APOBEC3F Antibody

中文名稱：

APOBEC3F兔多克隆抗體
貨號：

CSB-PA816878LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Mouse heart tissue
All lanes: APOBEC3F antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 10, 12 kDa
Observed band size: 46 kDa

Immunofluorescent analysis of HepG2 cells using CSB-PA816878LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) APOBEC3F Polyclonal antibody
Uniprot No.：

Q8IUX4
基因名：

APOBEC3F
別名：

A3F antibody; ABC3F_HUMAN antibody; APOBEC3F antibody; Apolipoprotein B mRNA editing enzyme; catalytic polypeptide like 3F antibody; Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F antibody; ARP8 antibody; DNA dC->dU-editing enzyme APOBEC-3F antibody; Induced upon T cell activation antibody; KA6 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human DNA dC->dU-editing enzyme APOBEC-3F protein (139-287AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，APOBEC3F Antibody (CSB-PA816878LA01HU)，的標(biāo)記方式是Non-conjugated。對于APOBEC3F Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA816878LB01HU	APOBEC3F Antibody, HRP conjugated	ELISA
FITC	CSB-PA816878LC01HU	APOBEC3F Antibody, FITC conjugated
Biotin	CSB-PA816878LD01HU	APOBEC3F Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
基因功能參考文獻：
1. virus adaptation and computational studies to interrogate the APOBEC3F-Vif interface and build a robust structural model; taken together with mutagenesis results, propose a wobble model to explain how HIV-1 Vif has evolved to bind different APOBEC3 enzymes PMID: 26628363
2. Overexpression of APOBEC3F in tumor tissues is potentially predictive for poor recurrence-free survival from hepatitis b virus-hepatocellular carcinoma patients. PMID: 26760979
3. Our results provide genetic epidemiological evidence that A3F(APOBEC3F ) modulates HIV-1/AIDS disease progression PMID: 26942578
4. Six residues located within the conserved HIV-1 Vif F1-, F2-, and F3-box motifs are essential for both APOBEC3C and APOBEC3F degradation, and an additional four residues are uniquely required for APOBEC3F degradation. PMID: 26537685
5. This study showed for the first time a high level of APOBEC3F/3G editing in HIV-2 sequences from antiretroviral-naive patients. PMID: 25985400
6. The nucleocapsid domain of HIV-1 Gag and a linker sequence between the two cytidine deaminase domains are required for viral packaging of APOBEC3F. PMID: 25038404
7. Authors found that one pair of leucines in each of APOBEC3F's C-terminal and N-terminal cytidine deaminase domains jointly determined the degree of localization of APOBEC3F into HIV-1 virion cores. PMID: 25505075
8. This approach identified the alpha3 and alpha4 helices of human APOBEC3F as important determinants of the interaction with HIV-1 Vif. PMID: 25142588
9. Catalytic activity of APOBEC3F is required for efficient restriction of Vif-deficient human immunodeficiency virus 1. PMID: 24503066
10. Analysis of the A3F (W126A L306A) double mutant revealed that both residues are required for full anti-HIV function PMID: 22451677
11. the antiviral activity of endogenous A3F is negligible compared to that of A3G. PMID: 20702622
12. Long-term restriction by APOBEC3F selects human immunodeficiency virus type 1 variants with restored Vif function. PMID: 20686027
13. Alternative splicing of A3F mRNA generates truncated antiviral proteins that differ sharply in their sensitivity to Vif. PMID: 20624919
14. These results suggest that APOBEC3F neutralization is dispensable for HIV-1 replication in primary human T-lymphocytes. PMID: 20592068
15. The fact that several highly conserved tryphtophan residues in Vif are specifically required for the suppression of APOBEC3F (A3F) but not that of APOBEC3G (A3G) suggests a critical role for A3F in the restriction of HIV-1 in vivo. PMID: 16501124
16. APOBEC3B and APOBEC3F have roles in inhibiting L1 retrotransposition by a DNA deamination-independent mechanism PMID: 16648136
17. The Chinese population had a higher frequency of small alleles and showed a difference in allelic structure and frequency distribution in apolipoprotein B from European and American in this populations. PMID: 16767679
18. separation of function of the cytidine deaminase domains is maintained in hA3B and hA3F, but roles of the two domains are reversed in mouse A3 PMID: 17020885
19. Studies focused mainly on APOBEC3F imply that it occurs associated with mRNA-PABP1 in translationally active polysomes and to a lesser extent in mRNA processing bodies (P-bodies). PMID: 17977970
20. The APOBEC3F domain that interacts with the Vif DRMR region was located between amino acids 283 and 300 of A3F. PMID: 19036809
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亞細胞定位：

Cytoplasm. Cytoplasm, P-body.
蛋白家族：

Cytidine and deoxycytidylate deaminase family
組織特異性：

Widely expressed. Highly expressed in ovary.
數(shù)據(jù)庫鏈接：

HGNC: 17356

OMIM: 608993

KEGG: hsa:200316

STRING: 9606.ENSP00000309749

UniGene: Hs.659809

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