E2F4 Antibody

說明書

中文名稱：

E2F4兔多克隆抗體
貨號：

CSB-PA290646
規格：

￥2024
圖片：
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using E2F4 antibody.

Western blot analysis of extracts from COS7 cells, using E2F4 antibody.

Western blot analysis of extracts from JK cells (Lane 2), using E2F4 antiobdy. The lane on the left is treated with synthesized peptide.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) E2F4 Polyclonal antibody
Uniprot No.：

Q16254
基因名：

E2F4
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human E2F4.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:3000
IHC	1:50-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
基因功能參考文獻：
1. E2f4 forms apical cytoplasmic organizing centres for assembly and nucleation of deuterosomes. Using genetically altered mice and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these organizing centres and that, without cytoplasmic E2f4, deuterosomes are not assembled, halting multiciliogenesis PMID: 28675157
2. Studied expression of E2F4 in breast cancer patients undergoing neoadjuvant chemotherapy; found target gene-based signature of E2F4 can be used to predict neoadjuvant response. PMID: 28464832
3. The authors found that phosphorylation of residues S650 and S975 in p107 weakens the E2F4 transactivation domain binding. PMID: 27567532
4. E2F4 gene expression in glioblastoma. PMID: 27983535
5. This study found evidence that the number of triplet AGC repeats in the E2F4 gene may play a role in the susceptibility to early-onset colorectal cancer. PMID: 26343152
6. PHF8 reduces the H3K9me2 level at the E2F4 transcriptional start site, demonstrating a direct function of PHF8 in endothelial E2F4 gene regulation PMID: 26751588
7. Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 proteins. PMID: 25650659
8. E2F4 promoter occupancy is globally associated with p53-repression targets, but not with p53 activation targets. PMID: 24096481
9. cancer-associated E2F4 mutations enhance the capacity of colorectal cancer cells to grow without anchorage, thereby contributing to tumor progression. PMID: 24100580
10. Short alleles (<13 repeats) of (AGC)n in E2F4 were less frequent in women with breast cancer than in the control sample. PMID: 23015403
11. the loss of CDH1/E2F4 may be associated with worse clinical and pathological findings in mammary ductal carcinoma. PMID: 23007606
12. In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component. PMID: 23093672
13. Silica could induce the high expression of cyclin D1 and CDK4 and the low expression of E2F-4, resulting in the cell cycle changes by AP-1/cyclin D1 pathway in human embryonic lung fibroblasts. PMID: 22357515
14. data demonstrate that enforced E2F4 expression in Burkitt lymphoma (BL) cells not only diminishes E2F1 levels, but also reduces selectively the tumorigenic properties and proliferation of BL cells PMID: 22475873
15. Our data indicate that E2F4 is required for cardiomyocyte proliferation and suggest a function for E2F4 in mitosis PMID: 19955219
16. E2F4, PHACTR3, PRAME family member and CDH12 most probably play important role in non-small-cell lung cancer geneses PMID: 19473719
17. regulation of expression of p130, p107 and E2F-4 in human cells PMID: 12006580
18. data point to Tat as an adaptor protein that recruits cellular factors such as E2F-4 to exert its multiple biological activities PMID: 12055184
19. Mutations in E2F-4 gene is associated with hereditary non-polyposis colorectal cancer PMID: 12148576
20. EBV LMP1 blocks p16INK4 pathway by promoting nuclear export of E2F-4. PMID: 12860972
21. E2F-a and E2F-c binding sites are involved in the TCR-induced down-regulation of ICBP90 gene transcription PMID: 15964557
22. E2F4 is a transcriptional regulator of the cell cycle genes. PMID: 16135806
23. Hypoxia induces substantial p130 dephosphorylation and nuclear accumulation, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
24. in response to radiation, E2F4 becomes active in the nucleus, enforces a stable G(2) arrest by target gene repression, and thus provides increased cell survival ability by minimizing propagation of cells that have irreparable DNA damage PMID: 17043659
25. Evolutionarily conserved multisubunit protein complex that contains p130 and E2F4 mediates the repression of cell cycle-dependent genes in quiescence. PMID: 17531812
26. deregulated nuclear E2F4 expression induces apoptosis via multiple pathways in normal intestinal epithelial cells but not in colon cancer cells. PMID: 17656449
27. E2F4, binding sites are located within 2 kb of a transcription start site, in both normal and tumor cells PMID: 17908821
28. Cell cycle genes are the evolutionarily conserved targets of the E2F4 transcription factor PMID: 17957245
29. FBI-1 is the first transcriptional repressor shown to act as a dual regulator in adipogenesis exerting repressor activities on target genes by both, direct and indirect mechanisms. PMID: 18368381
30. E2F4 may be determinant in the promotion of proliferation of human intestinal epithelial crypt cells and colorectal cancer cells. PMID: 19562678
31. By interacting with p130, E2F4 plays a key role in the maintenance of a stable G2 arrest. Increased E2F4 levels and its translocation to the nucleus following genotoxic stress result in downregulation of mitotic genes. PMID: 17507799
32. Increased E2F4/p130 complex formation seen after irradiation depended on increased nuclear E2F4, dissociation of p130 from Cdk2, and p130 dephosphorylation. E2F4 siRNA prevented p130/E2F4 formation and sensitized cells to radiation-induced apoptosis. PMID: 15231644
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亞細胞定位：

Nucleus.
蛋白家族：

E2F/DP family
組織特異性：

Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
數據庫鏈接：

HGNC: 3118

OMIM: 600659

KEGG: hsa:1874

STRING: 9606.ENSP00000368686

UniGene: Hs.108371

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