GSTM3 Monoclonal Antibody

說明書

中文名稱：

GSTM3鼠單克隆抗體
貨號：

CSB-MA009982A1m
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, Rat brain tissue
All lanes: GSTM3 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 27 KDa
Observed band size: 27 KDa
Exposure time：10min

IHC image of CSB-MA009982A1m diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Overlay Peak curve showing Hela cells stained with CSB-MA009982A1m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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產品詳情

產品描述：

CUSABIO貨號：CSB-MA009982A1m GSTM3單克隆抗體是針對谷胱甘肽S-轉移酶Mu3（GSTM3）研發的高特異性科研試劑，該靶標蛋白屬于Ⅱ期代謝酶家族成員，在細胞解毒代謝和氧化應激調控中發揮重要作用，其表達異常與多種腫瘤發生發展密切相關。本產品采用雜交瘤技術制備，經ELISA、蛋白質印跡（WB）、免疫組織化學（IHC）及流式細胞術（FC）多平臺驗證，可特異性識別人源GSTM3蛋白，具有批次穩定性高、交叉反應性低的特點。在1:1000稀釋比下WB實驗顯示清晰條帶，IHC染色能準確呈現組織樣本中GSTM3的亞細胞定位。適用于腫瘤發生機制研究、藥物代謝通路分析、氧化應激相關信號轉導實驗等前沿領域，為探索GSTM3在癌癥生物標志物篩選、化療耐藥性研究及抗氧化治療開發提供可靠工具。該GSTM3抗體具有多平臺兼容性，可滿足蛋白質表達譜分析、病理切片檢測和細胞表面標記物研究等多元化實驗需求，是開展分子毒理學和腫瘤微環境研究的優選試劑。
產品名稱：

Mouse anti-Homo sapiens (Human) GSTM3 Monoclonal Antibody antibody
Uniprot No.：

P21266
基因名：

GSTM3
別名：

brain GST antibody; brain type mu glutathione S transferase antibody; glutathione S alkyltransferase M3 antibody; glutathione S aryltransferase M3 antibody; glutathione S transferase M3 (brain) antibody; glutathione S transferase M3 antibody; Glutathione S transferase Mu 3 antibody; glutathione S transferase, Mu 3 antibody; Glutathione S-transferase Mu 3 antibody; GST class mu 3 antibody; GST class-mu 3 antibody; GST5 antibody; GSTB antibody; Gstm3 antibody; GSTM3-3 antibody; GSTM3_HUMAN antibody; GTM3 antibody; hGSTM3-3 antibody; MGC3310 antibody; MGC3704 antibody; S (hydroxyalkyl)glutathione lyase M3 antibody
宿主：

Mouse
反應種屬：

Human
免疫原：

Recombinant Human Glutathione S-transferase Mu 3 protein (1-225AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal Antibody
抗體亞型：

IgG1
純化方式：

>95%, Protein G purified
克隆號：

2H9D7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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靶點詳情

功能：

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.
基因功能參考文獻：
1. Differential activity of antioxidant enzymes caused by the polymorphism in GSTM3 may contribute to resistance to hormonal therapy through oxidative stress. The GSTM3 rs7483 polymorphism may be a promising biomarker for prostate cancer patients treated with androgen-deprivation therapy (ADT) PMID: 27993795
2. Expression of GSTM3 might be regulated by epigenetic changes in lens tissue. Hypermethylation in GSTM3 promoter and altered histone modification might have a role in the ARC formation. PMID: 27607418
3. No associations between the GSTT1, GSTP1, and GSTM3 genotypes and neoplasia risk were observed. In conclusion, we determined the genotype distribution of GST polymorphisms in control subjects and breast cancer patients from northeastern Mexico. PMID: 26125851
4. NSD1 interacted with RNAPII and bound to GSTM3 -63A/C TATA box. PMID: 25193115
5. All three markers correlated significantly with regional lymph node metastasis: FXYD3 (p = 0.0110), S100A11 (p = 0.0071), and GSTM3 (p = 0.0173) in colon cancer lymphatic metastasis. PMID: 22430872
6. This meta-analysis suggests that the GSTM3 A/B polymorphism may be an important protective factor for head and neck cancer, especially of laryngeal cancer and Caucasian populations. PMID: 24416175
7. rs1332018 genetic variants in the GSTM3 promoter predispose the host to downregulating GSTM3 expression in kidney, facilitate carcinogenesis, and predict an unfavourable postoperative prognosis of renal cell carcinoma. PMID: 24157827
8. In conclusion, this epistatic interaction showed a high degree of consistency when stratifying by sex, the epsilon4 allele of apolipoprotein E genotype, and geographic region. PMID: 23036584
9. The GSTM3 A/B gene polymorphism is not associated with lung cancer susceptibility. PMID: 23167362
10. Methylation of GSTM3 promoter may contribute to oxidative stress-associated liver damage and correlate with the disease severity in Acute-on-chronic hepatitis B liver failure. PMID: 22976281
11. There were no significant differences in distribution of 3-bp deletion polymorphism in intron 6 variant allele in Glutathione-S-transferase M3 between chronic obstructive pulmonary disease patients and controls in a north Indian population. PMID: 21513434
12. In individuals from Angola, Mozambique and the Sao Tome e Principe islands, the GSTM3*B allele was three times more frequent (0.74-0.78) than the GSTM3*A allele (0.22-0.26), with no significant differences in allele frequency across the three groups. PMID: 20549140
13. Diminished GSTM3 mRNA levels correlated with decreased minichromosome maintenance deficient 3 (MCM3) mRNA levels in a diagnostic and SNP-dependent fashion in Alzheimer disease. PMID: 18423940
14. The presence of the GSTM3*B allele appears to associated with a reduced risk of laryngeal squamous cell carcinoma. PMID: 19922706
15. No increased prostate scancer risk for men carrying any of the GSTM1 or GSTT1 genotypes. PMID: 14968442
16. Glutathione S-transferase hGSTM3 has a role in ageing-associated neurodegeneration PMID: 15621212
17. GSTM3 -63C allele strongly affects gene expression and suggests that individuals who carry the low expression allele may be deficient in glutathione transferase catalyzed biological functions. PMID: 15665284
18. Single marker association analyses revealed that the AGG/AGG genotype of the GSTM3 rs1799735 (del/AGG) polymorphism was associated with an increased risk of AD (p=0.05), especially in the group of APOE4-allele non-carriers (p=0.004; OR=2.07). PMID: 17904251
19. distribution of -63A/C polymorphism of human glutathione S-transferase M3(GSTM3) gene in Chinese Han population and the association of -63A/C polymorphism with essential hypertension PMID: 17922434
20. The GSTM3*A/*A genotype was 76% in cancer cases versus 74% in controls; a significant association between smoking status and GSTM3 genotype was not detected. PMID: 18569590
21. Our results suggested GSTM3 (AB + BB) genotype to be significantly associated with prostate cancer risk. PMID: 18668224
22. GST-M3 activity is possibly involved in protection against mucosal atrophy caused by H. pylori as the levels of IgG titer and pepsinogen I are linked to mucosal status. PMID: 19696791
23. The researchers found evidence of an increased risk for non-Hodgkin's lymphoma in women who used hair dye before 1980 and who were carriers of the GSTM3 intron 6 deletion. PMID: 19822571
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亞細胞定位：

Cytoplasm.
蛋白家族：

GST superfamily, Mu family
組織特異性：

Testis and brain.
數據庫鏈接：

HGNC: 4635

OMIM: 138390

KEGG: hsa:2947

STRING: 9606.ENSP00000256594

UniGene: Hs.2006

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