CARM1 Antibody

中文名稱：

CARM1兔多克隆抗體
貨號：

CSB-PA771462LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, NIH/3T3 whole cell lysate, Jurkat whole cell lysate, Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue
All lanes: CARM1 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 64, 42 kDa
Observed band size: 66, 42 kDa

Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA771462LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA771462LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CARM1 Polyclonal antibody
Uniprot No.：

Q86X55
基因名：

CARM1
別名：

carm1 antibody; CARM1_HUMAN antibody; Coactivator associated arginine methyltransferase 1 antibody; Coactivator-associated arginine methyltransferase 1 antibody; Histone arginine methyltransferase CARM 1 antibody; Histone arginine methyltransferase CARM1 antibody; Histone-arginine methyltransferase CARM1 antibody; PRMT 4 antibody; PRMT4 antibody; Protein arginine methyltransferase antibody; Protein arginine N methyltransferase 4 antibody; Protein arginine N-methyltransferase 4 antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Histone-arginine methyltransferase CARM1 protein (209-379AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CARM1 Antibody (CSB-PA771462LA01HU)，的標記方式是Non-conjugated。對于CARM1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA771462LB01HU	CARM1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA771462LC01HU	CARM1 Antibody, FITC conjugated
Biotin	CSB-PA771462LD01HU	CARM1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:2000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
基因功能參考文獻：
1. N-terminal EVH1 domain of CARM1 is necessary and sufficient for substrate recognition and is required for methylation of most CARM1 substrates. PMID: 28537268
2. SKP2 promotes HCC progression and its nuclear functions of autophagy induction with CARM1 and AMPK, which may provide a potential target for HCC therapy. PMID: 29991055
3. The Overexpression of CARM1 Promotes Human Osteosarcoma Cell Proliferation through the pGSK3beta/beta-Catenin/cyclinD1 Signaling Pathway PMID: 28924379
4. The CARM1-PKM2 axis serves as a metabolic reprogramming mechanism in tumorigenesis. PMID: 29058718
5. Arginine (di)methylated human leukocyte antigen class I peptides, which are asymmetrically dimethylated, most likely by CARM1, are favorably presented by HLA-B*07. PMID: 27503676
6. Estrogen receptor recruits steroid receptor coactivator-3 primary coactivator and secondary coactivators, p300/CBP and CARM1 to regulate genetic transcription. PMID: 28844863
7. Arginine methylation of MDH1 by CARM1 regulates cellular redox homeostasis and suppresses glutamine metabolism of pancreatic cancer. PMID: 27840030
8. Here, the crystal structures of human CARM1 with the S-adenosylmethione (SAM) mimic sinefungin and three different peptide sequences from histone H3 and PABP1 are presented, with both nonmethylated and singly methylated arginine residues exemplified. PMID: 26551522
9. CARM1 associates with major nonsense-mediated mRNA decay factor UPF1 and promotes its occupancy on premature terminating codon-containing transcripts in spinal muscular atrophy. PMID: 26656492
10. Monitoring of the CARM1-dependent production of monomethylated and dimethylated peptides over time by self-assembled monolayer and matrix-assisted laser desorption ionization mass spectrometry revealed that methylation by CARM1 is distributive. PMID: 26848779
11. no obvious association of CARM1 isoform expression and clinical correlates in breast cancer PMID: 26030442
12. disruption of CARM1 enhances the nuclear retention of mRNAs containing IRAlus PMID: 25792598
13. Data indicate that coactivator-associated arginine methyltransferase 1 (CARM1) regulates neural differentiation through Nanog homeobox protein and microRNA miR92a. PMID: 25392304
14. Like insulin, CARM1 overexpression increased CDK2 and CDK4 expression. In addition, CARM1 knockdown reduced the number of insulin-induced G2/M phase cells PMID: 25934150
15. a noncoding variant in the CARM1-promoter functions as a regulator of gene transcription and homocysteine levels PMID: 25064859
16. O-GlcNAcylation of CARM1 at its C-terminus is an important determinant for CARM1 substrate specificity. PMID: 25585345
17. PRMT4 has a role in arginine methylation, which negatively regulates retinoblastoma tumor suppressor protein and promotes E2F-1 dissociation PMID: 25348716
18. PBMCs from patients with ACS showed higher levels of CARM1 mRNA and protein expression.CARM1 regulated the transcription of IP-10, IL-8, and MCP-1. miR-15a modulated CARM1 expression through targeted binding to CARM1 3'-UTR. PMID: 24530761
19. high-glucose-induced CARM1 expression increases RPE cell apoptosis via H3R17 asymmetric dimethylation PMID: 25072916
20. CARM1 and PRMT1 are dysregulated in lung cancer; only CARM1 expression was found to be correlated with tumor differentiation and neither CARM1 nor PRMT1 expression was correlated with survival. PMID: 24211191
21. Overexpression of PRMT4 represses miR-223 via methylation of RUNX1 and blocks myeloid differentiation. PMID: 24332853
22. CARM1 overexpression was associated with poorly characterized clinicopathologic parameters and HER2 overexpression in invasive breast cancer. PMID: 23915145
23. we identify BAF155 as a substrate for arginine methyltransferase CARM1. PMID: 24434208
24. increased cytoplasmic CARM1, relative to nuclear levels, appear to be associated with self-identified African ethnicity and this result is being further investigated using quantified genetic ancestry measures. PMID: 23663560
25. CARM1 could be a potential marker of luminal class subclassification and for target therapy, particularly in the ER-positive luminal-like subgroup. PMID: 23887673
26. Arginine 42 of histone H3 (H3R42), is dimethylated by the methyltransferases coactivator arginine methyltransferase 1 (CARM1) and protein arginine methyltransferase 6 (PRMT6) in vitro and in vivo. PMID: 23980157
27. Data show the transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. PMID: 23699174
28. CARM1 represses replicative senescence by methylating HuR. PMID: 23837869
29. Inhibition of CARM1 decreases PELP1 oncogenic activity. PMID: 23486015
30. the miR-181c-CARM1 pathway has an important role in regulating the differentiation of human embryonic stem cells PMID: 23301034
31. These findings reveal an important and so far unknown connection between PRMT4 and the chromatin remodeller Mi2 in c-Myb signalling. PMID: 23505388
32. Findings indicate that expression of PRMT4/CARM1 and PRMT6 is deregulated in melanoma. PMID: 23265702
33. found that CARM1 facilitates Sox2-mediated transactivation and directly methylates Sox2 at arginine 113 PMID: 22046437
34. Signal transduction in cell death and survival involves a role for CARM1 in arginine methylation. PMID: 21445011
35. A coactivator role of CARM1 in the dysregulation of beta-catenin activity in colorectal cancer cell growth and gene expression PMID: 21478268
36. Findings suggest that coexpression of CARM1 and ERalpha may provide a better biomarker of well-differentiated breast cancer. PMID: 21282336
37. CARM1 was particularly overexpressed in colorectal cancers while CARM1 expression was not prevalent in prostate and breast cancers. PMID: 20462455
38. The physical interaction of CARM1 and PCAF is likely pivotal for the activation of PCAF in the downstream of CARM1 pathway for inducing myogenin under Tetradecanoylphorbol Acetate -induced differentiation. PMID: 20213728
39. phosphorylation of CARM1 by PKA at a single serine is necessary and sufficient for direct binding to the unliganded hormone-binding domain of ERalpha, and the interaction is necessary for cAMP activation of ERalpha PMID: 20360387
40. Methylates PAB1 and TARPP, suggesting a role in regulating transcription/translation. PMID: 11850402
41. The CBP/p300 acetylase and the CARM1 methyltransferase can positively regulate the expression of estrogen-responsive genes, there is a crosstalk between lysine acetylation and arginine methylation on chromatin PMID: 12498683
42. CARM1 plays a significant role in promoting the differentiation of early thymocyte progenitors, possibly through its direct action on TARPP. PMID: 15096520
43. overexpression of CARM1 is involved in the development of prostate carcinoma PMID: 15221992
44. results show that CARM1/PRMT4 is a novel transcriptional coactivator of NF-kappaB and functions as a promoter-specific regulator of NF-kappaB recruitment to chromatin PMID: 15616592
45. CARM1 affects pre-mRNA splicing in an isoform-specific manner PMID: 15944154
46. arginine methylation of CBP is required for IFN-gamma induction of MHC-II. A kinetic analysis shows that CIITA, CARM1, and H3-R17 methylation all precede CBP loading on the MHC-II promoter during IFN-gamma treatment. PMID: 16254053
47. CARM1 has a role in signal-dependent control of gluconeogenic key enzyme genes PMID: 16330542
48. CARM1 participates in NF-kappaB-mediated transcription through H3-R17 methylation and support a nonnuclear receptor-associated function for CARM1. PMID: 16497732
49. CARM1 is essential for androgen receptor (AR) function and may play a role in prostate cancer progression. PMID: 16705743
50. Results suggest that members of the SRC coactivator family, such as SRC-3, serve as substrates for the enzymatic coactivator coactivator-associated arginine methyltransferase 1 (CARM1). PMID: 16923966
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亞細胞定位：

Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
蛋白家族：

Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
組織特異性：

Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
數據庫鏈接：

HGNC: 23393

OMIM: 603934

KEGG: hsa:10498

STRING: 9606.ENSP00000325690

UniGene: Hs.323213

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