CCNT1 Antibody
-
中文名稱：CCNT1兔多克隆抗體
-
貨號：CSB-PA940399
-
規格：￥1100
-
圖片：
-
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA940399(CCNT1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200) -
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA940399(CCNT1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: ×200)
-
-
其他：
產品詳情
-
Uniprot No.：
-
基因名：CCNT1
-
別名：CCN T1 antibody; CCNT antibody; CCNT 1 antibody; Ccnt1 antibody; CCNT1_HUMAN antibody; CDK9 associated C type protein antibody; Cyc T1 antibody; Cyclin C related protein antibody; Cyclin T antibody; cyclin T1 antibody; Cyclin T1b antibody; Cyclin-T antibody; Cyclin-T1 antibody; CYCT 1 antibody; CycT1 antibody; HIVE1 antibody; Human immunodeficiency virus 1 expression antibody; Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody; pTEFb subunit antibody; Subunit of positive elongation transcription factor b antibody
-
宿主：Rabbit
-
反應種屬：Human,Mouse
-
免疫原：Synthetic peptide of Human CCNT1
-
免疫原種屬：Homo sapiens (Human)
-
標記方式：Non-conjugated
-
抗體亞型：IgG
-
純化方式：Antigen affinity purification
-
濃度：It differs from different batches. Please contact us to confirm it.
-
保存緩沖液：-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
-
產品提供形式：Liquid
-
應用范圍：ELISA,IHC
-
推薦稀釋比：
Application Recommended Dilution ELISA 1:1000-1:2000 IHC 1:25-1:100 -
Protocols：
-
儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
-
功能：Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).; (Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
-
基因功能參考文獻：
- ASF/SF2 is identified as a splicing regulator of cyclin T1, which contributes to the control of the subsequent transcription events. PMID: 28422315
- The TAR central loop contacts the CycT1 Tat-TAR recognition motif (TRM) and the second Tat Zn(2+)-binding loop. Hydrogen-deuterium exchange (HDX) shows that AFF4 helix 2 is stabilized in the TAR complex despite not touching the RNA, explaining how it enhances TAR binding to the SEC 50-fold. PMID: 27731797
- Quantitative measurement of the molecular interactions among Tat, CycT1 and CDK9 has showed that any third molecule enhances the binding between the other two molecules. These findings suggest that each component of the Tat:P-TEFb complex stabilizes the overall complex, thereby supporting the efficient transcriptional elongation during viral RNA synthesis. PMID: 27193293
- the introduction of HIV-1 Tat and Mg12+ ion resulted in the active site architectural characteristics of phosphorylated CDK9/cyclin T1 complex PMID: 25909811
- AFF4 is positioned to make unexpected direct contacts with HIV Tat, and Tat enhances P-TEFb/CCNT1 affinity for AFF4. PMID: 23471103
- Plk1 negatively regulates the RNA polymerase II-dependent transcription through inhibiting the activity of cyclin T1/Cdk9 complex. PMID: 23977272
- The sequence domain of human cytomegalovirus pUL97 responsible for the interaction with cyclin T1 was between amino acids 231-280. PMID: 24351800
- the release of P-TEFb from the 7SK snRNP led to increased synthesis of HEXIM1 but not HEXIM2 PMID: 24515107
- All of the hCD4/R5/cT1 mice developed disseminated infection of tissues that included the spleen, small intestine, lymph nodes and lungs after intravenous injection with an HIV-1 infectious molecular clone PMID: 23691059
- positive transcription elongation factor b (P-TEFb, made of CDK9 and CycT1) is released from its inhibitory complex by histone deacetylase inhibitors, which also activate HIV transcription PMID: 23539624
- CYCT1b is a newly identified cyclin T1 splice variant whose expression leads to direct inhibition of TAT-transactivated transcription of the HIV-1 LTR promoter PMID: 23569210
- Structural determination of a short-lived conformational state between CCNT1 and CDK9 that is adopted during the phosphorylation cycle. PMID: 22959624
- The authors find that resting CD4(+) T cells, whether naive or memory and independent of their HIV infection status, have low levels of Cyclin T1 and T-loop-phosphorylated CDK9, which increase upon activation. PMID: 23152527
- N-terminal residues in cyclin T1 are specifically involved in the binding of cyclin T1 to HEXIM1 but not to Tat. PMID: 22342181
- MiR-27b, miR-29b, miR-223, and miR-150 are regulators of cyclin T1 protein levels and HIV-1 replication in resting CD4+ T cells. PMID: 22205749
- The role of the CycT1 N-terminal region in Tat action, was investigated. PMID: 21763494
- This investigation reveals a novel mechanism of cyclin T1 regulation and establishes NF90 as a regulator of HIV-1 replication during both productive infection and induction from latency. PMID: 21763496
- Cyclin K inhibits HIV-1 gene expression and replication by interfering with cyclin-dependent kinase 9 (CDK9)-cyclin T1 interaction in Nef-dependent manner. PMID: 21555514
- HCMV inter-regulation with CDK9/cyclin T1 is at least partly based on a pUL69-cylin T1 interaction. PMID: 21450947
- study showed that P-TEFb and Dengue virus (DENV) core protein work in concert to enhance IL-8 gene expression by DENV infection PMID: 20618343
- results suggest that cyclin T1/CDK9 serves as an adapter to mediate the interaction of influenza A virus RNA-dependent RNA polymerase and RNA Pol II and promote viral transcription PMID: 20943989
- Cyclin T1 oncogenic function and suggest a role for this protein in controlling cell cycle probably via Rb/E2F1 pathway. PMID: 20714219
- binding to Cyclin T1 induces an asymmetry or sterical hindrance in the first coiled coil segment of dimeric Hexim1 that disallows formation of a 2:2 complex. PMID: 20210365
- Activity inhibited by 7SK small nuclear RNA. PMID: 11713533
- P-TEFb containing cyclin K and Cdk9 can activate transcription via RNA. PMID: 11884399
- CDK9 has the intrinsic property to shuttle between nucleus and cytoplasm, and enhanced expression of cyclin T1 promotes its nuclear localization. PMID: 12115727
- P-TEFb is a key mediator of Myc activated transcription by stimulating elongation PMID: 12177005
- fluctuation in CycT1 levels during human macrophage differentiation may be involved in the regulation of HIV-1 replication PMID: 12368300
- chimeras between kinase-inactive mutant Cdk9 and truncated cyclin T1 proteins efficiently inhibit Tat transactivation and human immunodeficiency virus gene expression PMID: 12368330
- Tat and trans-activation-responsive (TAR) RNA-independent induction of HIV-1 long terminal repeat by this protein requires Sp1 PMID: 12458222
- Cyclin T1 binding to HIV-1 tat is regulated by the differential acetylation of tat PMID: 12486002
- cyclin T1 binds with granulin to inhibit transcription PMID: 12588988
- the transcriptional repressor PIE-1 from Caenorhabditis elegans binds Cyclin T1 via an alanine-containing heptapeptide repeat and inhibits transcriptional elongation {cyclin T1) PMID: 12651893
- recruitment to nuclear bodies via direct interaction with PML protein PMID: 12727882
- Since Tat also binds to this cyclin T1 domain and the association between 7SK RNA/MAQ1 and P-TEFb competes with the binding of Tat to cyclin T1, we speculate that the TAR RNA/Tat lentivirus system has evolved to subvert the cellular 7SK RNA/MAQ1 system PMID: 12832472
- major portion of nuclear P-TEFb is sequestered and inactivated by the coordinated actions of the 7SK snRNA PMID: 14627702
- RNAi-mediated gene silencing of P-TEFb in HeLa cells was not lethal and inhibited Tat transactivation and HIV-1 replication in host cells PMID: 14963154
- review of work indicating under some circumstances TAK/P-TEFb is likely to be limiting for HIV replication in CD4+ T cells and macrophages; review of mechanisms of regulation of the TAK/P-TEFb subunits in these cell types PMID: 15049426
- NUFIP and P-TEFb with BRCA1 activate transcription by RNA polymerase II PMID: 15107825
- a histidine-rich stretch in CycT1 was found to direct the transcriptional activity of this P-TEFb complex when tethered artificially to DNA. PMID: 15563843
- HEXIM1 and HEXIM2 maintain the balance between active and inactive forms of P-TEFb, a heterodimeric complex composed of cyclin-dependent kinase 9 and cyclin T. PMID: 15713661
- downregulation of hCycT1 did not cause apoptotic cell death PMID: 15913611
- increased estrogen down regulated gene 1 expression results in inhibition of cyclin T1 recruitment and estrogen receptor 1 DNA binding PMID: 15940264
- analysis of positive transcription elongation factor - HEXIM1 - 7SK RNA complex PMID: 15994294
- Role of P-TEFb as an activator of transcription elongation can be separated from its role in RNA processing; neither function is universally required for expression of mammalian pol II-dependent genes. PMID: 16308568
- the interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct its binding to P-TEFb and subcellular localization that culminates in the inhibition of transcription PMID: 16362050
- human cyclin T1 expression is sufficient to support Tat-mediated transactivation in primary mouse CD4 T lymphocytes and monocytes/macrophages and increases in vitro and in vivo HIV-1 production by these stimulated cells. PMID: 16439541
- Using DNA microarray technology, we found that more than 20% of genes induced by PMA require cyclin T1 for their normal level of induction, and approximately 15% of genes repressed by PMA require cyclin T1 for their normal level of repression. PMID: 16764723
- These data link the P-TEFb equilibrium to the intracellular transcriptional demand and proliferative/differentiated states of cells. PMID: 16980611
- Prostratin induces Cyclin T1 expression and P-TEFb function and this is likely to be involved in prostratin reactivation of latent HIV-1 proviruses PMID: 17014716
顯示更多
收起更多
-
亞細胞定位：Nucleus.
-
蛋白家族：Cyclin family, Cyclin C subfamily
-
組織特異性：Ubiquitously expressed.
-
數據庫鏈接：
Most popular with customers
-
-
YWHAB Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC, IF, FC
Species Reactivity: Human, Mouse, Rat
-
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC
Species Reactivity: Human
-
-
-
-
-
