CR2 Monoclonal Antibody

說明書

中文名稱：

CR2鼠單克隆抗體
貨號：

CSB-MA916751
規(guī)格：

￥1320
圖片：
IHC image of CSB-MA916751 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CR2 Monoclonal Antibody（CUSABIO貨號：CSB-MA916751）是針對人類補體受體2（CR2）研發(fā)的高特異性科研抗體，適用于ELISA和免疫組化（IHC）實驗。CR2作為B細胞表面重要受體，在補體系統(tǒng)介導的免疫應答中具有關鍵作用，其與C3d片段的結(jié)合可激活B細胞信號通路，在免疫調(diào)節(jié)、病原體識別及自身免疫性疾病機制研究中備受關注。本產(chǎn)品采用雜交瘤技術(shù)制備，具備嚴格的物種特異性，僅對人類樣本產(chǎn)生可靠反應，適用于免疫學研究中的蛋白定位、信號通路分析以及疾病模型構(gòu)建，例如B細胞活化機制探索、系統(tǒng)性紅斑狼瘡等自身免疫性疾病相關研究。產(chǎn)品經(jīng)過嚴格的質(zhì)控驗證，在抗原結(jié)合能力和實驗穩(wěn)定性方面表現(xiàn)優(yōu)異，可為免疫微環(huán)境分析、受體功能研究提供精準工具。
產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) CR2 Monoclonal antibody
Uniprot No.：

P20023
基因名：

CR2
別名：

C3DR antibody; CD 21 antibody; CD21 antibody; Complement C3d receptor 2 antibody; Complement C3d receptor antibody; Complement component (3d/Epstein Barr virus) receptor 2 antibody; Complement receptor type 2 antibody; CR antibody; Cr2 antibody; CR2_HUMAN antibody; CVID7 antibody; EBV receptor antibody; EBV-R antibody; Epstein Barr virus receptor antibody; Epstein-Barr virus receptor antibody; EVBR antibody; SLEB9 antibody
宿主：

Mouse
反應種屬：

Human
免疫原：

Synthesized peptide derived from human CD21
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG1, Kappa
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

17D8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU. Participates in B lymphocytes activation.; (Microbial infection) Acts as a receptor for Epstein-Barr virus.
基因功能參考文獻：
1. This study shown the C3d+ microglial clusters were found in chronic but not acute multiple sclerosis. PMID: 27778395
2. This study uncovers the origin of the effect of ionic strength on C3d-CR2 interaction and deepens the understanding of the molecular mechanism of their interaction, which is valuable for the design of vaccines and small molecule inhibitors. PMID: 27154286
3. Binding of PF4/heparin complexes to B cells is mediated through the interaction between complement and complement receptor 2. PMID: 27412887
4. high pERK expression and accelerated apoptosis revert within 4 weeks after beginning therapy, whereas clonal B cells unresponsive to TLR9 stimulation persist for at least 24 weeks, although they may partially rescue normal CD21 expression. PMID: 28507081
5. Cerebrospinal fluid (CSF) soluble complement receptor 2 (sCR2) levels correlated significantly both with CSF complements C3 and C1q as well as to a disease severity measure. PMID: 27085202
6. CR2 contributes to human Osteonecrosis of the femoral head susceptibility in Korean systemic lupus erythematosus patients. PMID: 27446959
7. In this study, we have shown that the CR2 +24T>C polymorphism is not a risk marker for nasopharyngeal carcinoma development in the North region of Portugal. PMID: 26748973
8. In mature B cells, CD21 transcription proceeds from a focused transcriptional start site regulated by a non-consensus TATA box, an initiator element and a downstream promoter element. PMID: 25640655
9. Reduced expression of CD21 on CLL B-cells appears functionally relevant and was associated with poor clinical outcomes. PMID: 26452134
10. These data suggest that rs1876453 in CR2 has long-range effects on gene regulation that decrease susceptibility to systemic lupus erythematosus. PMID: 25180293
11. repression of the CR2/CD21 promoter can occur through one of the E-box motifs via recruitment of RP58. PMID: 25817480
12. found that the protective CR2 genotype is correlated with lower levels CR2 mRNA as well as differences in the ratio of the long and short CR2 isoforms PMID: 25569262
13. The paradoxical enhancement of immune responses by C3d in the absence of CD21 is due to internalization and processing of C3d into peptides that activate autoreactive CD4(+) T-helper cells. PMID: 25385064
14. Higher C3d/C3 ratios were found for current smoker, higher age, age-related macular degeneration phenotype, and the two SNPs in the C3 gene, rs6795735 and rs2230199. PMID: 24675670
15. Authors show that expression of the Epstein-Barr virus B-cell attachment receptor, CD21, in B cells that lack this receptor results in significant changes in gene expression, both before and rapidly following EBV-CD21 interaction. PMID: 24600013
16. Our findings implicate CR2 as a susceptibility gene for nasopharyngeal carcinoma and suggest that enhanced CR2 expression may be involved in the oncogenesis and development of nasopharyngeal carcinoma. PMID: 23612877
17. To eradicate this virus, targeting CD21 on these EBV-infected B cells is hypothesized here. PMID: 23384704
18. Patients with primary Sjogren's Syndrome who display high frequencies of autoreactive and unresponsive CD21-/low B cells are susceptible to developing lymphoproliferation. PMID: 23279883
19. plasma sCD21 levels are higher, while B-cell surface CD21 expression levels are lower in B-cell chronic lymphocytic leukemia (B-CLL) patients, but not in multiple myeloma (MM) patients. PMID: 22899340
20. Transgenic mice inoculated with elk brain homogenate expressing high levels of prion (PrPC) protein die of chronic wasting disease. PMID: 23002439
21. All subtypes of juvenile arthritis have reduced circulating levels of sCD21 and sCD23. PMID: 21328056
22. These results suggest that CR2 plays a role in the recognition of foreign DNA during host-immune responses PMID: 22885687
23. the 5' UTR is a novel regulatory element for the CR2 gene in which variation may alter gene function and modify the development of Systemic lupus erythematosus PMID: 22673213
24. Prevalence of pulmonary fibrosis in patients with diffuse cutaneous systemic sclerosis inversely correlates with soluble cluster of differentiation 21 (sCD21) levels. PMID: 21052675
25. Marginal zone B cells activated by hepatitis C virus undergo functional exhaustion associated with BCR signaling defects and overexpression of a key antiproliferative gene, and may subsequently become terminally spent CD21(low) B cells. PMID: 22678901
26. CD21 expression is altered in multiple sclerosis (MS) patients as in other autoimmune diseases, although no evidence is found for a specific role of the CD21 gene in MS. PMID: 22137275
27. Patients with hepatitis C virus infection who display high frequencies of unresponsive CD21(-/low) marginal zone B cells are more susceptible to developing autoimmunity and/or lymphoproliferation. PMID: 22084433
28. CD23 oligomers appear more important in enhancing IgE synthesis and monomers lacking the tail residues fail to bind CD21 yet bind membrane IgE and down-regulate IgE synthesis. PMID: 21889131
29. study reports a cocrystal structure of a CR2(SCR1-2):C3d complex at 3.2 angstrom resolution in which the interaction interfaces differ markedly from the previously published structure and are consistent with the biochemical data PMID: 21527715
30. The impact of hCR2 expression on mouse B cell development and the baseline immunological status of the mice, was evaluated. PMID: 21269698
31. Binding between Epstein Barr virus-transformed B cell line-derived exosomes and viral structural protein gp350 is dependent on CD21 expressed on the B cells. PMID: 21106852
32. Molecular modeling approaches used to investigate disparities between the biochemical data and the X-ray structure of the CR2-C3d cocrystal result in highest-scoring solutions in which CR2 SCR1-2 is docked within the concave surface of C3d. PMID: 20951140
33. Biophysical investigations of complement receptor 2 (CD21 and CR2)-ligand interactions reveal amino acid contacts unique to each receptor-ligand pair PMID: 20558730
34. analysis of the physicochemical properties underlying C3d-CR2 association PMID: 20091675
35. Staphylococcus aureus binder of Ig (Sbi) and complement receptor 2 (CR2) share overlapping binding sites on complement 3d (C3d), thus allowing the bacterial evasion molecule to block CR2 binding, thereby inhibiting the host's adaptive immune response. PMID: 20083651
36. CR2-induced complement activation results in membrane depolarization, as indicated by annexin V binding, with kinetics similar to those of C3-fragment deposition and different from those of membrane attack complex formation. PMID: 19740327
37. promoter activity is critically dependent on a cell type-specific repressor PMID: 11739509
38. Redox control of EBV infection: prevention by thiol-dependent modulation of functional CD21/EBV receptor expression. PMID: 11813981
39. crystal structure of human CD21; implications for epstein-barr virus and c3d binding PMID: 12122212
40. The percentage of T cells expressing CD21 from all gestations was significantly higher than the adult and the percentage positive decreased with increasing gestational age. PMID: 12149502
41. NF-kappaB signaling pathway enhances the expression of the CR2 gene, as a result of NF-kappaB proteins binding to two CR2 promoter elements. PMID: 12444129
42. CR2 is shed mainly as a soluble CR2 complex, in which the CR2 units link covalently and react with nucleophilic agents PMID: 12713795
43. Complement receptor 2-mediated targeting of complement inhibitors to sites of complement activation. PMID: 12813023
44. Coligation of CD21 with the B cell antigen receptor lowers the antigen concentration threshold for eliciting protection from Fas apoptosis by 2-3 orders of magnitude. PMID: 14607925
45. electrostatic calculations provide global and site-specific explanations of the physical causes that underlie the ionic strength dependence of C3d-CR2 association PMID: 15187133
46. The activation of MAPKs is independently mediated by CR2 and that anti-CR2 McAb, PD98059, and Wortmanin block the activation of MAPKs, respectively. PMID: 15603708
47. characterized the interaction between the first two short consensus repeats (SCR1-2) of complement receptor type 2 (CR2, CD21) and C3d PMID: 15713467
48. the open V-shaped structures formed by CR2 SCR 1-2, both when free and when bound to C3d, are optimal for the formation of a tight two-domain interaction with its ligand C3d PMID: 15713468
49. CD21 and CD23a are common targets for B lymphotropic gammaherpesviruses. PMID: 15795251
50. Unresponsiveness to T-dependent antigen (sheep red blood cells) displayed by transgenic CR2-positive B cells is linked to increase in the stimulus level required to propel the B cell into a fully activated state, a normal humoral immune response. PMID: 15905540
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相關疾?。?/div>
Systemic lupus erythematosus 9 (SLEB9); Immunodeficiency, common variable, 7 (CVID7)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein.
蛋白家族：

Receptors of complement activation (RCA) family
組織特異性：

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
數(shù)據(jù)庫鏈接：

HGNC: 2336

OMIM: 120650

KEGG: hsa:1380

UniGene: Hs.445757

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