AP2B1 Antibody
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中文名稱：AP2B1兔多克隆抗體
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貨號：CSB-PA001871ESR1HU
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規格：￥440
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圖片：
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Western blot
All lanes: AP-2 complex subunit beta antibody at 8μg/ml + Mouse brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 105, 106, 99 kDa
Observed band size: 105 kDa -
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA001871ESR1HU at dilution of 1:100 -
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA001871ESR1HU at dilution of 1:100
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) AP2B1 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：Adapter related protein complex 2 beta 1 subunit antibody; Adapter-related protein complex 2 beta subunit antibody; Adaptin, beta 2 (beta) antibody; Adaptor protein complex AP-2 subunit beta antibody; Adaptor related protein complex 2, beta 1 subunit antibody; ADTB2 antibody; AP-2 complex subunit beta antibody; AP105B antibody; AP2 BETA antibody; Ap2b1 antibody; AP2B1_HUMAN antibody; Beta adaptin antibody; Beta-2-adaptin antibody; Beta-adaptin antibody; Beta2 adaptin antibody; CLAPB1 antibody; Clathrin assembly protein complex 2 beta large chain antibody; Clathrin associated/assembly/adaptor protein, large, beta 1 antibody; DKFZp781K0743 antibody; Plasma membrane adaptor HA2/AP2 adaptin beta subunit antibody
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宿主：Rabbit
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反應種屬：Human, Mouse
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免疫原：Recombinant Human AP-2 complex subunit beta protein (738-937AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:200-1:1000 IHC 1:20-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.
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基因功能參考文獻：
- this study determined a structure of AP2 that includes the clathrin-binding beta2 hinge. PMID: 25061211
- Results suggest that autosomal recessive hypercholesterolemia (ARH) and beta-arrestin depend on a privileged AP2beta2 appendage site for proper cargo recruitment to clathrin bud sites. PMID: 18843039
- multiple interactions between PIPKI gamma-p90 and AP-2 lead to spatiotemporally controlled PI(4,5)P(2) synthesis during clathrin-mediated synaptic vesicle endocytosis PMID: 19903820
- interactions of the EGF receptor with AP-2 mediated by the receptor 974YRAL and di-leucine motifs may contribute to beta2 tyrosine phosphorylation PMID: 12900408
- AP2 regulates thrombin receptor constitutive internalization and endothelial cell resensitization. PMID: 16581796
- Clathrin, which interacts with the AP2beta-appendage, achieves ligand displacement in vivo by self-polymerisation as the coated pit matures. PMID: 16903783
- EGFR, fibrillin-2, P-cadherin and AP2beta as biomarkers for rhabdomyosarcoma diagnostics. PMID: 19469909
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亞細胞定位：Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
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蛋白家族：Adaptor complexes large subunit family
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組織特異性：Expressed in the brain (at protein level).
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數據庫鏈接：
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