DNPEP Antibody

中文名稱：

DNPEP兔多克隆抗體
貨號：

CSB-PA007087LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA007087LA01HU diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-PA007087LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DNPEP Polyclonal antibody
Uniprot No.：

Q9ULA0
基因名：

DNPEP
別名：

Aspartyl aminopeptidase antibody; ASPEP antibody; DAP antibody; DNPEP antibody; DNPEP_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Aspartyl aminopeptidase protein (279-386AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，DNPEP Antibody (CSB-PA007087LA01HU)，的標記方式是Non-conjugated。對于DNPEP Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA007087LB01HU	DNPEP Antibody, HRP conjugated	ELISA
FITC	CSB-PA007087LC01HU	DNPEP Antibody, FITC conjugated
Biotin	CSB-PA007087LD01HU	DNPEP Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism.
基因功能參考文獻：
1. aspartyl aminopeptidase was upregulated in colorectal cancer tissues, and greater activity correlated significantly with the absence of lymph node metastases and with better overall survival PMID: 23948443
2. The crystal structure of human DNPEP complexed with zinc and a substrate analogue aspartate-beta-hydroxamate reveals a dodecameric machinery built by domain-swapped dimers. PMID: 22720794
3. Identification of histidine residues important in the catalysis and structure of aspartyl aminopeptidase PMID: 12413488
亞細胞定位：

Cytoplasm.
蛋白家族：

Peptidase M18 family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 2981

OMIM: 611367

KEGG: hsa:23549

STRING: 9606.ENSP00000273075

UniGene: Hs.258551

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