CAND1 Antibody

中文名稱：

CAND1兔多克隆抗體
貨號：

CSB-PA774815LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate
All lanes: CAND1 antibody at 4.9µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 137, 118, 46 kDa
Observed band size: 137 kDa

IHC image of CSB-PA774815LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA774815LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA774815LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CAND1 Polyclonal antibody
Uniprot No.：

Q86VP6
基因名：

CAND1
別名：

CAND 1 antibody; Cand1 antibody; CAND1_HUMAN antibody; Cullin associated and neddylation dissociated 1 antibody; Cullin associated and neddylation dissociated protein 1 antibody; Cullin associated NEDD8 dissociated protein 1 antibody; Cullin-associated and neddylation-dissociated protein 1 antibody; Cullin-associated NEDD8-dissociated protein 1 antibody; DKFZP434M1414 antibody; FLJ10114 antibody; FLJ10929 antibody; FLJ38691 antibody; FLJ90441 antibody; KIAA0829 antibody; p120 CAND1 antibody; TATA binding protein interacting protein 120A antibody; TBP interacting protein antibody; TBP interacting protein of 120 kDa A antibody; TBP Interacting Protein TIP120A antibody; TBP-interacting protein 120A antibody; TBP-interacting protein of 120 kDa A antibody; TIP120 antibody; TIP120 protein antibody; TIP120A antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Cullin-associated NEDD8-dissociated protein 1 protein (1150-1230AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CAND1 Antibody (CSB-PA774815LA01HU)，的標記方式是Non-conjugated。對于CAND1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA774815LB01HU	CAND1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA774815LC01HU	CAND1 Antibody, FITC conjugated
Biotin	CSB-PA774815LD01HU	CAND1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.
基因功能參考文獻：
1. miR-33a and CAND1 played an important role in lung cancer proliferation and cell migration PMID: 28871425
2. describe the identification of the structural determinants responsible for the CA IX/CAND1 interaction PMID: 28388044
3. Data show that the differentiation of LiSa-2 preadipocytes is associated with an increase of cullin-associated and neddylation-dissociated 1 (CAND1), COP9 signalosome (CSN), neddylated cullin 3 (Cul3) and the BTB protein Keap1. PMID: 26219975
4. The Epstein-Barr virus protein BPLF1, is targeted to cullin-RING ubiquitin ligases (CRLs) via the interaction of the conserved helix-2 with helix-23 of cullins, at a site involved in electrostatic interaction with CAND1. PMID: 22474075
5. We demonstrate that the accumulation of p27 is associated with an increase of CAND1 and a decrease of Skp2 during adipogenesis of human LiSa-2 preadipocytes. CAND1 knockdown reduces p27 and blocks adipogenesis. PMID: 23328082
6. Study shows that Cand1 can unambiguously stimulate SCF activity in vitro by enabling an F box protein-Skp1 complex to access Cul1 that was previously occupied by a different F box protein-Skp1 complex, and that Cand1 promotes assembly in vivo of new F box proteins with pre-existing Cul1 molecules. PMID: 23453757
7. CAND1 promotes PLK4-mediated centriole overduplication and is frequently disrupted in prostate cancer. PMID: 23019411
8. COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding. PMID: 21778237
9. CAND1 does not function by sequestering cullins in vivo to prevent substrate receptor autoubiquitination and is likely to regulate cullin RING ligase activity via alternative mechanisms PMID: 21249194
10. miR-148a is an androgen-responsive microRNA that promotes LNCaP prostate cell growth by repressing its target CAND1 expression. PMID: 20820187
11. selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation PMID: 12504025
12. binds to unneddylated CUL1 and regulates the formation of SCF ubiquitin E3 ligase complex PMID: 12504026
13. TIP120A functions as a negative regulator of SCF E3 ubiquitin ligases and may modulate other cullin ligases in a similar fashion. PMID: 12609982
14. CAND1 & COP9 signalosome (CSN), major deneddylase of cullins, bind to unneddylated CUL1 in mutually exclusive way. Suppression of CAND1 expression by siRNA enhanced interaction between CUL1 & CSN, suggesting that CAND1 inhibited binding of CSN to CUL1. PMID: 16036220
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亞細胞定位：

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic.
蛋白家族：

CAND family
數據庫鏈接：

HGNC: 30688

OMIM: 607727

KEGG: hsa:55832

STRING: 9606.ENSP00000299218

UniGene: Hs.546407

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