CBFA2T3 Antibody

中文名稱：

CBFA2T3兔多克隆抗體
貨號：

CSB-PA004576LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Jurkat whole cell lysate, 293T whole cell lysate, HL60 whole cell lysate
All lanes: CBFA2T3 antibody at 5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72, 63, 64, 8 kDa
Observed band size: 72 kDa

IHC image of CSB-PA004576LA01HU diluted at 1:300 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CBFA2T3 Polyclonal antibody
Uniprot No.：

O75081
基因名：

CBFA2T3
別名：

Cbfa2t3 antibody; Ccore binding factor runt domain alpha subunit 2 translocated to 3 antibody; ETO2 antibody; hMTG16 antibody; hMTG16a antibody; hMTG16b antibody; MTG16 antibody; MTG16_HUMAN antibody; MTG8 related gene 2 antibody; MTG8 related protein MTG16a antibody; MTG8 related protein MTG16b antibody; MTG8-related protein 2 antibody; MTGR2 antibody; Myeloid translocation gene 8 and 16b antibody; Myeloid translocation gene on chromosome 16 protein antibody; Protein CBFA2T3 antibody; Zinc finger MYND domain-containing protein 4 antibody; ZMYND4 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Protein CBFA2T3 protein (39-159AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CBFA2T3 Antibody (CSB-PA004576LA01HU)，的標記方式是Non-conjugated。對于CBFA2T3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA004576LB01HU	CBFA2T3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA004576LC01HU	CBFA2T3 Antibody, FITC conjugated
Biotin	CSB-PA004576LD01HU	CBFA2T3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation.; Isoform 2 functions as an A-kinase-anchoring protein.
基因功能參考文獻：
1. Analysis of chromatin immunoprecipitation sequencing data sets for MTGR1 and MTG16 targets indicated that MTGR1 can regulate Wnt and Notch signaling. PMID: 27270437
2. CBFA2T3 expression is regulated by Med19 in breast cancer cells. PMID: 27572702
3. specific interference with ETO2-GLIS2 oligomerization reverses the transcriptional activation at enhancers and promotes megakaryocytic differentiation, providing a relevant interface to target in this poor-prognosis pediatric leukemia. PMID: 28292442
4. Clinical courses of pediatric patients with AMKL harboring the CBFA2T3-GLIS2 fusion gene are poor due to resistance to chemotherapies and SCT. New treatment strategies are necessary. PMID: 27094503
5. ETO2 and IRF2BP2 interacting with the NCOR1/SMRT co-repressor complex, suppresses the expression of erythroid genes until erythroid differentiation. PMID: 26593974
6. MTG16 co-repressor promotes degradation of HIF1alpha in lymphoblasts. PMID: 25974097
7. study defined eight additional recurrently mutated genes in SMZL; these genes are CREBBP, CBFA2T3, AMOTL1, FAT4, FBXO11, PLA2G4D, TRRAP and USH2A. PMID: 24349473
8. Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML. PMID: 24127550
9. Expression of MTG16 reduced glycolytic metabolism while mitochondrial respiration and formation of reactive oxygen species increased. PMID: 23840896
10. presence of MTG16 in this complex, and its contributions to transcriptional repression both required Kaiso binding to its binding site on DNA, establishing MTG16-Kaiso binding as functionally relevant in Kaiso-dependent transcriptional repression PMID: 23251453
11. Data indicate taht ETO2/CBFA2T3 regulates GATA-1 target genes critical for erythroid differentiation, and the decrease of ETO2 levels during erythroid differentiation would contribute to the activation of these targets. PMID: 23127762
12. Identification of a cryptic chromosome 16 inversion (inv(16)(p13.3q24.3)) in 27% of pediatric acute megakaryoblastic leukemia cases, which encodes a CBFA2T3-GLIS2 fusion protein. PMID: 23153540
13. An evolutionary conserved GATA binding site is critical in transcriptional regulation of the MTG16 promoter. PMID: 22443175
14. Our data indicate that the CBFA2T3/ACSF3 locus is a novel recurrent oncogenic target of immunoglobulin heavy chain translocations, which might contribute to the pathogenesis of pediatric GC-derived B-cell lymphoma. PMID: 22420028
15. Alternative NLI complexes mediate gamma-globin transcription or silencing through long-range locus control region interactions involving an intergenic site of noncoding RNA transcription and that ETO2 is critical to this process. PMID: 22010104
16. Study provides the first evidence that MTG16b is a dual AKAP capable of binding plexins. PMID: 20138877
17. Data show that ZNF651 is a ZNF652 paralogue that shares a common DNA binding sequence with ZNF652 and represses target gene expression through the formation of a CBFA2T3-ZNF651 corepressor complex. PMID: 20116376
18. In the first pediatric leukemia case in whom AML1-MTG16 was identified, the breakpoint of MTG16 was determined, and suggested as a possible characteristic of secondary leukemia. PMID: 11999578
19. CBFA2T3 (MTG16) is a putative breast tumor suppressor gene from the breast cancer loss of heterozygosity region at 16q24.3. PMID: 12183414
20. The transcriptional repression of growth factor independent-1 (GFI-1), a previously characterized ETO effector zinc finger protein, was shown to be enhanced by CBFA2T1, but to a lesser extent by CBFA2T2 and CBFA2T3. PMID: 16966434
21. CBFA2T3 interacts with ZNF652 to repress HEB expression, and in addition CBFA2T3 interacts with the HEB protein to inhibit its activator function. PMID: 18456661
22. RUNX1-MTG16 fusion gene in acute myeloblastic leukemia with t(16/21)(q24'q22) is reported. PMID: 18656694
23. Both wild type MTGs and chimeric AML1-MTG proteins display in vitro RNA-binding properties PMID: 18950503
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相關疾?。?/div>
A chromosomal aberration involving CBFA2T3 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
亞細胞定位：

[Isoform 1]: Nucleus, nucleolus. Note=The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm.; [Isoform 2]: Nucleus, nucleoplasm. Golgi apparatus membrane.
蛋白家族：

CBFA2T family
組織特異性：

Widely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).
數據庫鏈接：

HGNC: 1537

OMIM: 603870

KEGG: hsa:863

STRING: 9606.ENSP00000268679

UniGene: Hs.513811

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