FIS1 Antibody

中文名稱：

FIS1兔多克隆抗體
貨號：

CSB-PA008684LA01HU
規(guī)格：

￥3,900
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate(20μg), Hela whole cell lysate(20μg), HepG2 whole cell lysate(20μg), JK whole cell lysate(20μg), MCF7 whole cell lysate(20μg), K562 whole cell lysate(20μg),SH-SY5Y whole cell lysate(20μg),
All lanes: FIS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size:17 kDa
Observed band size:17 kDa
Exposure time: 180s

Western Blot
Positive WB detected in: Mouse Heart tissue lysate(20μg),Mouse Brain tissue lysate(20μg)
All lanes: FIS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 60s
其他：

產(chǎn)品詳情
產(chǎn)品評價
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

The FIS1 antibody is a premium polyclonal antibody suitable for the detection of the human FIS1 protein. It is available as the unconjugated IgG isoform. This FIS1 antibody is raised in the rabbit against a partial recombinant protein corresponding to amino acids 1-122 of human FIS1. This FIS1 antibody is purified by protein G affinity chromatography with a purity of up to 95%. And it is recommended for use in ELISA, IHC, and IF applications.

Its target protein FIS1 mainly functions to regulate mitochondrial fission, morphology, and dynamics. It is involved in the recruitment of DRP1 to the mitochondrial outer membrane and plays a crucial role in mitochondrial quality control and energy metabolism. Dysregulation of FIS1 can contribute to mitochondrial dysfunction and various diseases.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) FIS1 Polyclonal antibody
Uniprot No.：

Q9Y3D6
基因名：

FIS1
別名：

2010003O14Rik antibody; CGI 135 antibody; CGI135 antibody; FIS 1 antibody; FIS1 antibody; Fis1 homolog antibody; FIS1; S. cerevisiae; homolog of antibody; FIS1_HUMAN antibody; Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody; Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody; Fission 1 (mitochondrial outer membrane) homolog antibody; Fission 1 homolog antibody; H NH0132A01.6 antibody; hFis 1 antibody; hFis1 antibody; Mitochondrial fission 1 protein antibody; mitochondrial fission molecule antibody; Tetratricopeptide repeat domain 11 antibody; Tetratricopeptide repeat protein 11 antibody; TPR repeat protein 11 antibody; TTC 11 antibody; TTC11 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Mitochondrial fission 1 protein (1-122AA)
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:3000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

Hydrogen sulfide pretreatment improves mitochondrial function in myocardial hypertrophy via a SIRT3 dependent manner Meng G.et al,Br J Pharmacol.,2017

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
基因功能參考文獻(xiàn)：
1. Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function. PMID: 27497958
2. Results show that Fis1 is significantly overexpressed in malignant oncocytomas. PMID: 25822260
3. Study revealed that a novel mitochondrial fission pathway composed of miR-483-5p and FIS1 regulates cisplatin sensitivity. PMID: 25843291
4. Overexpression of FoxM1 and Plk1 in Fis1-depleted cells restores mitotic entry. PMID: 23907611
5. Fis1 expression is significantly increased in spinal cord in a transgenic mouse model of amyotrophic lateral sclerosis. PMID: 23713734
6. Fis1 acts as a mitochondrial recruitment factor for TBC1D15 that is involved in regulation of mitochondrial morphology. PMID: 23077178
7. PEX11 proteins attract both Mff and human Fis1 (hFis1) to their site of action. PMID: 22595523
8. Fis1 and dynamin-related protein (Drp)1 levels are remarkably increased in peripheral blood lymphocytes of Alzheimer's disease patients. PMID: 22340708
9. Fis1 activity depends on its ability to interconvert between monomer and dimer species PMID: 22789569
10. The mitochondrial fission protein Fission 1 homologue (Fis1) conveys an apoptosis signal from the mitochondria to the ER by interacting with Bap31 at the ER and facilitating its cleavage into the pro-apoptotic p20Bap31. PMID: 21183955
11. we observed interactions between Drp1 and Fis1 in mitochondrial fragmentation induced by increasing mitochondrial calcium PMID: 20428767
12. hFis1 can bind to multiple amino acid sequences selectively, and the tetratricopeptide repeat constitutes the main binding region of hFis1. PMID: 19864424
13. hFis1 is part of the mammalian fission machinery and regulation of the fission processes might be involved in apoptotic mechanisms PMID: 12783892
14. regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1 PMID: 12861026
15. Cytosolic domain of fis1 adopts a tetratricopeptide repeat domain fold. PMID: 14705031
16. the level of Fis1 at the mitochondrial surface influences mitochondrial fission events and hence overall mitochondrial morphology within the cell PMID: 14996942
17. hFis1 induces mitochondrial fragmentation and perinuclear clustering in a state of calcium homeostasis PMID: 15024001
18. Fis1 together with dynamin-related protein (Drp1) antogonizes Bcl-2. PMID: 16010987
19. These findings provide the first evidence for a role of Fis1 in peroxisomal fission and suggest that the fission machinery of mitochondria and peroxisomes shares common components. PMID: 16107562
20. TPR region of hFis1 participates in the interaction with DLP1 or DLP1-containing complex and that the first helix (alpha1) of hFis1 is required for mitochondrial fission presumably by regulating DLP1-hFis1 interaction. PMID: 16118244
21. Direct induction of mitochondrial elongation by blocking mitochondrial fission process with Fis1-DeltaTM or Drp1-K38A was sufficient to develop senescent phenotypes with increased Reactive oxygen species production. PMID: 16883569
22. hFis1 is a bifunctional protein that independently regulates mitochondrial fragmentation and endoplasmic reticulum-mediated apoptosis. PMID: 16914522
23. Fis1 plays important roles in peroxisome division and maintenance of peroxisome morphology in mammalian cells, possibly in a concerted manner with Pex11pbeta and DLP1. PMID: 17408615
24. hFis1 and OPA1 modulate cellular senescence PMID: 17545159
25. the concave surface of the Fis1 tetratricopeptide repeat-like domain is evolutionarily conserved to bind the dynamin-like GTPase Dnm1 and not Mdv1 as previously predicted PMID: 17884824
26. Changes correlate with mitochondrial dysfunction rather than with fragmentation, as substantiated by Fis1 mutants with different effects on organelle shape and function PMID: 18515060
27. targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins. PMID: 18782765
28. Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID: 18832378
29. oligomerization of hFis1 in the mitochondrial outer membrane plays a role in mitochondrial fission, potentially through participating in fission factor recruitment. PMID: 18845145
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亞細(xì)胞定位：

Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
蛋白家族：

FIS1 family
數(shù)據(jù)庫鏈接：

HGNC: 21689

OMIM: 609003

KEGG: hsa:51024

STRING: 9606.ENSP00000223136

UniGene: Hs.423968

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