BTN3A1 Antibody

中文名稱：

BTN3A1兔多克隆抗體
貨號：

CSB-PA002873LA01HU
規格：

￥3900
圖片：
Western Blot
Positive WB detected in: U937 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
All lanes: BTN3A1 antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40-58 kDa
Observed band size: 48 kDa

Immunofluorescence staining of Hela cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SH-SY5Y cell with CSB-PA002873LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品描述：

To generate the BTN3A1 polyclonal antibody, rabbits were immunized with recombinant human BTN3A1 (30-254aa). The antibody was then purified using Protein G to achieve a purity level of over 95%, ensuring its high quality and freedom from impurities that may affect experimental outcomes. This antibody is capable of detecting the BTN3A1 protein in both human and mouse samples, making it a useful tool for research applications.

The BTN3A1 polyclonal antibody has been tested in a range of applications, including ELISA, WB, and IHC, enabling protein detection, determination of presence and size, and localization of BTN3A1 protein.
產品名稱：

Rabbit anti-Homo sapiens (Human) BTN3A1 Polyclonal antibody
Uniprot No.：

O00481
基因名：

BTN3A1
別名：

BT3A1_HUMAN antibody; BTN3A1 antibody; Butyrophilin subfamily 3 member A1 antibody; CD277 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Butyrophilin subfamily 3 member A1 protein
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，BTN3A1 Antibody (CSB-PA002873LA01HU)，的標記方式是Non-conjugated。對于BTN3A1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA002873LB01HU	BTN3A1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA002873LC01HU	BTN3A1 Antibody, FITC conjugated
Biotin	CSB-PA002873LD01HU	BTN3A1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative:?0.02%?sodium?azide
Constituents:?PBS?containing?50%?glycerol pH 7.3
產品提供形式：

Liquid
應用范圍：

ELISA,WB?,IF

推薦稀釋比：

Application	Recommended Dilution
Recommended	?Recommended?dilution:?WB:1:500-1:1000?
IF	?1:20-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
基因功能參考文獻：
1. Microtubule-associated protein 4 (MAP4) controls the dynein-dependent transport of BTN3A1 in response to nucleic acid stimulation, thereby identifying MAP4 as an upstream regulator of BTN3A1. Thus, the depletion of either MAP4 or BTN3A1 impairs cytosolic DNA- or RNA-mediated type I IFN responses. PMID: 27911820
2. results show that ligand binding to BTN3A1 induces a conformational change within the intracellular domain that involves the JM region and is required for full activation. PMID: 28705810
3. findings show that changes in the juxtamembrane domain of BTN3A1, but not its transmembrane domain, induce a markedly enhanced or reduced gammadelta T cell reactivity PMID: 28461569
4. These findings support intracellular sensing of prenyl pyrophosphates by BTN3A1 rather than extracellular presentation. PMID: 26475929
5. Human gamma-delta T cells are activated by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. PMID: 25637025
6. evidence that gene(s) on Chr6 in addition to BTN3A1 are mandatory for PAg-mediated activation of Vgamma9Vdelta2 T cells. PMID: 24890657
7. Ligand binding to the BTN3A1 B30.2 domain affects residues in the juxtamembrane region, suggesting ligand-induced conformational change. PMID: 25065532
8. These studies demonstrate that internal sensing of changes in pAg metabolite concentrations by BTN3A1 molecules is a critical step in Vgamma9Vdelta2 T cell detection of infection and tumorigenesis. PMID: 24703779
9. BTN3A1 represents an antigen-presenting molecule required for the activation of Vgamma9Vdelta2 T cells. PMID: 23872678
10. investigation of role of CD277 in activation/inactivation of T-lymphocytes: Data indicate that modulation of CD277 interaction (with agonists or blocking antibodies) with T-cell antigen receptor can modulate activation/inactivation of T-lymphocytes. PMID: 22767497
11. BTN3A1 is necessary for Vgamma9Vdelta2 activation and begin to unravel the extracellular events that occur during stimulation through the Vgamma9Vdelta2 T cell receptor. PMID: 22846996
12. CD277 triggering is not involved in CD16- or NKp46-induced NK cell activation. PMID: 21918970
13. Results point to a role for CD277 up-regulated by microenvironmental signals in the acquisition of a regulatory phenotype by ovarian tumor-associated myeloid cells. PMID: 21113407
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, BTN/MOG family
組織特異性：

Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.
數據庫鏈接：

HGNC: 1138

OMIM: 613593

KEGG: hsa:11119

STRING: 9606.ENSP00000289361

UniGene: Hs.191510

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