FAN1 Antibody

中文名稱：

FAN1兔多克隆抗體
貨號：

CSB-PA22859A0Rb
規格：

￥440
圖片：
Western Blot
Positive WB detected in: PC-3 whole cell lysate
All lanes: FAN1 antibody at 5.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 115, 60 kDa
Observed band size: 115 kDa

IHC image of CSB-PA22859A0Rb diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品描述：

The product CSB-PA22859A0Rb is a rabbit polyclonal antibody raised against recombinant human FAN1 (167-373aa). This FAN1 antibody is purified by protein G and gets a purity of up to 95%. It can recognize and detect human FAN1 protein in ELISA, WB, and IHC applications.

The FAN1 protein acts as a nuclease enzyme that plays a critical role in the repair of DNA damage, especially the interstrand crosslinks (ICLs). It has also been implicated in other cellular processes, including telomere maintenance and genome stability. Dysregulation of FAN1 expression or function has been associated with various pathological conditions, including cancer, neurodegenerative diseases, and autoimmune disorders.
產品名稱：

Rabbit anti-Homo sapiens (Human) FAN1 Polyclonal antibody
Uniprot No.：

Q9Y2M0
基因名：

FAN1
別名：

6030441H18Rik antibody; Coiled coil domain containing protein MTMR15 antibody; DKFZp451H236 antibody; DKFZp686K16147 antibody; FAN1 antibody; FAN1_HUMAN antibody; FANCD2 and FANCI associated nuclease 1 antibody; FANCD2/FANCI associated nuclease 1 antibody; FANCD2/FANCI-associated nuclease 1 antibody; Fanconi associated nuclease 1 antibody; Fanconi-associated nuclease 1 antibody; hFAN1 antibody; KIAA1018 antibody; KMIN antibody; MTMR 15 antibody; MTMR15 antibody; Myotubularin related protein 15 antibody; Myotubularin-related protein 15 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Fanconi-associated nuclease 1 protein (167-373AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，FAN1 Antibody (CSB-PA22859A0Rb)，的標記方式是Non-conjugated。對于FAN1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA22859B0Rb	FAN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA22859C0Rb	FAN1 Antibody, FITC conjugated
Biotin	CSB-PA22859D0Rb	FAN1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps.
基因功能參考文獻：
1. hFAN1 homodimerization plays a role in biological processes that involve 5' DNA Flap cleavage. PMID: 29518739
2. FAN1 interaction with ubiquitylated PCNA alleviates replication stress and preserves genomic integrity independently of BRCA2 PMID: 29051491
3. The structures, function, and proposed mechanisms of FAN1 nuclease are discussed, and the insights into its role in interstrand cross-links repair and in processing of stalled replication forks are provided. [REVIEW] PMID: 28623094
4. show that DNA repair genes (fan1 and pms2) significantly modify age at onset in Huntington's Disease and Spinocerebellar Ataxias, suggesting a common pathogenic mechanism, which could operate through the observed somatic expansion of repeats PMID: 27044000
5. FAN1 efficiently promoted DNA incision at the proper site of RPA-coated 5'-flapped DNA. Therefore, FAN1 possesses the ability to promote the ICL repair of 5'-flapped DNA covered by RPA. PMID: 25922199
6. EXO1 and FEN1 cleaved the substrate at the boundary between the single-stranded 5' flap and the duplex, whereas FAN1 incised it three to four nucleotides in the double-stranded region. PMID: 26221031
7. Detected FAN1 mutations in approximately 3% of families who met the Amsterdam criteria for hereditary colorectal cancer and had mismatch repair-proficient cancers with no previously associated mutations. PMID: 26052075
8. The crystal structures of human FAN1 in complex with a 5' flap DNA substrate show that two FAN1 molecules form a head-to-tail dimer to locate the lesion, orient the DNA, and unwind a 5' flap for subsequent incision. PMID: 25500724
9. In this work, FAN1-DNA crystal structures and biochemical data reveal that human FAN1 cleaves DNA successively at every third nucleotide PMID: 25430771
10. Results show that FAN1 utilizes its nuclease activity-in cooperation with the BLM-FANCD2 complex-to promote replication fork restart and simultaneous suppression of new origin firing. PMID: 25135477
11. FAN1 encodes a DNA repair enzyme, thus implicating abnormalities in DNA repair in the susceptibility to schizophrenia or autism PMID: 24344280
12. FAN1 might be a new mitotic substrate of APC/CCdh1 that plays a key role during mitotic exit. PMID: 22854063
13. By exome sequencing, we identified mutations in FAN1 as a cause of karyomegalic interstitial nephritis, a disorder that serves as a model for renal fibrosis. PMID: 22772369
14. Our results suggest that FAN1 has a minor role in interstrand crosslink repair compared with true Fanconi anemia genes and exclude FAN1 as a novel FA gene. PMID: 22611161
15. Two FAN1 variants do not appear to be causal for breast cancer. PMID: 21858661
16. Data show that FAN1 in DT40 cells participates in the processing of damage induced by interstrand cross-linking-generating agents also independently of the classical FA pathway. PMID: 21115814
17. Data show that FAN1 is a nuclear protein and forms DNA-damage-induced foci, which appear to be at stalled replication forks as denoted by RPA colocalization. PMID: 20935496
18. study characterizes FANI which promotes DNA interstrand cross-linking repair in a manner strictly dependent on its ability to accumulate at or near sites of DNA damage and that relies on mono-ubiquitylation of the FANCI-FANCD2 complex PMID: 20671156
19. Study describes how a highly conserved protein, KIAA1018/MTMR15/FAN1, that interacts with, and is recruited to sites of DNA damage by, the monoubiquitinated form of FANCD2. PMID: 20603015
20. KIAA1018 is a 5'-->3' exonuclease and a structure-specific endonuclease that preferentially incises 5' flaps; like cells from FA patients, human cells depleted of KIAA1018 are sensitized to ICL-inducing agents and display chromosomal instability. PMID: 20603016
21. FAN1 colocalizes at sites of DNA damage with the ID complex in a manner dependent on FAN1's ubiquitin-binding domain (UBZ), the FANCI-FAND2 (ID) complex, and monoubiquitination of FANCD2. PMID: 20603073
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相關疾病：

Interstitial nephritis, karyomegalic (KMIN)
亞細胞定位：

Nucleus.
蛋白家族：

FAN1 family
數據庫鏈接：

HGNC: 29170

OMIM: 613534

KEGG: hsa:22909

STRING: 9606.ENSP00000354497

UniGene: Hs.584863

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