ERI1 Antibody
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中文名稱：ERI1兔多克隆抗體
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貨號(hào)：CSB-PA22449A0Rb
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: ERI1 antibody at 7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 46 kDa -
IHC image of CSB-PA22449A0Rb diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA22449A0Rb diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA22449A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) ERI1 Polyclonal antibody
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Uniprot No.：
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基因名：ERI1
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別名：3' 5' exonuclease ERI 1 antibody; 3' 5' exonuclease ERI1 antibody; 3' EXO antibody; 3' exoribonuclease antibody; 3' HEXO antibody; 3' histone mRNA exonuclease 1 antibody; 3''-5'' exonuclease eri1 antibody; 3''-5'' exoribonuclease 1 antibody; 3'EXO antibody; 3'HEXO antibody; ERI 1 antibody; Eri 1 homolog antibody; Eri-1 homolog antibody; eri1 antibody; Eri1 homolog antibody; ERI1_HUMAN antibody; HEXO antibody; Histone mRNA 3' end specific exonuclease antibody; Histone mRNA 3' end specific exoribonuclease antibody; Histone mRNA 3''-end-specific exoribonuclease antibody; Histone mRNA 3''-exonuclease 1 antibody; MGC35395 antibody; Protein 3''hExo antibody; THEX 1 antibody; THEX1 antibody; Three prime histone mRNA exonuclease 1 antibody
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宿主：Rabbit
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反應(yīng)種屬：Human, Rat
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免疫原：Recombinant Human 3\'-5\' exoribonuclease 1 protein (2-66AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁(yè)面中的產(chǎn)品，ERI1 Antibody (CSB-PA22449A0Rb)，的標(biāo)記方式是Non-conjugated。對(duì)于ERI1 Antibody，我們還提供其他標(biāo)記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
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基因功能參考文獻(xiàn)：
- the crystal structure of a ternary complex of human SLBP RNA binding domain, human 3'hExo, and a 26-nucleotide stem-loop RNA is reported. PMID: 23329046
- 3'hExo is a primary candidate for the exonuclease that initiates rapid decay of histone mRNA upon completion and/or inhibition of DNA replication PMID: 14536070
- molecular cloning; degrades siRNAs in vitro[ERI-1] PMID: 14961122
- Data describe the structure of the nuclease domain of 3'-5'-exoribonuclease (3'hExo) complexed with rAMP in the presence of magnesium at 1.6 A resolution. PMID: 15451662
- 3'hExo is a 3' exonuclease specifically interacting with the 3' end of histone mRNA PMID: 16912046
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亞細(xì)胞定位：Cytoplasm. Nucleus. Nucleus, nucleolus.
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數(shù)據(jù)庫(kù)鏈接：
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