Cleaved-NOTCH4 (V1432) Antibody

說明書

中文名稱：

Cleaved-NOTCH4 (V1432)兔多克隆抗體
貨號：

CSB-PA020146
規格：

￥1090
圖片：
Western Blot analysis of A549 NIH-3T3 SH-SY5Y K562 HELA cells using Cleaved-Notch 4 (V1432) Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q99466
基因名：

NOTCH4
別名：

FLJ16302 antibody; hNotch 4 antibody; hNotch4 antibody; INT3 antibody; Neurogenic locus notch homolog protein 4 antibody; NOTC4_HUMAN antibody; Notch 4 antibody; Notch 4 intracellular domain antibody; Notch homolog 4 antibody; Notch4 antibody; NOTCH4 protein antibody
宿主：

Rabbit
反應種屬：

Human,Monkey
免疫原：

Synthesized peptide derived from the C-terminal region of Human Notch 4.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:5000

Protocols：

Western Blotting (WB) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system.
基因功能參考文獻：
1. Results provide evidence that Notch4 plays an important role in vasculogenic mimicry formation and tumor invasion in hepatocellular carcinoma. PMID: 29058285
2. The results of the present study show that RNA interference targeting against Notch-4 expression suppresses prostate cancer progression. PMID: 28374086
3. Very rare missense variant in the regulatory NODP domain of NOTCH4 located at the 6p21 locus was identified in a single family.This family suggests a novel mechanism of systemic sclerosis pathogenesis in which a rare and penetrant coding variation can substantially elevate disease risk in contrast to the more modest non-coding variation typically found at this locus. PMID: 27829420
4. High NOTCH4 expression is associated with preeclampsia. PMID: 27069008
5. Data suggest that the vascular DLL4-Notch4 signaling and VEGF signaling complementing each other plays an important role in the progression of tumor angiogenesis in primary glioblastoma. PMID: 26472724
6. findings indicate that Notch4+ melanoma cancer stem-like cells trigger epithelial-mesenchymal transition and promote the metastasis of melanoma cells PMID: 27234159
7. the aberrant activation of Notch4 promotes oral squamous cell carcinoma metastasis. PMID: 27197026
8. data are consistent with those obtained in previous studies that suggested that rs204993 is associated with schizophrenia and that the AA genotype of rs204993 demonstrates a higher risk PMID: 26605328
9. High Notch4 expression is associated with Prostatic Intraepithelial Neoplasia. PMID: 26341090
10. Anti-estrogen resistance in human breast tumors is driven by JAG1-NOTCH4-dependent cancer stem cell activity. PMID: 26387946
11. NOTCH4 polymorphism can influence clinical symptoms in Slovenian patients with schizophrenia. PMID: 25529856
12. Our findings provide a novel mechanism for Notch4-ICD regulation, suggesting a negative regulatory role for the PI3K-AKT pathway in Notch4 nuclear signaling. PMID: 25740432
13. This study failed to replicate the significant association between the NOTCH4 gene rs3131296 polymorphism and the risk for schizophrenia. PMID: 25142293
14. BITC-mediated inhibition of bCSC was augmented by knockdown of Notch4 and Nicastrin, but not by RNA interference of Notch1 or Notch2. PMID: 25663545
15. Study shows that Notch4 activation can promote gastric cancer growth by inducing Wnt1/b-catenin signaling. PMID: 25511451
16. A genome-wide significant association with cryoglobulin-related vasculitis was identified with SNPs near NOTCH4. PMID: 25030430
17. This paper demonstrated that Nicastrin and Notch4 are key molecules involved in resistance to endocrine therapy. The data suggest that targeting Notch4 and Nicastrin is a potential approach to reverse endocrine resistance in breast cancer patients. PMID: 24919951
18. The associations of rs2616984 in CSMD1 gene, putative associations of rs3131296 in NOTCH4 gene, and associations of rs2229741 of NRIP1 gene with Alzheimer's disease have been found in a Russian population. PMID: 25845235
19. The expression of NOTCH4 in the dorsolateral prefrontal cortex and amygdala is decreased in suicide victims compared to control brain. PMID: 24151014
20. High Notch4 expression is associated with glioblastoma multiforme. PMID: 24305720
21. This NOTCH4 missense mutation decreased the risk for developing multiple sclerosis in Japanese, but did not affect clinical features of those who had already developed the disease PMID: 23549433
22. A possible contribution of Notch4 signalling to the development of brain arteriovenous malformations in human. PMID: 24373503
23. NOTCH4 overexpression is associated with triple-negative breast cancer. PMID: 24403446
24. RNAi directed against Notch-4 markedly decreased Notch-4 gene expression. PMID: 23450325
25. The results of this study provided stringent support for previous genetic findings reporting the association of an intronic SNP (rs3131296, chr6: 32 172 993 bp on hg19) in NOTCH4 in a European cohort. PMID: 22641179
26. The length of (CA)n in EGFR and (CTG)n in NOTCH4 was not associated with breast cancer (OR=0.99; 95% CI 0.59-1.37; p=0.619 and OR=1.08; 95% CI 0.71-1.65; p=0.725, respectively). PMID: 23015403
27. Our study shows that genetic variations within the NOTCH4 gene significantly modify the clinical characteristics of migraine and may have a role in disease pathogenesis. PMID: 23566281
28. We provide the first genetic evidence suggesting the involvement of NOTCH4 polymorphisms in rheumatoid inflammatory process. PMID: 23318300
29. Notch4-dependent antagonism of canonical TGF-beta1 signaling defines unique temporal fluctuations of SMAD3 activity in proximal tubular epithelial cells exposed to shear stress. PMID: 23576639
30. These data suggest that N4IC promoted proliferation through modulating the ERK pathway and the cell cycle during the early stage of 3T3-L1 adipogenesis and facilitated differentiation through up-regulating adipogenic genes such as C/EBPalpha, PPARgamma, aP2, LPL and HSL during the middle and late stages of 3T3-L1 adipogenesis. PMID: 23237809
31. strong association between expression and bipolar disorder PMID: 23212060
32. Genome-wide association study of age-related macular degeneration identifies TNXB, FKBPL and NOTCH4 as candidate susceptibility genes. PMID: 22694956
33. Functional interaction of Notch4(ICD) and Elongin C provides novel insight into regulation of Notch signaling in epithelial cell biology and disease. PMID: 22001063
34. the NOTCH4 polymorphisms, the minor allele of rs422951 and the 12-repeat variation for rs72555375, can be considered to be markers for sporadic inclusion body myositis susceptibility PMID: 22732452
35. The NOTCH4 polymorphisms most strongly associated with schizophrenia exert their effects on susceptibility by altering the efficiency and/or alternative splicing of Notch4 transcripts. PMID: 22488909
36. arachidonic acid significantly decreased methylation of the promoters of both KDR and Notch4 genes in Human umbilical vein endothelial cells. PMID: 21864059
37. Only rs17604492 single nucleotide polymorphism of NOTCH4 gene was found to be weakly associated with susceptibility to Kawasaki Disease (KD) in a Korean population. Results indicate NOTCH4 doesn't contribute to susceptibility to KD. PMID: 21203870
38. This research examined the methylation status of a region surrounding the NOTCH4 -25 C/T site in leukocyte genomic DNA and human brain regions. PMID: 21085055
39. Studies indicate that different receptors and ligands expression in breast cancer and various ways in which the pathway can be inhibited. PMID: 21045140
40. Notch-4 may play an important role in salivary adenoid cystic carcinoma. PMID: 20596622
41. Notch 4 receptors and their ligands play differential roles in the cytodifferentiation of squamous odontogenic tumors of the mandible. PMID: 20554499
42. Notch4 signaling activity was 8-fold higher in breast cancer stem cell-enriched cell populations compared with differentiated cells, whereas Notch1 signaling activity was 4-fold lower in the stem cell-enriched cell populations. PMID: 20068161
43. The Notch gene family regulates embryonic cell migration, vascular morphogenesis and remodeling. The Notch 4 locus has been implicated in schizophrenia, but this study rules out a significant role for the Notch4 gene in schizophrenia in the Han Chinese. PMID: 11803454
44. Genetic analysis of (CTG)n polymorphism in bipolar pedigrees PMID: 11901359
45. Activated Notch4 inhibits angiogenesis: role of beta 1-integrin activation. PMID: 11909975
46. significant association of alopecia areata in the overall data set with the Notch4(T+1297C) polymorphism (P<0.001) but not with Notch4(A+3063G) PMID: 12589427
47. The investigated NOTCH4 variants do not have a major influence on susceptibility to schizophrenia or related neurobiological traits. PMID: 12605097
48. Findings may reflect the interaction of NOTCH4 with the underlying genetic and phenotypic complexity that characterizes both schizophrenia and normal cognition and brain development. PMID: 12627456
49. NOTCH4 T-25C polymorphism has an important effect on the age of onset in schizophrenia. PMID: 12782960
50. Notch4 signaling in vascular endothelial cells. PMID: 12814948
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.; [Notch 4 intracellular domain]: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
蛋白家族：

NOTCH family
組織特異性：

Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukoc
數據庫鏈接：

HGNC: 7884

OMIM: 164951

KEGG: hsa:4855

STRING: 9606.ENSP00000364163

UniGene: Hs.436100

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