CCND3 Antibody
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中文名稱：CCND3兔多克隆抗體
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貨號：CSB-PA004814ESR2HU
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規格：￥3900
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圖片：
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Western Blot
Positive WB detected in: K562 whole cell lysate(20μg), JK whole cell lysate(20μg), Raji whole cell lysate(20μg), Hela whole cell lysate(20μg), A549 whole cell lysate(20μg)
All lanes:CCND3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 33,23,25,10 kDa
Observed band size: 33 kDa
Exposure time: 120s -
Immunofluorescence staining of Hela cell with CSB-PA004814ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of A549 cell with CSB-PA004814ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) CCND3 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：CCND 3 antibody; Ccnd3 antibody; CCND3_HUMAN antibody; CyclinD3 antibody; D3 type cyclin antibody; G1 S specific cyclin D3 antibody; G1/S specific cyclin D3 antibody; G1/S-specific cyclin-D3 antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human G1/S-specific cyclin-D3 protein (248-292AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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產品提供形式：Liquid
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應用范圍：ELISA, WB,IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:20-1:100 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4.
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基因功能參考文獻：
- The Novel Short Isoform of Securin Stimulates the Expression of Cyclin D3 and Angiogenesis Factors VEGFA and FGF2, but Does Not Affect the Expression of MYC Transcription Factor PMID: 29989583
- miR-212 exerts growth-suppressive effects in Adult T-cell leukemia/lymphoma (ATL) cells largely by targeting CCND3 and may have therapeutic potential in ATL. PMID: 27493231
- Cyclin D3 is expressed in the majority of splenic diffuse red pulp small B-cell lymphomas. Increased expression is sometimes the result of somatic mutations in the PEST domain of the CCND3 gene. PMID: 28069605
- in ovarian cancer cells, DOT1L regulates the transcription of G1 phase genes CDK6 and CCND3 through H3K79 dimethylation PMID: 28114995
- metabolic function of cyclin D3-CDK6 kinase in cancer cell survival PMID: 28607489
- we showed that ZNF224 positively modulates cyclin D3 gene expression. Consistently, we observed that alteration of ZNF224 expression leads to defects in cell cycle control. All together, our results strongly suggest that in Chronic lymphocytic leukaemia (CLL)cells high expression level of ZNF224 can lead to inappropriate cell growth and apoptosis resistance, thus contributing to CLL progression PMID: 28040726
- the activation of TLR7 increased CCND3 expression via the downregulation of miR-15b in B cells. PMID: 26144250
- This study describes the identification and characterization of cyclin D3 as a novel interactor of influenza A virus M2 protein. PMID: 28130444
- MicroRNA-138 interacts with cyclin D3 and negatively regulates non-small cell lung cancer cells PMID: 26201895
- Combined urinary FGFR3/Cyclin D3 expression shows improved detection rates for bladder cancer recurrence with high specificity and sensitivity. PMID: 26861974
- The CDK6-cyclin D3 pair play a fundamental role in controlling CDK2-dependent SAMHD1 phosphorylation and the dNTP pool in primary macrophages. PMID: 25927932
- Two recurrent fusion genes associated with the 12q locus, LRP1-SNRNP25 and KCNMB4-CCND3, were by RT-PCR, Sanger sequencing and FISH, and were found to be osteosarcoma specific in a validation cohort of 240 other sarcomas. PMID: 25300797
- G1 arrest induced by SB265610 occurred at concentrations lacking CXCR2 selectivity and revealed cyclin-dependent kinase 2 (CDK2) (Thr160) hypophosphorylation, cyclin D3 gene down-regulation, and p21 post-translational induction PMID: 26026083
- sLZIP regulates the transcription of cyclin D3 by binding directly to the AP-1 region in the cyclin D3 promoter. PMID: 24441043
- combined expression of miR-138 and its direct target CCND3 may be correlated with significant characteristics of hepatocellular carcinoma PMID: 25439221
- Namely the amplification of the expression of the PLCB1a, but not of PLCB1b, is able to maintain high levels of expression of cyclin D3 even after treatment with kinamycin F PMID: 25160985
- cell cycle related proteins PCNA, Ki67, cyclin D3, p27 and p57 were expressed in both normal and diabetic human term placentas. PMID: 23963898
- two cell cycle-related molecules, cyclin D3 and E2F3, were identified as the direct miR-503 targets. PMID: 23967867
- PCNSL prognosis is relatively poor. Age, high tumor burden, higher lymphocyte count, expression of Cyclin D3, and Cyclin E are inferior prognostic factors for PCNSL. PMID: 23422111
- CCND3 gene amplification is a marker of aggressiveness and might be a predictor of tumor progression in bladder urothelial carcinoma PMID: 23830405
- Study reports that a cis-acting element, located between nucleotides 31 and 50 of the human CCND3 5' UTR, forms a stable G-quadruplex structure and represses translation of a reporter gene and the CCND3 gene in human cell lines. PMID: 22858673
- Lycorine hydrochloride effectively inhibited mitotic proliferation of Hey1B cells through enhanced expression of the cell cycle inhibitor p21 and marked down-regulation of cyclin D3 expression. PMID: 23376478
- No significant correlation was found between p-27, Cyclin D3, and cyclin E in pediatric Embryonal tumors PMID: 22763761
- 7 gains of CCND3 were seen amon 17 orbital adnexa MALT lymphomas. CCND3 might simply be a marker of 6p gains and not play a major role in the pathogenesis of MALT lymphomas. PMID: 23240690
- cyclin D3 is reduced in myotonic dystrophy due to increased phosphorylation at T283 by GSK3beta, which triggers the degradation of cyclin D3. PMID: 23160194
- CCND3 protein expression was observed to be negatively correlated with miR-138 expression in HCC tissues. PMID: 22362728
- The overexpression of ETS1 could suppress cyclin D3 mRNA and protein levels. PMID: 21841808
- Lymph node sections from 138 HL patients were immunohistochemically stained for cyclin D3 (CCND3), MCM2 and MCM7 aiming to investigate clinical outcome. PMID: 21965782
- These results suggest that alpha9-nAChR-mediated cyclin D3 overexpression is important for nicotine-induced transformation of normal human breast epithelial cells. PMID: 20229177
- In addition to having a pivotal role in the up-regulation of IL-2 and IL-2RA gene expression, IKK controls the expression of cyclin D3, cyclin E and CDK2, and the stability SKP2 and its co-factor CKS1B, through mechanisms independent of IL-2. PMID: 20465575
- Overexpressions of nuclear cyclin D3 is associated with non-small cell lung cancer. PMID: 20631637
- study suggests that Cyclin D3 gene amplification might be a predictor of aggressiveness in BCG-treated bladder urothelial carcinoma in situ PMID: 20821231
- Gene knockdown of cyclin D3 did not inhibit pRb phosphorylation on cdk4/6- and cdk2-specific residues or measurably affect viability and proliferation. PMID: 20107311
- Ubiquitin/proteasome-dependent degradation of D-type cyclins is linked to tumor necrosis factor-induced cell cycle arrest. PMID: 11864973
- High cyclin D3 expression had a significantly lower response to antineoplastic agents in diffuse large B-cell lymphomas PMID: 11895902
- interaction with p58(PITSLRE) PMID: 12082095
- A cofactor of retinoic acid receptors, modulating their activity in the presence of cellular retinoic acid-binding protein II. PMID: 12482873
- cyclin D3 is activated by E2F1;the essential E2F regulatory element of the cyclin D3 promoter is between nucleotides -143 and -135 relative to the initiating methionine codon PMID: 12611887
- overexpression of cyclin D3 was mutually exclusive with Rb/p16 aberrant expression status supporting an oncogenic role for cyclin D3 PMID: 12647795
- High levels of this protein are gound in malignant glioma. PMID: 12778072
- cyclin D3 protein is expressed in a fraction of human goiters but it is strongly overexpressed in most follicular adenomas PMID: 14576819
- cyclin d3 does not have a role in regulating AML1/RUNX1 increase during G1 to S cell cycle progression PMID: 14747476
- GSK-3beta has a role in cAMP-induced degradation of cyclin D3 PMID: 15252116
- Increased expression of Cyclin D3 is associated with follicular lymphoma PMID: 15305377
- cyclin D3 is degraded via proteasome and that Thr-283 is essential for its degradation PMID: 15326477
- cyclin D3 specifically interacted with eIF3k through its C-terminal domain; eIF3k distributed both in nucleus and cytoplasm and colocalized with cyclin D3 PMID: 15327989
- activating transcription factor 5 (ATF5) is a new interacting partner of cyclin D3 PMID: 15358120
- silencing cyclin D3 by RNA interference inhibits S phase entry and sensitizes breast cancer cells to TRAIL, indicating a key role for cyclin D3 repression in these events PMID: 15569667
- cyclin D3 may have a role in progression of laryngeal squamous cell carcinoma PMID: 15671552
- Cyclin D3 up-regulated transcriptional activity of VDR and this effect was counteracted by overexpression of CDK4 and CDK6 PMID: 16105657
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亞細胞定位：Nucleus. Cytoplasm.
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蛋白家族：Cyclin family, Cyclin D subfamily
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數據庫鏈接：
