SMURF1 Monoclonal Antibody

說(shuō)明書

中文名稱：

SMURF1鼠單克隆抗體
貨號(hào)：

CSB-MA875720A0m
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate
All lanes: SMURF1 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 KDa
Exposure time：5min

IHC image of CSB-MA875720A0m diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA875720A0m diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA875720A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunofluorescence staining of HepG2 cells with CSB-MA875720A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Overlay Peak curve showing Hela cells stained with CSB-MA875720A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CUSABIO貨號(hào)：CSB-MA875720A0m SMURF1單克隆抗體是專為科研工作者設(shè)計(jì)的特異性檢測(cè)工具，靶向調(diào)控細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)的關(guān)鍵因子SMURF1蛋白。SMURF1作為HECT結(jié)構(gòu)域泛素連接酶家族成員，通過(guò)介導(dǎo)TGF-β/BMP信號(hào)通路參與細(xì)胞遷移、凋亡調(diào)控及腫瘤微環(huán)境形成等生物學(xué)過(guò)程，在腫瘤發(fā)生發(fā)展與轉(zhuǎn)移機(jī)制研究中具有重要科研價(jià)值。本產(chǎn)品采用雜交瘤技術(shù)制備，經(jīng)嚴(yán)格驗(yàn)證適用于酶聯(lián)免疫吸附（ELISA）、蛋白質(zhì)印跡（WB）、免疫組織化學(xué)（IHC）、免疫熒光（IF）及流式細(xì)胞術(shù)（FC）等多種實(shí)驗(yàn)體系，能夠特異性識(shí)別人類來(lái)源的SMURF1抗原表位。其高批次穩(wěn)定性與低交叉反應(yīng)特性確保了實(shí)驗(yàn)數(shù)據(jù)的可靠性，特別適用于腫瘤生物學(xué)研究、信號(hào)通路解析、泛素化修飾機(jī)制探索等基礎(chǔ)科研領(lǐng)域。該抗體在Hela細(xì)胞裂解液及人肝組織樣本中展現(xiàn)出優(yōu)異檢測(cè)性能，為研究者深入探究SMURF1在細(xì)胞周期調(diào)控、上皮間質(zhì)轉(zhuǎn)化（EMT）及腫瘤轉(zhuǎn)移中的分子機(jī)制提供了高質(zhì)量試劑支持，是信號(hào)轉(zhuǎn)導(dǎo)研究與腫瘤微環(huán)境分析的重要工具。
產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) SMURF1 Monoclonal antibody
Uniprot No.：

Q9HCE7
基因名：

SMURF1
別名：

E3 ubiquitin-protein ligase SMURF1 antibody; hSMURF1 antibody; KIAA1625 antibody; Smad specific E3 ubiquitin ligase 1 antibody; SMAD specific E3 ubiquitin protein ligase 1 antibody; Smad ubiquitination regulatory factor 1 antibody; SMAD-specific E3 ubiquitin-protein ligase 1 antibody; SMUF1_HUMAN antibody; SMURF 1 antibody; smurf1 antibody
宿主：

Mouse
反應(yīng)種屬：

Human
免疫原：

Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198-374AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2a
純化方式：

>95%, Protein G purified
克隆號(hào)：

1A9H4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	WB：1:1000-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
基因功能參考文獻(xiàn)：
1. Nedd8 binding to Smurf plays important roles in the regulation of cell migration and the BMP and TGFbeta signaling pathways. PMID: 28169289
2. Expression of Smurf1 was increased with WHO grade and was consistent with poor prognosis of gliomas. PMID: 28321604
3. Smurf1 interacts with and targets Securin, an inhibitor of sister-chromatid separation, for poly-ubiquitination and proteasomal degradation. PMID: 28658604
4. Smurf1 overexpression decreases USP25 protein turnover, and the E3 ligase enzymatic activity of Smurf1 is required for USP25 degradation. PMID: 29518389
5. SMURF1 can be potentially used as a clinical biomarker and target for novel treatment of human GC. PMID: 28731194
6. Uev1A appears to be involved in the BMP signaling pathway in which it collaborates with a ubiquitin E3 ligase Smurf1 to promote Smad1 degradation in a Ubc13-independent manner. PMID: 28771228
7. High smurf1 expression is associated with neoplasms. PMID: 27036023
8. activation of AMPK upregulated Smad6 and Smurf1 and thereby enhanced their interactions, resulting in its proteosome-dependent degradation of ALK2. PMID: 28847510
9. we propose that the PKA-Smurf1-PIPKIgamma pathway has an important role in pulmonary tumorigenesis and imposes substantial clinical impact on development of novel diagnostic markers and therapeutic targets for lung cancer treatment. PMID: 28581524
10. SMURF1 is increased in patients with pulmonary arterial hypertension PMID: 27214554
11. Data suggest that SMURF1 is required for S phase progression; SMURF1 promotes ubiquitination-dependent degradation of WEE1; these functions of SMURF1 appear to be linked and may be important in cell proliferation and tumorigenesis. (SMURF1 = SMAD specific E3 ubiquitin protein ligase 1; WEE1 = wee 1 homolog [S pombe] protein) PMID: 28294307
12. Taken together, our study for the first time clarified that the E3 ligase Smurf1 regulates USP5 protein stability and USP5-mediated TNF-alpha production through the ubiquitin proteasome pathway. PMID: 27133717
13. results reveal the regulatory circuit between RUNX2 and SMURF1 controls RUNX2 expression and regulates odntoblastic differentiation in hDPSCs. PMID: 25260729
14. EGF/Smurf1 inhibits Wnt/beta-catenin-induced osteogenic differentiation and that Smurf1 downregulates Wnt/b-catenin signaling by enhancing proteasomal degradation of beta-catenin. PMID: 26015066
15. a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1. PMID: 26004137
16. SND1 promoted expression of the E3 ubiquitin ligase Smurf1, leading to RhoA ubiquitination and degradation. PMID: 25596283
17. NF-kappaB bind to the -411 to -420 region of the SMURF1 promoter and plays an essential role in the expression of SMURF1. PMID: 26252093
18. Smurf1 determines cell apoptosis rates downstream of DNA damage-induced ATR/Chk1 signalling by promoting degradation of RhoB. PMID: 25249323
19. The role of SMURF1 and inhibition of BMP signaling in maintaining a CSC-like population was confirmed by the loss of a CD44(high) expressing. PMID: 25471937
20. Results suggest that elevated transcription and expression levels of ubiquitin ligase E3s WWP1, Smurf1 and Smurf2 genes may be the mechanisms of occurrence, development and metastasis of prostate cancer. PMID: 25051198
21. Fbxo3 promotes the proteasomal degradation of Smurf1. Fbxo3 promotes the poly-ubiquitination of Smurf1. PMID: 25721664
22. TRIB2 associated-ubiquitin E3 ligases beta-transducin repeat-containing E3 ubiquitin protein ligase (beta-TrCP), COP1 and Smad ubiquitination regulatory factor 1 (Smurf1) reduced TCF4/beta-Catenin expression, and these effects could be enhanced by TRIB2. PMID: 25311538
23. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation PMID: 24700460
24. Smurf1 is localized within the Hirano bodies of Alzheimer's disease brains. PMID: 24238996
25. CKIP-1 controlled Smurf1 expression in colon cancer PMID: 23995790
26. Inhibiting Smurf1 mediated ubiquitination of Smad1/5. PMID: 24828823
27. Results suggest that EGF-induced SMURF1 plays a role in breast cancer cell migration and invasion through the downregulation of RhoA. PMID: 24241683
28. impaired phosphorylation and ubiquitination by p70S6K and Smurf1 increase the protein stability of TRIB2 in liver cancer PMID: 24089522
29. Data indicate that Smad ubiquitin regulatory factor Smurf1 regulates cell migration through ubiquitination of tumor necrosis factor receptor-associated factor 4 (TRAF4). PMID: 23760265
30. Resistance exercise resulted in a significant downregulation of MSTN and FBXO32 mRNA expression and a significant upregulation in FSTL3 and SMURF1 mRNA expression, and carbohydrate and protein feeding have little influence on the these markers expression. PMID: 22476926
31. Smurf1 turnover is mediated by Ckip-1 and Rpt6. PMID: 23032291
32. The expression of SMURF1 is enhanced in hepatocellular carcinoma, which may have played a role in the disease through affecting apoptosis and proliferation of hepatic cancer cells. PMID: 23042388
33. USP9X is an important regulatory protein of SMURF1. PMID: 23184937
34. Ndfip1 negatively regulates RIG-I-dependent immune signaling by enhancing E3 ligase Smurf1-mediated MAVS degradation. PMID: 23087404
35. A Cdh1-APC/Smurf1/RhoA pathway that mediates axonal growth suppression in the developing mammalian brain. PMID: 22949615
36. Smurf1 is a negative feedback regulator for IFN-gamma signaling by targeting STAT1 for ubiquitination and proteasomal degradation. PMID: 22474288
37. Inflammatory cytokines led to direct activation of Smurf1 and downregulation of miR-17, thereby increasing degradation of Smurf1-mediated osteoblast-specific factors. PMID: 21898695
38. studies uncover a cell-cycle-independent function of Cdh1, establishing Cdh1 as an upstream component that governs Smurf1 activity PMID: 22152476
39. SMURF1 is an amplified oncogene driving multiple tumorigenic phenotypes in pancreatic cancer PMID: 21887346
40. Data report that the C2 domain of Smurf1 is necessary and sufficient for binding RhoA, and therefore is crucial for targeting RhoA for ubiquitination. PMID: 21708152
41. ER stress induces Smurf1 degradation and WFS1 up-regulation PMID: 21454619
42. the Smurf1 C2 domain has a role in substrate selection and cellular localization PMID: 21402695
43. This study provided the first evidence that Smurf1 functions as an E3 ligase to promote the ubiquitination and proteasomal degradation of KLF2. PMID: 21382345
44. These results suggest a new role of Smurf1 in inflammation and immunity through controlling the degradation of TRAFs. PMID: 19937093
45. plasma membrane localization of Smad7 by Smurf1 requires the C2 domain of Smurf1 and is essential for the inhibitory effect of Smad7 in the transforming growth factor-beta signaling pathway PMID: 12151385
46. CRM1-dependent nuclear export of Smurf1 is essential for the negative regulation of TGF-beta signaling by Smad7. PMID: 12519765
47. Smurf1 links the Cdc42/Rac1-PAR6 polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements PMID: 14657501
48. Smad7 and Smurf1 have roles in regulation of TGF-beta signaling in scleroderma fibroblasts PMID: 14722617
49. rhoA GTP-Binding Protein is targeted for ubiquitination and degradation via Smurf1 PMID: 15710384
50. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation PMID: 16299379
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亞細(xì)胞定位：

Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
組織特異性：

Expressed in melanocytes.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 16807

OMIM: 605568

KEGG: hsa:57154

STRING: 9606.ENSP00000354621

UniGene: Hs.189329

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