DMGDH Antibody

中文名稱：

DMGDH兔多克隆抗體
貨號：

CSB-PA883415LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Mouse liver tissue, Mouse kidney tissue
All lanes: DMGDH antibody at 6.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 97, 46 kDa
Observed band size: 97 kDa

Immunofluorescence staining of HepG2 cells with CSB-PA883415LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA883415LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) DMGDH Polyclonal antibody
Uniprot No.：

Q9UI17
基因名：

DMGDH
別名：

Dimethylglycine dehydrogenase antibody; Dimethylglycine dehydrogenase; mitochondrial antibody; Dmgdh antibody; M2GD_HUMAN antibody; ME2GLYDH antibody; mitochondrial antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Dimethylglycine dehydrogenase, mitochondrial protein (429-524AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，DMGDH Antibody (CSB-PA883415LA01HU)，的標(biāo)記方式是Non-conjugated。對于DMGDH Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA883415LB01HU	DMGDH Antibody, HRP conjugated	ELISA
FITC	CSB-PA883415LC01HU	DMGDH Antibody, FITC conjugated
Biotin	CSB-PA883415LD01HU	DMGDH Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.
基因功能參考文獻(xiàn)：
1. In nonalcoholic fatty liver disease patient, the missense variant p.Ser646Pro (rs1805074) in DMGDH gene was significantly associated with disease severity and circulating levels of dimethylglycine. PMID: 27614103
2. The structure-based analysis provided new insights into the kinetic properties of dimethylglycine dehydrogenase in particular with respect to oxygen reactivity. PMID: 27486859
3. In agreement with previous studies, we show that the genetic variant rs921943 in DMGDH is significantly associated with selenium status in United Kingdom pregnant women. PMID: 26675765
4. Genetic variation of DMGDH was associated with higher plasma insulin, increased insulin resistance and increased risk of incident diabetes. PMID: 25795213
5. analysis of dimethylglycine dehydrogenase deficiency associated with pathogenic variant H109R PMID: 18937046
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相關(guān)疾病：

DMGDH deficiency (DMGDHD)
亞細(xì)胞定位：

Mitochondrion.
蛋白家族：

GcvT family
數(shù)據(jù)庫鏈接：

HGNC: 24475

OMIM: 605849

KEGG: hsa:29958

STRING: 9606.ENSP00000255189

UniGene: Hs.655653

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