GRB2 Antibody
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中文名稱：GRB2兔多克隆抗體
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貨號：CSB-PA008951
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規格：￥1090
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圖片：
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Western Blot analysis of Hela cells using GRB2 Polyclonal Antibody
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Abundant SRC homology antibody; Adapter protein GRB2 antibody; ASH antibody; Ash protein antibody; EGFRBP GRB2 antibody; Epidermal growth factor receptor binding protein antibody; Epidermal growth factor receptor binding protein GRB2 antibody; GRB 2 antibody; GRB2 adapter protein antibody; Grb2 antibody; GRB2_HUMAN antibody; Grb3 3 antibody; Growth factor receptor bound protein 2 antibody; Growth factor receptor bound protein 3 antibody; Growth factor receptor-bound protein 2 antibody; HT027 antibody; MST084 antibody; MSTP084 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; Protein ash antibody; SEM5 antibody; SH2/SH3 adapter GRB2 antibody
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宿主：Rabbit
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反應種屬：Human,Mouse,Rat
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免疫原：Synthesized peptide derived from the Internal region of Human GRB2.
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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抗體亞型：IgG
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純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：WB, ELISA
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 ELISA 1:40000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.; Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
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基因功能參考文獻：
- that miR-1258 can suppress NSCLC progression by targeting the GRB2/Ras/Erk pathway PMID: 30069987
- The binding site of miR-433-3p was identified in the 3'UTR region of GRB2. Western blotting and FQ-PCR showed that miR-433-3p inhibited the mRNA and protein expression of GRB2. PMID: 29730656
- study unravels a unique role of Grb2 in protecting the cytoskeletal architecture in AD-like conditions and presents a potential new strategy for controlling neurodegeneration PMID: 28360125
- M. tuberculosis-initiated human mannose receptor signaling regulates macrophage recognition and vesicle trafficking by gamma Fc receptors, Grb2, and SHP-1. PMID: 28978467
- Data indicate GRB2 as a direct target of miR-329 in pancreatic cancer cells, and expression of GRB2 was inversely correlated with miR-329 expression in pancreatic cancer patients. PMID: 26885689
- EGFR colocalization with GRB2 as assessed by PLA is not correlated with EGFR expression levels or mutation status, defining a patient group that may show EGFR pathway activation, as illustrated by its prognostic value. PMID: 27449805
- Low GRB2 expression is associated with Breast Cancer. PMID: 23826330
- ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP are potent reference genes in neuroendocrine tumors of the lung. PMID: 27802291
- Following phosphorylation of the tyrosine, the proteins growth factor receptor-bound protein 2 (Grb2), Grb2-related adaptor downstream of Shc (Gads), and p85 subunit of phosphoinositide 3-kinase may bind to pYMNM (where pY is phosphotyrosine) via their Src homology 2 (SH2) domains, leading to downstream signaling to distinct immune pathways. These three adaptor proteins bind to the same site on CD28 with variable affinity PMID: 27927989
- investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation PMID: 27264952
- The study presents EPR spectroscopic analysis of the binding of the wild-type and mutant Grb2 SH2 domains to the CSpYVNVQC peptide variant. These data confirm that the binding specificity of SH2 domains, even that of Grb2, results from multiple factors and does not depend merely on the nature of one particular residue. PMID: 26645482
- TGF-beta2 induces Grb2 to recruit PI3-K to TGF-RII that activates JNK/AP-1-signaling and augments invasiveness of Theileria-transformed macrophages. PMID: 26511382
- Genetic variation near GRB2 on chromosome 17q25.1 is associated with sight-threatening diabetic retinopathy. PMID: 26188370
- Only monomeric Grb2 is capable of upregulating MAP kinase signalling. The dimeric state is inhibitory to this process. The self-association/dissociation of Grb2 represents a switch that regulates MAP kinase activity and hence controls cancer progression. PMID: 26103942
- The immunoglobulin tail tyrosine motif in the cytoplasmic segments of membrane-bound IgGs acts as the principle signal amplifier by incorporating a Grb2-Btk signaling. PMID: 25413232
- findings show that regions outside of the consensus PxxPxR sites drive the high affinity association of GRB2 with SH3 domain ligands PMID: 26079855
- down-regulated molecule growth factor receptor-bound protein 2 (GRB2) was a prominent node in fourteen cell proliferation-related sub-pathways. PMID: 25659534
- GRB2 was an independent factor in prognosis of esophageal squamous cell carcinoma patients PMID: 25031732
- In addition, GRB2 and UBC were found to be hub nodes in the differentially expressed subnetwork and may be used as potential biomarkers or targets for the diagnosis of bladder cancer. PMID: 25688510
- The N-terminal SH3 domain of Grb2 specifically interacts with UVRAG, unlike the C-terminal SH3 domain. This interaction helps to understand the role of Grb2 in the autophagic maturation of vesicles. PMID: 24882360
- Knockdown of GRB2 suppressed LMTK3-induced CDC42 activation, blocked ITGA5 and ITGB1 expression promoted by the transcription factor serum response factor (SRF), and reduced invasive activity PMID: 24939894
- these results identify Grb2 and Shc as central signaling effectors of Met-driven progression of intestinal epithelial-derived cancers. Notably, they suggest that Grb2 may represent a promising target for the design of novel colorectal cancer therapies. PMID: 24708867
- SGEF interacts with Grb2. PMID: 24399467
- Trafficking of EGFR and Grb2 in living HeLa cells stimulated with low, physiological concentrations of EGFR ligands, was analyzed.. PMID: 24259669
- In neuronal cells, amyloid-beta protein precursor intracellular domain co-localizes with GRB2 in cell compartments. PMID: 24100123
- Recruitment of the adaptor protein Grb2 to EGFR tetramers. PMID: 24697349
- suppression of Grb2 expression in HepG2 cells improved hepatic steatosis, glucose metabolism, oxidative stress, and apoptosis induced by palmitic acid incubation partly though modulating the insulin signaling pathway. PMID: 23771793
- Epigenetic repression of miR-376c accelerated EGF-dependent cell migration through its target GRB2 in HuCCT1 cells. PMID: 23922722
- a central role for adaptor proteins p66Shc and Grb2 in the regulation of ARF1 and ARF6 activation in invasive breast cancer cells. PMID: 24407288
- A deficient lipid rafts recruitment of CD3zeta/ZAP-70/Grb2, and these proteins do not merge with GM1 within the lipid rafts. PMID: 23916875
- EGFR interacts with Grb2 in both constitutive and EGF-dependent manners, whereas the HER3-Grb2 interaction requires the heteromerization of EGFR and HER3. PMID: 23700486
- VP11/12 SFK-binding motifs recruit Lck and the activated Src family kinase then leads (directly or indirectly) to phosphorylation of additional motifs involved in recruiting p85, Grb2, and Shc. PMID: 23946459
- the N-terminal SH3-SH2 region of grb2 protein was in charge of binding with phosphorylated TIGIT. PMID: 23154388
- GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. PMID: 23460737
- Grb2 is thereby recruited into the plexin-B1 receptor complex and, through its SH3 domain, interacts with p190 RhoGAP and mediates RhoA deactivation. PMID: 22505611
- Grb2 exerts constitutive control over the mutually dependent activities of FGFR2 and Shp2. PMID: 23420874
- The discovery of a negative regulatory role for Grb2 reveals that this adaptor acts as a double-edged sword in the regulation of RTK signaling. PMID: 23131845
- The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters PMID: 23223229
- The results fit with a role of Grb2 in protein aggregation, achieving specificity by multivalent interactions, despite the relatively low affinity of single SH3 interactions. PMID: 23159538
- Grb2 interacts with ADAM12 and is required for ADAM12 internalization. PMID: 22882974
- intracellular Francisella tularensis novicida triggers temporal and early activation of Ras through the SOS2/GrB2/PKCalpha/PKCbetaI quaternary complex; Ras signalling by intracellular F. tularensis is essential for intracellular proliferation in the cytosol PMID: 20618341
- the negative loop on p38 is mediated by c-ABL phosphorylation at tyrosine 105 of the adaptor protein NCK1, while the phosphorylation at tyrosine 209 of GRB2 down-modulates ERK1/2 and JNKs signaling. PMID: 22327338
- T-cell antigen receptor (TCR)-mediated Erk activation requires RasGRP1, but not Grb2/Sos. PMID: 22344067
- Modeling and simulation of aggregation of membrane protein LAT with molecular variability in the number of binding sites for cytosolic Grb2-SOS1-Grb2 PMID: 22396725
- multiple binding sites within Sos1 provide a physical route for Grb2 to hop in a flip-flop manner from one site to the next through facilitated diffusion, and such rapid exchange forms the basis of cooperativity driving bivalent binding of Grb2 to Sos1 PMID: 22360309
- Hypoxia can induce the activation of cytoplasmic FAK, Grb2 and paxillin so as to regulate the migration, survival and proliferation of pulmonary artery smooth cells. PMID: 22094095
- Our data reliably define context-specific and time-dependent networks that form around GRB2 after stimulation, and reveal core and growth factor-selective complexes comprising 90 proteins identified as interacting with GRB2 in HEK293T cells. PMID: 21706016
- ACAP4 protein cooperates with Grb2 protein to orchestrate epidermal growth factor-stimulated integrin beta1 recycling in cell migration PMID: 22027826
- High-resolution crystal structures of Grb2-SH2 domain bound to AICD peptides reveal a unique mode of binding where the peptides assume a noncanonical conformation that is unlike other structures of AICD peptides bound to protein-tyrosine-binding domains PMID: 22001015
- RhoU physically associates with activated EGFR in a GRB2-dependent manner; suppression of GRB2 by RNA interference abrogates the interaction of RhoU with activated EGFR. PMID: 21508312
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亞細胞定位：Nucleus. Cytoplasm. Endosome. Golgi apparatus.
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蛋白家族：GRB2/sem-5/DRK family
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數據庫鏈接：
