GPR34 Antibody
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中文名稱(chēng)：GPR34兔多克隆抗體
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貨號(hào)：CSB-PA891571LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: U251 whole cell lysate(30μg), U87 whole cell lysate(30μg), JK whole cell lysate(30μg), Mouse Lung tissue lysate(30μg), Mouse Stomach tissue lysate(30μg)
All lanes: GPR34 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: 120s -
IHC image of CSB-PA891571LA01HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody -
Immunofluorescence staining of U251 cell with CSB-PA891571LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of U87 cell with CSB-PA891571LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of U87 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱(chēng)：Rabbit anti-Homo sapiens (Human) GPR34 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：GPR34; Probable G-protein coupled receptor 34
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宿主：Rabbit
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反應(yīng)種屬：Human, Mouse
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免疫原：Recombinant Human Probable G-protein coupled receptor 34 protein (1-61AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁(yè)面中的產(chǎn)品，GPR34 Antibody (CSB-PA891571LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于GPR34 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：
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克隆類(lèi)型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:20-1:100 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Orphan receptor.
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基因功能參考文獻(xiàn)：
- By monitoring fused FLAG-tag and conformation-sensitive native epitope during expression of GPR34 mutants, a tri-basic motif in the first intracellular loop was identified as key topogenic signal that dictates the orientation of transmembrane domain-1. PMID: 27086875
- GPR34 knockdown impairs the proliferation and migration of HGC-27 gastric cancer cells in vitro and provides a potential implication for therapy of gastric cancer. PMID: 25673461
- up-regulation of GPR34 expression in human gastric carcinoma may play a critical role in tumor progression and in determining patient prognosis PMID: 23836308
- The present studies confirm that GPR34 is a cellular receptor for LysoPS, especially with a fatty acid at the sn-2 position. PMID: 22343749
- these results are the first to identify a role for a GPR34 in lymphoma cell growth, provide insight into GPR34-mediated signaling, identify a genetically unique subset of MZLs that express high levels of GPR34. PMID: 22966169
- data show that multiple translation initiation starts and alternative splicing contribute to the supragenomic diversification of GPR34 PMID: 16338117
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亞細(xì)胞定位：Cell membrane; Multi-pass membrane protein.
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蛋白家族：G-protein coupled receptor 1 family
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組織特異性：Broadly expressed.
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數(shù)據(jù)庫(kù)鏈接：
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