TBP Monoclonal Antibody

說明書

中文名稱：

TBP鼠單克隆抗體
貨號：

CSB-MA578709
規格：

￥1090
圖片：
Western blot analysis of 1) Hela, 2)Mouse Brain Tissue, 3) Rat Brain Tissue with TBP/TATA Binding Protein Mouse mAb diluted at 1:2,000.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

TBP單克隆抗體（CUSABIO貨號：CSB-MA578709）是針對TATA-box結合蛋白研發的高特異性科研工具，該靶點作為轉錄起始因子TFIID的核心亞基，在RNA聚合酶II介導的基礎轉錄過程中發揮關鍵作用，通過識別TATA盒調控基因表達。本產品采用小鼠宿主系統制備，IgG亞型呈現優異穩定性，液態制劑便于實驗操作，支持-20°C/-80°C長期保存。經嚴格驗證可特異性識別人源、小鼠及大鼠來源的TBP蛋白，適用于酶聯免疫吸附（ELISA）和蛋白質免疫印跡（Western Blot）兩大經典檢測平臺，為研究基因調控機制、轉錄因子網絡及疾病相關信號通路提供可靠工具。在基礎科研領域，該抗體可廣泛應用于癌癥生物學、神經退行性疾病模型、干細胞分化等研究場景，助力解析轉錄異常與病理進程的分子關聯。通過高純度重組蛋白免疫原制備工藝，確保抗體具有低交叉反應性和高批次一致性，滿足復雜實驗體系中對特異性識別的嚴格要求。
Uniprot No.：

P20226
基因名：

TBP
別名：

GTF2D antibody; GTF2D1 antibody; HDL4 antibody; MGC117320 antibody; MGC126054 antibody; MGC126055 antibody; SCA17 antibody; TATA binding factor antibody; TATA box factor antibody; TATA sequence binding protein antibody; TATA sequence-binding protein antibody; TATA-binding factor antibody; TATA-box binding protein N-terminal domain antibody; TATA-box factor antibody; TATA-box-binding protein antibody; TBP antibody; TBP_HUMAN antibody; TFIID antibody; Transcription initiation factor TFIID TBP subunit antibody
宿主：

Mouse
反應種屬：

Human,Mouse,Rat
免疫原：

Recombinant Protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

17B3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA,WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
基因功能參考文獻：
1. these studies support the idea that increases in TBP expression contribute to enhanced VEGFA transcription early in colorectal cancer development to drive tumorigenesis. PMID: 28415573
2. The genetic animal models of SCA17 clearly indicate that the phenotypes are dependent on the expression levels of mutant TBP and the length of CAG/glutamine repeat. PMID: 27859490
3. we observed that carriers of either ATXN7 or TBP alleles with relatively large CAG repeat sizes in both alleles had a substantially increased risk of lifetime depression. PMID: 28585930
4. Data indicate the crystal structure of a Brf2-TBP-Bdp1 complex bound to a DNA promoter. PMID: 28743884
5. Study could not determine a definitive cutoff value for the pathologic CAG repeat number of SCA17. PMID: 26267067
6. TBP attenuates Msx1-mediated glycoprotein hormone alpha transcriptional repression. PMID: 26505791
7. this analysis of the 2.2-kb doubly spliced RNA (2.2DS-RNA) -mediated suppression of viral RNA expression showed that 2.2DS-RNA inhibited transcription via binding to the TATA-binding protein and stress granule proteins. PMID: 26339052
8. Our results provide first evidence that Taspase1 processing affects TFIIA regulation of TFIID and suggest that Taspase1 processing of TFIIA is required to establish INR-selective core promoter activity in the presence of NC2. PMID: 25996597
9. TBP (and GATA2 and Sp1) have key roles in inflammation and ischemia-like conditions through MAOA regulation. PMID: 25810277
10. Deactivation of TBP contributes to SCA17 pathogenesis. PMID: 25104854
11. HOXA2 acts as a suppressor or TBP-antagonist to inhibit MMP-9 expression; while methylation-mediated inactivation of HOXA2 in NPC derepresses MMP-9 production and increases invasion of NPC cells. PMID: 24243817
12. The data reveal synergistic effects of H3K4me3, H3K14ac and a TATA box sequence on TFIID binding in vitro. Stoichiometry analyses of affinity purified human TFIID identified the presence of a stable dimeric core. PMID: 24039962
13. The UL4 protein directly interacted with the host TBP and the carboxy-terminal domain of RNA polymerase II in infected cells. PMID: 24418534
14. Findings indicate that using TATA-binding protein (TBP) alone or in combination with hydroxymethylbilane synthase (HMBS) as endogenous controls could be a reliable method for normalizing qRT-PCR data in hepatoma cell lines treated with TNF-alpha. PMID: 23811755
15. Ethanol increases Pol III transcription through a response element which is composed of the overlapping Elk1 and AP-1 binding sites of the TBP promoter. The binding sites may play a role in ethanol-induced deregulation of Pol III genes in liver tumors. PMID: 23454483
16. conclude that the oncoprotein HBXIP as a co-activator of TF II D transactivates Lin28B promoter via directly binding to TBP to upregulate the expression of Lin28B in promotion of proliferation of breast cancer cells PMID: 23494474
17. This study showed that five families with SCA17 represents a significant portion of ataxia cases in the population of the Czech Republic. PMID: 22872568
18. This study created a first transgenic rat model for SCA17 that carries a full human cDNA fragment of the TBP gene with 64 CAA/CAG repeats (TBPQ64). PMID: 23699518
19. effects of promoter TATA-box polymorphisms associated with human diseases on interactions between the TATA boxes and TATA-binding protein PMID: 23424617
20. These studies suggest a model in which the distinct conformations of TFIID may serve as targets through which regulatory factors recruit TFIID to specific types of core promoters. PMID: 23332750
21. TATA binding protein (TBP) interactions with oligonucleotides PMID: 23062343
22. CONCLUSIONS: The current case highlights the diagnostic challenge of neurodegenerative disorders and the need for a thorough clinical and paraclinical examination of patients. PMID: 22889412
23. The autosomal dominantly inherited progressive neurodegenerative disorder is caused by an expanded CAA/CAG repeat in the TATA-box binding protein PMID: 17853080
24. In Korean population, the mutation frequencies of SCA2 and SCA17 were similar. whereas SCA17 was a more significant cause of parkinsonism. PMID: 21334959
25. Expression of the transgenic TBP in Purkinje cells is sufficient to produce cell degeneration and an ataxia phenotype, and constitutes a good model for analysis of neurodegeneration in spinocerebellar ataxia type 17. PMID: 21554323
26. This study demonstrated mutant TATA box-binding protein at the endogenous level affects neuronal function, with important implications for the pathogenesis and treatment of polyglutamine diseases. PMID: 21705419
27. The data suggest that ICP4 interacts with components of TFIID and Mediator in the context of viral infection. PMID: 21450820
28. TBP mutations may be a very rare cause of Parkinson's disease in mainland China PMID: 20864379
29. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. PMID: 21106530
30. Brain weight correlated with the 'raw' (i.e. non-normalized) data for two mRNAs, beta2-microglobulin and TATA-binding protein, measured in cerebellum and hippocampus, respectively PMID: 20831744
31. Regulation of TBP by miR-146a may contribute to Huntington disease pathogenesis. PMID: 20451497
32. The DNA bend angle (theta) depends on the TATA sequence, with theta for coreTBP and human TBP being greater than that for yeast TBP. PMID: 19199812
33. In lymphoblastoid cells, HSPA5, HSPA8 and HSPB1 expression levels in cells with expanded SCA17 were significantly lower than that of the control cells. PMID: 20004653
34. TATA-binding protein-associated factor sharply decrease the rate at which Pol II, TFIIB, and TFIIF assemble on promoter-bound TFIID-TFIIA. PMID: 20083121
35. constitutive association of TBP with mitotic chromosomes PMID: 11809839
36. TBP can bind the TATA box through a regulated two-step mechanism, involving a transition from unbent complex to bent complex PMID: 11893333
37. Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene PMID: 11960008
38. Human papillomavirus-16 E7 protein inhibits the DNA interaction of the TATA binding transcription factor. PMID: 11968006
39. Data show that TATA-binding protein labelling was relatively more abundant than huntingtin labelling in the brains of Huntington disease patients, and increased with the grade of the disease. PMID: 12531510
40. Data show that human TATA box binding protein (TBP) can use a shared surface to interact with two different transcription factor IIB (TFIIB) family members to initiate transcription by different RNA polymerases. PMID: 12535529
41. spatial positioning of the DNA-bound activation domain is important for efficient activation, possibly by maximizing its interactions with the transcriptional machinery including the TBP-TFIIA-TFIIB-promoter quaternary complex PMID: 12538582
42. TBP expression is elevated in human colon carcinomas relative to normal colon epithelium. Both Ras-dependent and Ras-independent mechanisms mediate increases in TBP expression in colon carcinoma cell lines. PMID: 12697807
43. Sequence-dependent solution structure and motions of TBP complexes were studied. PMID: 12767124
44. simultaneously binds and bends promoter DNA without a slow isomerization step or TFIIB requirement PMID: 12791683
45. Our data provides biochemical evidence that Mediator functions by facilitating activator-mediated recruitment of pol II and also promoter recognition by TBP, both of which can occur in the absence of TBP-associated factors in TFIID PMID: 12917344
46. p300 plays a role in formation of the TBP-TFIIA-containing basal transcription complex, TAC. PMID: 12941701
47. Results present the X-ray structures of human and yeast TATA box-binding protein /transcription factor IIA/DNA complexes at 2.1A and 1.9A resolution, respectively. PMID: 12972251
48. in addition to its role in regulating TBP binding to a TATA box, the TBP surface is unexpectedly involved in TBP association with all three TFIIB family members PMID: 14585974
49. abnormal expansions of an allele in SCA8 and SCA17 genes were detected in patients with both Parkinson's disease and spinocerebellar ataxia PMID: 14756671
50. Analysis of Spinocerebellar ataxia type 17 (SCA17) locus in a group of ataxic patients excluded on other known SCAs. PMID: 14763955
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相關疾病：

Spinocerebellar ataxia 17 (SCA17)
亞細胞定位：

Nucleus.
蛋白家族：

TBP family
組織特異性：

Widely expressed, with levels highest in the testis and ovary.
數據庫鏈接：

HGNC: 11588

OMIM: 600075

KEGG: hsa:6908

STRING: 9606.ENSP00000230354

UniGene: Hs.590872

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