017650
34. These results strongly demonstrate the functional requirement of cellular hnRNP L for the HCV internal ribosome entry site activity. PMID: 19061868
35. hnRNP L cross-regulates inclusion of an analogous poison exon in the hnRNP L-like pre-mRNA, which explains the reciprocal expression of the two closely related hnRNP L proteins. PMID: 19124611
36. CA repeats in the 3'UTR of bcl-2 mRNA interact with hnRNP L in vitro and in vivo; this physical association is partially involved in the decay of bcl-2 mRNA. PMID: 19298794
37. Identification of a novel, higher eukaryotic specific subunit, heterogeneous nuclear ribonucleoprotein L (HnRNP-L)required for lysine methylation in vivo. PMID: 19332550
38. confirm the interactions of eEF1A1, p54(nrb), hnRNP-L, GAPDH and ASF/SF2 with the right terminal stem-loop domain of HDV genomic RNA in vitro PMID: 19464723
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亞細胞定位：

Nucleus, nucleoplasm. Cytoplasm.
數據庫鏈接：

HGNC: 5045

OMIM: 164021

KEGG: hsa:3191

STRING: 9606.ENSP00000221419

UniGene: Hs.644906

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