CDC27 Antibody

說明書

中文名稱：

CDC27兔多克隆抗體
貨號：

CSB-PA001504
規格：

￥1090
圖片：
Western Blot analysis of COLO205 cells using Cdc27 Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P30260
基因名：

CDC27
別名：

ANAPC3 antibody; Anaphase Promoting Complex 3 antibody; Anaphase promoting complex protein 3 antibody; Anaphase Promoting Complex Subunit 3 antibody; Anaphase-promoting complex subunit 3 antibody; APC 3 antibody; APC3 antibody; Cdc 27 antibody; Cdc27 antibody; CDC27 homolog antibody; CDC27_HUMAN antibody; CDC27Hs antibody; Cell Division Cycle 27 antibody; Cell division cycle protein 27 homolog antibody; D0S1430E antibody; D17S978E antibody; H NUC antibody; H-NUC antibody; HNUC antibody; Nuc2 homolog antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from the Internal region of Human Cdc27.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:5000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
基因功能參考文獻：
1. CDC27 facilitated cells invasion and metastasis via Twist pathway, leading to initiation of epithelial-mesenchymal transition and silencing of Twist expression could reverse this process. PMID: 30308498
2. mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 expression. PMID: 27974673
3. The levels of CDC20 and CylinB1 increased and the levels of Ku70 and APC3 decreased after irradiation. APC/C(Cdh1) is involved in regulation of radiosensitivity in human NPC CNE-1 cells. PMID: 28004426
4. Data indicate that UBE2S and APC3 co-regulate the expression level of P21 at G2/M check point via the ubiquitin-proteasome system. PMID: 28257844
5. Cdc27 is a novel binding partner of Elmo1.Cdc27-Elmo1 has a cellular role independent from the Elmo-Dock1-Rac signal module. PMID: 26882976
6. miR-27a could modulate proliferation and radiosensitivity of TNBC cells. CDC-27 is a direct target of miR-27a and its downregulation conferred increased radioresistance of the cells. PMID: 25943633
7. These findings suggested that polymorphisms in cdc27 may contribute to the susceptibility of BC though functional studies are needed to further elucidate the underling mechanisms of the associations PMID: 25680405
8. The structures show how one APC16 binds asymmetrically to the symmetric APC3 dimer and, together with biochemistry and prior data, explain how APC16 recruits APC7 to APC3. PMID: 25490258
9. Low cdc27 and high securin expression predict short survival for breast cancer patients. PMID: 23755904
10. Down-regulation of CDC27 protein is associated with radiation resistance. PMID: 23923534
11. Data indicate that additional density present in the anaphase-promoting complex (APC/C) structure, proximal to Apc3/Cdc27 of the (tetratricopeptide repeat) lobe, is assigned to the TPR subunit Apc7, a subunit specific to vertebrate APC/C. PMID: 23078409
12. the biochemical, structural, and cellular determinants of the novel interaction between MCPH1 and Cdc27 and suggest that this interaction may occur within the larger context of MCPH1-APC/C. PMID: 22139841
13. phosphorylation of Cdc27 by CKII is involved in TGF-beta-induced activation of APC. PMID: 21209074
14. the spindle checkpoint may regulate CDC27/APC activity at mitosis PMID: 12429948
15. HOXC10 co-immunoprecipitates the APC subunit CDC27, and its in vitro degradation is reduced in APC-depleted extracts or by competition with the APC substrate cyclin A. PMID: 12853486
16. The interaction is direct and is mediated by the tandem BRCA1 C-terminal domains of MDC1 and the C terminus of the Cdc27 (APC3) subunit of the anaphase-promoting complex/cyclosome. PMID: 17827148
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亞細胞定位：

Nucleus. Cytoplasm, cytoskeleton, spindle.
蛋白家族：

APC3/CDC27 family
數據庫鏈接：

HGNC: 1728

OMIM: 116946

KEGG: hsa:996

STRING: 9606.ENSP00000434614

UniGene: Hs.463295

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