HERC5 Antibody

中文名稱：

HERC5兔多克隆抗體
貨號：

CSB-PA883435LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA883435LA01HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA883435LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) HERC5 Polyclonal antibody
Uniprot No.：

Q9UII4
基因名：

HERC5
別名：

CEB1 antibody; CEBP1 antibody; Cyclin E binding protein 1 antibody; Cyclin-E-binding protein 1 antibody; E3 ISG15--protein ligase HERC5 antibody; HECT domain and RCC1 like domain containing protein 5 antibody; HECT domain and RCC1-like domain-containing protein 5 antibody; Hect domain and RLD 5 antibody; HECT E3 ubiquitin ligase antibody; HERC5 antibody; HERC5_HUMAN antibody; Herc6 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human E3 ISG15--protein ligase HERC5 protein (153-284AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，HERC5 Antibody (CSB-PA883435LA01HU)，的標記方式是Non-conjugated。對于HERC5 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA883435LB01HU	HERC5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA883435LC01HU	HERC5 Antibody, FITC conjugated
Biotin	CSB-PA883435LD01HU	HERC5 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
基因功能參考文獻：
1. Low HERC5 expression is associated with HIV infections. PMID: 29669830
2. A synonymous mutation at rs6857425 (T-C) was present in the same region among all study groups (T-C), irrespective of their HIV status. PMID: 28737979
3. Results show that HERC5 mediates covalent ISG15 conjugation to parkin in mammalian cells and that ISG15 is conjugated to the Lys349 and Lys369 residues of parkin. PMID: 27534820
4. HERC5 plays a crucial role in HCC immune evasion and has clinical relevance as a reproducible prognostic marker for risk of tumor recurrence and survival in patients. PMID: 26653219
5. The inhibitory effect of ISG15 on HCV RNA replication does not require its conjugation to substrates by HERC5. PMID: 26361997
6. vIRF1 association with HERC5 altered ISG15 modification of cellular proteins, and knockdown of ISG15 augmented reactivation of KSHV from latency. PMID: 26355087
7. The study reports on the NMR solution structure of a G-quadruplex formed by the CEB1 DNA G-rich fragment d(AGGGGGGAGGGAGGGTGG), harboring several G-tracts including one with six continuous guanines. PMID: 24742225
8. Results show that HERC5 gene may be involved in regulating the spread of non-small cell lung cancer tumors where the methylation of its promoter is correlated with number increase of disseminated tumor cells and metastases as well as survival decreased. PMID: 25353388
9. Authors identified a second distinct mechanism by which HERC5 inhibits HIV-1 replication and demonstrate that HERC5 is evolving under strong positive selection. PMID: 24693865
10. Data indicate that income was inversely related to smoking behavior, and paternally derived CEB1 mutations were dose dependently increased when the father smoked in the 6 mo before pregnancy, 0.21 vs. 0.05 in smoking and nonsmoking fathers, respectively. PMID: 23538710
11. The E3 ligase activity of HERC5 was required for blocking HIV-1 Gag particle production and correlated with the post-translational modification of Gag with ISG15. PMID: 22093708
12. This study characterizes HERC5 as a positive regulator of innate antiviral responses, and show that it sustains IRF3 activation via a novel posttranslational modification, ISG15-ylation. PMID: 20308324
13. Data show that HERC5, a functionally active HECT ubiquitin ligase, exhibits a tightly controlled cytosolic level under inflammatory conditions in endothelial cells. PMID: 15331633
14. HERC5/Ceb1 is involved in the conjugation of ISG15 to cellular proteins. PMID: 16815975
15. these results suggest that Herc5 functions as a general E3 ligase for protein ISGylation. PMID: 16884686
16. Overexpression of cyclin E is associated with neuroendocrine lung tumors PMID: 17471231
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亞細胞定位：

Cytoplasm, perinuclear region. Note=Associated with the polyribosomes, probably via the 60S subunit.
組織特異性：

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
數據庫鏈接：

HGNC: 24368

OMIM: 608242

KEGG: hsa:51191

STRING: 9606.ENSP00000264350

UniGene: Hs.26663

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