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中文名稱：lacZ鼠單克隆抗體
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貨號：CSB-MA009476A0m
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in Recombinant protein
All lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 130 kDa -
Western Blot
Positive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)
All lanes: lacZ antibody at 1:2000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 130 kDa -
Immunofluorescence staining of 293T cells transfected with CSB-MA009476A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). The image on the right is the 293T cells transfected without lacZ antibody -
Immunoprecipitating lacZ in 293T transfected whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA009476A0m in 293T transfected whole cell lysate
Lane 2: CSB-MA009476A0m (2μl) + 293T transfected whole cell lysate (500μg)
Lane 3: 293T transfected whole cell lysate (20μg)
For western blotting, the blot was detected with CSB-MA000021M0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
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Overlay histogram showing 293T transfected cells (red line) or untransfected cells (green line) stained with CSB-MA009476A0m at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CUSABIO貨號：CSB-MA009476A0m單克隆抗體是專為大腸桿菌lacZ基因編碼的β-半乳糖苷酶檢測開發(fā)的科研工具，該酶在乳糖代謝中負責水解乳糖為可代謝單糖，同時作為分子生物學中廣泛應用的報告基因標記。本產(chǎn)品采用雜交瘤技術(shù)制備，可特異性識別β-半乳糖苷酶抗原表位，適用于ELISA、Western Blot、免疫熒光、免疫沉淀及流式細胞術(shù)等多平臺檢測。在基因工程領(lǐng)域，該抗體可用于重組質(zhì)粒篩選驗證，通過特異性識別β-半乳糖苷酶表達產(chǎn)物輔助藍白斑篩選系統(tǒng)；在蛋白相互作用研究中，可配合免疫共沉淀技術(shù)解析酶復合體形成機制；其流式檢測功能為細菌代謝狀態(tài)分析提供新維度。該抗體還可應用于基因表達調(diào)控研究，通過Western Blot定量分析lacZ報告基因在不同條件下的表達水平，為啟動子活性檢測和基因治療載體構(gòu)建提供關(guān)鍵技術(shù)支持。
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產(chǎn)品名稱：Mouse anti-Escherichia coli lacZ Monoclonal antibody
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Uniprot No.：
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基因名：lacZ
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別名：lacZ antibody; b0344 antibody; JW0335Beta-galactosidase antibody; Beta-gal antibody; EC 3.2.1.23 antibody; Lactase antibody
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宿主：Mouse
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反應種屬：Escherichia coli
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免疫原：Recombinant Escherichia coli Beta-galactosidase protein (2-1024AA)
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免疫原種屬：Escherichia coli
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG1
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純化方式：>95%, Protein G purified
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克隆號：3B7D5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應用范圍：ELISA, WB, IF, IP, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:64000 IF 1:50-1:200 IP 1ul-4ul FC 1:100-1:300 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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