COX5B Antibody

說(shuō)明書(shū)

中文名稱(chēng)：

COX5B兔多克隆抗體
貨號(hào)：

CSB-PA00404A0Rb
規(guī)格：

￥440
圖片：
Western blot
All lanes: COX5B antibody at 2µg/ml + EC109 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 14 kDa
Observed band size: 14, 65 kDa

Immunofluorescent analysis of MCF-7 cells using CSB-PA00404A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱(chēng)：

Rabbit anti-Homo sapiens (Human) COX5B Polyclonal antibody
Uniprot No.：

P10606
基因名：

COX5B
別名：

COX 5B antibody; COX5B antibody; COX5B_HUMAN antibody; COXVB antibody; Cytochrome c oxidase polypeptide Vb antibody; Cytochrome c oxidase polypeptide VB mitochondrial antibody; Cytochrome c oxidase subunit 5B antibody; Cytochrome c oxidase subunit 5B mitochondrial antibody; Cytochrome c oxidase subunit Vb antibody; mitochondrial antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Cytochrome c oxidase subunit 5B, mitochondrial protein (32-129AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，COX5B Antibody (CSB-PA00404A0Rb)，的標(biāo)記方式是Non-conjugated。對(duì)于COX5B Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱(chēng)	應(yīng)用
HRP	CSB-PA00404B0Rb	COX5B Antibody, HRP conjugated	ELISA
FITC	CSB-PA00404C0Rb	COX5B Antibody, FITC conjugated
Biotin	CSB-PA00404D0Rb	COX5B Antibody, Biotin conjugated	ELISA

克隆類(lèi)型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
基因功能參考文獻(xiàn)：
1. Our results suggest that COX5B protein level might be associated with tumor size. COX5B overexpression indicated a worse DFS (p < 0.05) in breast cancer. PMID: 28592145
2. These experiments suggest that the FAD group of cytochrome b5 reductase increase its solvent exposition upon complex formation promoting an efficient electron transfer between the proteins. PMID: 29111436
3. silence of COX5B leads to metabolic disorders, such as increased glucose uptake and decreased lactate secretion PMID: 26506233
4. Transfected the A549 cells with COX Vb small interfering or shRNA and observed a significant reduction of their COX activity, oxygen consumption, ATP and ability to grow in soft agar and as poorly differentiated tumors in athymic mice. PMID: 22917272
5. Data show that Bcl-2 expression positively influences the targeting of nuclear-encoded COX Va and Vb to the mitochondria of cancer cells. PMID: 19834492
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亞細(xì)胞定位：

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
蛋白家族：

Cytochrome c oxidase subunit 5B family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 2269

OMIM: 123866

KEGG: hsa:1329

STRING: 9606.ENSP00000258424

UniGene: Hs.1342

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