AARS Antibody

中文名稱：

AARS兔多克隆抗體
貨號：

CSB-PA001023LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA001023LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA001023LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA001023LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) AARS Polyclonal antibody
Uniprot No.：

P49588
基因名：

AARS
別名：

AARS antibody; AI316495 antibody; Alanine tRNA ligase 1, cytoplasmic antibody; Alanine tRNA ligase antibody; Alanine tRNA ligase cytoplasmic antibody; Alanine--tRNA ligase antibody; Alanyl tRNA synthetase antibody; Alanyl tRNA synthetase cytoplasmic antibody; Alanyl-tRNA synthetase antibody; AlaRS antibody; C76919 antibody; CMT2N antibody; cytoplasmic antibody; EC 6.1.1.7 antibody; MGC37368 antibody; Renal carcinoma antigen NY REN 42 antibody; Renal carcinoma antigen NY-REN-42 antibody; SYAC_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Alanine--tRNA ligase, cytoplasmic protein (164-321AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，AARS Antibody (CSB-PA001023LA01HU)，的標記方式是Non-conjugated。對于AARS Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA001023LB01HU	AARS Antibody, HRP conjugated	ELISA
FITC	CSB-PA001023LC01HU	AARS Antibody, FITC conjugated
Biotin	CSB-PA001023LD01HU	AARS Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
基因功能參考文獻：
1. A large sequence divergence of the C-terminal domain (C-Ala) reshaped C-Ala in a way that changed the global architecture of alanyl-tRNA synthetase (AlaRS). This reshaping removed the role of C-Ala in prokaryotes for docking tRNA and instead repurposed it to form a dimer interface presenting a DNA-binding groove. PMID: 27911835
2. Number of missense mutations in AARS expand the clinical spectrum and provide pheno-genotypic correlations in AARS-related neuropathies. PMID: 26032230
3. A novel mutation in alanyl-tRNA synthetase causes a mild myeloneuropathy, a novel phenotype for patients with mutations in one of the tRNA synthetase genes. PMID: 25904691
4. Loss-of-function alanyl-tRNA synthetase mutations cause an autosomal-recessive early-onset epileptic encephalopathy with persistent myelination defect. PMID: 25817015
5. the pathological consequences of diminished tRNA synthetase editing activity, and thus translational infidelity, are dependent on the cell type and the extent of editing disruption PMID: 25422440
6. in a family with distal hereditary motor neuropathy (dHMN), all 4 affected family members had a heterozygous missense mutation c.2677G>A (p.D893N) of (AARS), not found in the 4 unaffected members and control subjects; conclude AARS mutation caused dHMN in a Chinese family; AARS mutations result in not only a CMT phenotype but also a dHMN phenotype PMID: 22573628
7. Methylation-mediated deamination of a CpG dinucleotide gives rise to the recurrent p.Arg329His alanyl-tRNA synthetase mutation in patients with Charcot-Marie-Tooth disease type 2N (CMT2N). PMID: 22009580
8. We show here that mutations in AARS2 cause perinatal or infantile cardiomyopathy with near-total combined mitochondrial respiratory chain deficiency in the heart. PMID: 21549344
9. cytoplasmic Alanyl-tRNA synthetase may have a role in dominant axonal Charcot-Marie-Tooth disease, as shown by its mutation in a major determinant for binding and aminoacylation PMID: 20045102
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相關疾病：

Charcot-Marie-Tooth disease 2N (CMT2N); Epileptic encephalopathy, early infantile, 29 (EIEE29)
亞細胞定位：

Cytoplasm.
蛋白家族：

Class-II aminoacyl-tRNA synthetase family
數據庫鏈接：

HGNC: 20

OMIM: 601065

KEGG: hsa:16

STRING: 9606.ENSP00000261772

UniGene: Hs.315137

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