CNOT3 Antibody

中文名稱：

CNOT3兔多克隆抗體
貨號：

CSB-PA005666LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA005666LA01HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA005666LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CNOT3 Polyclonal antibody
Uniprot No.：

O75175
基因名：

CNOT3
別名：

CCR4 associated factor 3 antibody; CCR4-associated factor 3 antibody; CCR4-NOT transcription complex subunit 3 antibody; CNOT3 antibody; CNOT3_HUMAN antibody; KIAA0691 antibody; LENG2 antibody; Leukocyte receptor cluster member 2 antibody; negative regulator of transcription 3, S. cerevisiae, homolog of antibody; NOT3 (negative regulator of transcription 3, yeast) homolog antibody; NOT3 antibody; NOT3H antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human CCR4-NOT transcription complex subunit 3 protein (257-395AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CNOT3 Antibody (CSB-PA005666LA01HU)，的標記方式是Non-conjugated。對于CNOT3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA005666LB01HU	CNOT3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA005666LC01HU	CNOT3 Antibody, FITC conjugated
Biotin	CSB-PA005666LD01HU	CNOT3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.
基因功能參考文獻：
1. Cnot3 enhances cardiomyocyte proliferation by promoting cell cycle inhibitor mRNA degradation. PMID: 28473716
2. our findings implicate CNOT3 in the coordination of colonic epithelial cell self-renewal, suggesting this factor as a new biomarker for molecular and prognostic classification of early-stage colorectal cancer PMID: 27899379
3. A mutation in PRPF31 is hypostatic to a trait acting on CNOT3, with the RP11 phenotype only being observed when there is homozygous (recessive) inheritance of the higher expressivity CNOT3 ("symptomatic" or risk) allele. PMID: 24116917
4. In asymptomatic carriers CNOT3 is expressed at low levels, allowing higher amounts of wild-type PRPF31 transcripts to be produced and preventing manifestation of retinal degeneration. PMID: 23144630
5. CNOT3 is a tumor suppressor mutated in 7 of 89 (7.9%) adult T-cell acute lymphoblastic leukemias, and its knockdown causes tumors in a sensitized Drosophila melanogaster model. PMID: 23263491
6. A high density SNP genotyping approach within the 19q13 chromosome region identifies an association of a CNOT3 polymorphism with ankylosing spondylitis. PMID: 22294640
7. CNOT3 depletion stabilizes the MAD1 mRNA, and MAD1 knockdown attenuates the CNOT3 depletion-induced increase of the mitotic index. PMID: 22342980
8. TBP-interacting protein 120B binds to NOT3 PMID: 12207886
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, P-body.
蛋白家族：

CNOT2/3/5 family
組織特異性：

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
數據庫鏈接：

HGNC: 7879

OMIM: 604910

KEGG: hsa:4849

STRING: 9606.ENSP00000221232

UniGene: Hs.343571

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