ATP9A Antibody

中文名稱：

ATP9A兔多克隆抗體
貨號：

CSB-PA002422LA01HU
規格：

￥3900
圖片：
IHC image of CSB-PA002422LA01HU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Immunofluorescence staining of A549 cell with CSB-PA002422LA01HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SY5Y whole cell lysate
All lanes: ATP9A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ATP9A Polyclonal antibody
Uniprot No.：

O75110
基因名：

ATP9A
別名：

ATP 9A antibody; Atp9a antibody; ATP9A_HUMAN antibody; ATPase class II type 9A antibody; ATPase IIA antibody; ATPase type IV phospholipid transporting (P type) antibody; ATPase type IV phospholipid transporting (P type)(putative) antibody; ATPIIA antibody; KIAA0611 antibody; Phospholipid transporting ATPase IIA antibody; Probable phospholipid transporting ATPase IIA antibody; Probable phospholipid-transporting ATPase IIA antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Probable phospholipid-transporting ATPase IIA protein
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，ATP9A Antibody (CSB-PA002422LA01HU)，的標記方式是Non-conjugated。對于ATP9A Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA002422LB01HU	ATP9A Antibody, HRP conjugated	ELISA
FITC	CSB-PA002422LC01HU	ATP9A Antibody, FITC conjugated
Biotin	CSB-PA002422LD01HU	ATP9A Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:50-1:200
IF	1:20-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in regulating membrane trafficking of cargo proteins, namely endosome to plasma membrane recycling and endosome to trans-Golgi network retrograde transport. In complex with MON2 and DOP1B, regulates SNX3 retromer-mediated endosomal sorting of WLS, a transporter of Wnt morphogens in developing tissues. Participates in the formation of endosomal carriers that direct WLS trafficking back to Golgi, away from lysosomal degradation. Appears to be implicated in intercellular communication by negatively regulating the release of exosomes. The flippase activity towards membrane lipids and its role in membrane asymmetry remains to be proved.
基因功能參考文獻：
1. the predominant P4 ATPases in pure pancreatic beta cells and human and rat pancreatic islets were ATP8B1, ATP8B2, and ATP9A. ATP8B1 and CDC50A were highly concentrated in ISG PMID: 26240149
2. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
亞細胞定位：

Early endosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IV subfamily
數據庫鏈接：

HGNC: 13540

OMIM: 609126

KEGG: hsa:10079

STRING: 9606.ENSP00000342481

UniGene: Hs.649234

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