MFGE8 Monoclonal Antibody

說明書

中文名稱：

MFGE8鼠單克隆抗體
貨號：

CSB-MA079741A0m
規(guī)格：

￥1320
圖片：
Immunohistochemical of paraffin-embedded human Colon cancer tissues using CSB-MA079741A0m at dilution of 1:200

Immunohistochemical of paraffin-embedded human breast cancer tissue using CSB-MA079741A0m at dilution of 1:200
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

MFGE8單克隆抗體（CUSABIO貨號：CSB-MA079741A0m）是針對乳脂肪球EGF因子8蛋白研發(fā)的科研專用試劑，該靶標蛋白在細胞粘附、信號傳導(dǎo)及免疫調(diào)節(jié)中發(fā)揮重要作用，尤其參與脂質(zhì)代謝相關(guān)通路和細胞間相互作用機制研究。本產(chǎn)品采用高特異性單克隆抗體技術(shù)，經(jīng)嚴格驗證可識別人源MFGE8抗原表位，適用于酶聯(lián)免疫吸附測定（ELISA）進行蛋白質(zhì)定量分析，以及免疫組化（IHC）實驗用于組織定位研究。其核心優(yōu)勢包括精準的抗原結(jié)合能力與穩(wěn)定的批次一致性，能夠滿足細胞生物學、腫瘤微環(huán)境、代謝疾病等領(lǐng)域的科研需求，特別適用于乳腺組織、免疫細胞及相關(guān)病理模型的分子機制探索。該抗體為凍干粉劑型，建議用戶根據(jù)實驗體系優(yōu)化使用方案，通過靶向MFGE8蛋白的功能解析，助力細胞信號網(wǎng)絡(luò)、組織修復(fù)機制等前沿課題研究。
產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) MFGE8 monoclonal antibody
Uniprot No.：

Q08431
基因名：

MFGE8
別名：

BA46 antibody; Breast epithelial antigen BA46 antibody; EDIL1 antibody; HMFG antibody; hP47 antibody; HsT19888 antibody; Lactadherin antibody; Medin antibody; MFG-E8 antibody; MFGE8 antibody; MFGM antibody; MFGM_HUMAN antibody; Milk fat globule EGF factor 8 antibody; Milk fat globule EGF factor 8 protein antibody; Milk fat globule-EGF factor 8 antibody; O acetyl disialoganglioside synthase antibody; OAcGD3S antibody; SED1 antibody; SPAG10 antibody; Sperm associated antigen 10 antibody; Sperm surface protein hP47 antibody
宿主：

Mouse
反應(yīng)種屬：

Human
免疫原：

Recombinant Human MFGE8 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

monoclonal
抗體亞型：

IgG2b
純化方式：

>95%,Protein G purified
克隆號：

2B2D10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

liquid
應(yīng)用范圍：

ELISA,IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:500

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.; Main constituent of aortic medial amyloid.
基因功能參考文獻：
1. Esophageal cancer patients with high MFG-E8 expression had worse overall survival and relapse-free survival in the subgroup with preoperative chemotherapy. The abundant MFG-E8 expression in esophageal squamous cell carcinoma might have a negative influence on the long-term survival of patients after chemotherapy by affecting T-cell regulation in the tumor microenvironment. PMID: 30156356
2. MFGE8 polymorphisms were not associated with MetS but were related to DBP, HDL-C level, and WC in Chinese adults. PMID: 30053459
3. This study showed for the first time that MFG-E8 expression is impaired in the endometrium of patients with endometriosis and infertility during the window of implantation. PMID: 28967712
4. Our findings suggest that both MFGE8 and TGFbeta1 are agerelated inflammatory factors and are related to the degree of Atherosclerosis (AS). In conclusion, both MFGE8 and TGFbeta1 may serve as potential markers of the severity of AS PMID: 29152651
5. Elevated serum MFG-E8 levels may be associated with cerebrovascular diseases or neuropsychiatric systemic lupus erythematosus. PMID: 28266034
6. Circulating MFG-E8 levels are dramatically elevated in pregnancy, and are significantly higher in gestational diabetes mellitus than in normal pregnancy. PMID: 28035763
7. MFG-E8 could be a potential novel prognostic marker for colorectal cancer (CRC) and overexpression of MFG-E8 might be involved in lymph node metastasis and angiogenesis of CRC. PMID: 28923329
8. MFG-E8 levels are elevated in T2D but suppressed by increased adipose tissues, thereby allowing inflammatory factors to rise to high levels. MFG-E8 may serve as a potential biomarker for obesity and T2D in the clinical setting PMID: 28067023
9. These findings provide a better understanding of the molecular mechanism underlying colorectal cancer progression and suggest a predictive role for MFG-E8 in colorectal cancer metastasis and prognosis PMID: 28653875
10. Levels of MFGE8 were reduced in cirrhotic liver tissue from patients compared with controls. PMID: 27956229
11. an efficient purification method for production of non-aggregated, full-length MFG-E8. PMID: 27102803
12. Reduced serum milk fat globule-epidermal growth factor 8 (MFG-E8) concentrations are associated with an increased risk of microvascular complications in patients with type 2 diabetes. PMID: 28089751
13. MFG-E8 is a protective factor in the pathogenesis of rheumatoid arthritis and subsequent bone loss. PMID: 26391522
14. After myocardial infarction, Mfge8 (and Mertk)-expressing macrophages synergistically engage the clearance of injured cardiomyocytes. PMID: 26819373
15. Studied the therapeutic effect of rhMFG-E8 in mouse models of IBD. Treatment with rhMFG-E8 significantly attenuated colitis in both models in a dose-dependent way. PMID: 25751740
16. MFG-E8 promotes cutaneous wound healing by enhancing angiogenesis. PMID: 24838098
17. Lactadherin may decrease inflammation by inhibiting secretory phospholipase A2 activity on pre-apoptotic leukemia cells. PMID: 24194865
18. promotes tumor progression in oral squamous cell carcinoma PMID: 25264705
19. MFG-E8 had a negative association with hs-CRP and a positive association with LDL-c. The serum level of MFG-E8 was negatively associated with the severity of coronary artery stenosis and the risk of clinical events. PMID: 24561551
20. medin adopts a predominantly beta-sheet conformation with some unstructured elements. PMID: 24602872
21. MFG-E8 could be used as a biomarker for diagnosis and monitoring of disease activity in certain systemic lupus erythematosus patients PMID: 24554711
22. Exogenously added MFG-E8 inhibits receptor activator NF-kappaB ligand-induced osteoclastogenesis of human osteoclast precursors. PMID: 24958900
23. Our results strongly suggested that MFG-E8 is a promising biomarker for the diagnosis, prognosis, and therapy target of opisthorchiasis-associated cholangiocarcinoma PMID: 24122204
24. Blockage of MFG-E8 in endometrial tumor cells diminishes trophoblaast cell attachment. PMID: 24424369
25. TNF-alpha up-regulates endometrial epithelial cell migration and MFG-E8 production. PMID: 24262600
26. MFG-E8-dependent promotion of apoptotic cell clearance is a novel anti-inflammatory facet of glucocorticoid treatment PMID: 23832117
27. our data argue that MFGE8 is not likely involved in the phagocytic clearance of neuronal debris associated with nigrostriatal pathway injury. PMID: 23194669
28. NMR solution structure of C2 domain of MFG-E8 and insights into its molecular recognition with phosphatidylserine PMID: 23262193
29. Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. PMID: 22921913
30. MFG-E8 expression in the endometrial epithelium as well as in chorionic villi suggests its possible role in endometrial reorganization during the receptive phase and in events related to normal pregnancy in mammals PMID: 22770563
31. Decreased colonic MFGE8 expression in patients with ulcerative colitis may be associated with mucosal inflammatory activity and clinical disease activity through basal cell apoptosis and preventing tissue healing in the pathogenesis of ulcerative colitis PMID: 22204000
32. a key role of MFG-E8 release from apoptotic endothelial cells in macrophage reprogramming PMID: 22558449
33. The distribution of the SNP (rs4945 and rs1878326) of MFGE8 was analyzed in two groups of patients with "wet" age-related macular Degeneration and their age-matched controls. PMID: 22438901
34. serum lactadherin is correlated with poor blood glucose control and diabetic vascular complications. PMID: 22018779
35. We conclude that MFG-E8-dependent signaling stimulates cell proliferation and the acquisition of mesenchymal properties and contributes to mammary carcinoma development. PMID: 21841820
36. in vitro studies showed that medin amyloid-like fibrils promote the aggregation of protein amyloid A into fibrils PMID: 22070546
37. Prolactin has a modulatory role for as a stromal/epithelial paracrine factor controlling MFG-E8. This is the first report on MFG-E8 protein localization to the human endometrial epithelium and its up-regulation during the window of implantation. PMID: 21177637
38. MFG-E8 is expressed in triple-negative breast cancers as a target gene of the p63 pathway, but may serve a suppressive function in ER(+) and erbB2(+) breast cancers. PMID: 21127199
39. overexpression of MFGE8 during bladder tumor development, correlated with expression of genes involved in cell adhesion or migration and in immune responses. PMID: 20956946
40. intronic mutation in the human MFG-E8 gene can lead to the development of SLE. PMID: 20213738
41. SED1 is expressed on the surface of acrosome-intact human sperm and in the anterior caput of the human epididymis, similar to that seen in mouse PMID: 18990388
42. Truncated fragment of medin, the hexapeptide, NFGSVQ can form typical amyloid fibrils. PMID: 15478463
43. Might prove useful in the treatment of prolonged ischemia. PMID: 16115445
44. Lactadherin) is expressed in normal and atherosclerotic human arteries. PMID: 17420351
45. The trans-activator (TA) isoforms of p63 activate MFGE8 transcription though a p53/p63 motif at -370, which was confirmed by a chromatin immunoprecipitation experiment. PMID: 17637751
46. Identification of the last 18-19 amino acid residues as constituting the amyloid-promoting region of medin. PMID: 17679143
47. aggregated medin induced death of aortic smooth muscle cells in vitro. Cells incubated together with medin increased the production of matrix metalloproteinase-2, a protease that degrades elastin and collagen and subsequently weakens the vessel wall. PMID: 17906662
48. analysis of membrane-interactive loops of Lact-C2 PMID: 18160406
49. Some childhood-onset and adult SLE patients carried a significant level of MFG-E8 in their blood samples. PMID: 18303131
50. Lactadherin promoted phagocytosis of phosphatidylserine-expressing RBCs by macrophages in a concentration-dependent manner. PMID: 18647368
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亞細胞定位：

Membrane; Peripheral membrane protein. Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Peripheral membrane protein.
組織特異性：

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
數(shù)據(jù)庫鏈接：

HGNC: 7036

OMIM: 602281

KEGG: hsa:4240

STRING: 9606.ENSP00000268150

UniGene: Hs.3745

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