CLDN7 Monoclonal Antibody

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說明書

中文名稱：

CLDN7鼠單克隆抗體
貨號：

CSB-MA963136
規格：

￥1320
圖片：
IHC image of CSB-MA963136 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CLDN7 Monoclonal Antibody（CUSABIO貨號：CSB-MA963136）是針對人源CLDN7蛋白研發的小鼠單克隆抗體，采用IgG1亞型，適用于ELISA和免疫組化（IHC）等科研檢測。CLDN7屬于緊密連接蛋白家族成員，參與上皮細胞間屏障結構的形成，調控細胞極性及物質選擇性通透，其表達異常與乳腺癌、前列腺癌等腫瘤的發生發展密切相關。本產品以高純度多肽為免疫原制備，經過嚴格質控驗證，具有高特異性和穩定性，可精準識別天然及重組形式的人源CLDN7蛋白，適用于腫瘤微環境研究、上皮細胞功能分析及疾病機制探索等科研場景。通過優化實驗體系，該抗體在石蠟包埋組織切片及蛋白水平檢測中展現優異性能，為癌癥生物標志物篩選和分子病理研究提供可靠工具。關鍵詞：CLDN7單克隆抗體、人源ELISA檢測、免疫組化應用、腫瘤研究、緊密連接蛋白。
產品名稱：

Mouse anti-Homo sapiens (Human) CLDN7 Monoclonal antibody
Uniprot No.：

O95471
基因名：

CLDN7
別名：

CLDN7; CEPTRL2; CPETRL2; Claudin-7; CLDN-7
宿主：

Mouse
反應種屬：

Human
免疫原：

Synthesized peptide derived from human Claudin 7
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

IgG2a, Kappa
純化方式：

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
克隆號：

14F8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a major role in tight junction-specific obliteration of the intercellular space.
基因功能參考文獻：
1. this paper shows that TGF-beta1 alters esophageal epithelial barrier function by attenuation of claudin-7 in eosinophilic esophagitis PMID: 28832026
2. In addition to replicating a previously identified genome-wide significant locus for corneal astigmatism near the PDGFRA gene, gene-based analysis identified three novel candidate genes, CLDN7, ACP2, and TNFAIP8L3, that warrant further investigation to understand their role in the pathogenesis of corneal astigmatism. (Meta-analysis) PMID: 29422769
3. the present study revealed the distinct expression profiles of claudin5, 7 and 8 in nonneoplastic mucosal tissues and gastric carcinoma tissues. Furthermore, the expression of these claudin proteins was highly associated with metastatic progression and prognosis in patients with gastric carcinoma PMID: 29901188
4. DICFM approach may be applied as an appropriate approach to quantify the immunohistochemical staining of claudin-7 on the cell membrane and claudin-7 may serve as a marker for identification of lung cancer PMID: 29512497
5. Cycling hypoxia could induce significant changes in CLDN1 and CLDN7 expression in nasopharyngeal cancer cells, indirectly regulation P18 expression and affecting cell invasion/proliferation. PMID: 28055967
6. These results identify EpCAM as a substrate of matriptase and link HAI-2, matriptase, EpCAM, and claudin-7 in a functionally important pathway that causes disease when it is dysregulated. PMID: 28094766
7. 84/118, 64/118, 52/118 reaction with claudin-1, claudin-3 and claudin-4 in cancer and colon polyps had a membrane localization, respectively.Mislocalization claudin-3 to nucleus in colon cancer and mislocalization claudin-4 to nucleus in adenomas of the colon were detected for the first time. PMID: 28295005
8. suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700
9. that the dysregulated expression of these miRNAs, in conjunction with the high claudin 1 levels, could serve as a useful biomarker that identifies a subset of tumors within the poorly characterized basal-like subtype of breast cancer PMID: 26982264
10. The expression of claudin-7 has no obviously difference between cervical carcinoma tissues and adjacent non-neoplastic tissues. PMID: 26464708
11. We identified hepatocyte nuclear factor 4alpha as a regulatory factor that bound endogenous CLDN7 promoter in differentiating intestinal epithelial cells and stimulated CLDN7 promoter activity. PMID: 26216285
12. we identified a previously unrecognized function of claudin-7 in regulating cell proliferation and maintaining epithelial cell attachment through engaging integrin beta1. PMID: 26081244
13. A variant, rs222857, in the CLDN7 locus, predicted adiponectin increases within intensive lifestyle intervention. PMID: 25759378
14. CLDN-7 palmitoylation prohibits tight junction integration and fosters glycolipid-enriched membrane domains recruitment. Via associated molecules, palmitoylated CLDN-7supports motility and invasion. PMID: 26054340
15. The increased claudin-1 expression was significantly associated with the high pathologic grade, the presence of microscopic perineural invasion, vascular invasion, nodal metastasis, and advanced clinical stage of oral squamous cell carcinoma. PMID: 25078758
16. Data indicate that claudin-7 knockdown cells display decreased anchorage-independent growth. PMID: 25514462
17. Loss of claudin-7 potentiates epithelial-to-mesenchymal transition to promote colon cancer, in a manner dependent on Rab25. PMID: 25500541
18. Icreased claudin 7 expression was related to decreased survival in nasopharyngeal carcinoma. PMID: 25778318
19. Increased expression of claudin-7 is associated with liver cirrhosis and hepatocellular carcinoma. PMID: 24696415
20. This study suggests the possibility that EpCAM, together with CD44v6 and claudin-7 as well as ALDH1, may be involved in the development of the aggressive phenotype of anaplastic thyroid carcinoma. PMID: 24727741
21. these results confirm the role of claudin-1 as a promoter of colon tumorigenesis and further identify the role of the dysregulated antigen-tumor interaction and inflammation in claudin-1-dependent upregulation of colon tumorigenesis. PMID: 24997475
22. Evaluated expression of claudins in gastric cancer and determined their significance for patient outcome. Claudin-3 and claudin-7 were expressed in 25.4% and 29.9% of gastric cancer tissues; 51.5% of gastric cancer tissues had reduced claudin-18. PMID: 24333468
23. These results suggest aberrant Claudin 7, alpha - and beta-catenin expression and/or localisation patterns may be putative markers for distinguishing localised prostate cancer from aggressive metastatic disease when used collectively. PMID: 24358122
24. the CLDN7 rs4562 (c.590C>T) genotype had a higher risk of lymph node involvement and a lower degree of tumor differentiation PMID: 24479816
25. lack of claudin-7 expression in the tumour centre may be used to identify patients with increased risk for regional recurrence PMID: 23953778
26. Claudin-7 was phosphorylated at serine 204 by protein kinase C. PMID: 23433123
27. Survival analysis showed a trend toward a better prognosis among patients with overexpression of claudin-7 in hepatocellular carcinoma. PMID: 23146509
28. Increased expression of claudin-1 contributes to an anti-apoptotic role in TNF-alpha-induced apoptosis. PMID: 22941467
29. CD24+ (P=0.07) and claudin-7 positivity (P=0.05) were associated with reduced time of recurrence, suggesting a contribution of these markers to aggressiveness of breast cancer. PMID: 21956537
30. Claudin-7 and tricellulin were markedly reduced at all stages of tumor development. In situ hybridization analysis showed no correlation between HPV infection and altered expression of the tight junction proteins. PMID: 21480761
31. Claudin-7 is significantly upregulated in epithelial ovarian cancer. PMID: 21789222
32. down-regulation of Claudin-7 and overexpression of Slug in lung squamous cell carcinoma and adenocarcinoma PMID: 21645451
33. The claudin-7 inhibits cell migration and invasion through ERK/MAPK signaling pathway in response to growth factor stimulation in human lung cancer cells. PMID: 21641901
34. transcriptional activity of claudin 7 gene not a useful marker of laryngeal tumor PMID: 21193919
35. Claudin-7 mRNA level is decreased already as an early event in colorectal carcinogenesis, probably contributing to the compromised epithelial barrier in adenomas. PMID: 21310043
36. Claudin-7 down-regulation is an important feature in oral squamous cell carcinoma PMID: 21083599
37. These data suggest that proteasomes regulate claudin-1 localization at the plasma membrane, which changes upon proteasomal inhibition to a Rab5a-mediated endosomal localization. PMID: 20926780
38. Increased expression of claudin-6, claudin-7, or claudin-9 is sufficient to enhance tumorigenic properties of a gastric adenocarcinoma cell line. PMID: 20874001
39. Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis. PMID: 19960275
40. Claudin-1 was expressed in all 18 cases of Epstein-Barr virus-associated nasopharyngeal carcinoma studied PMID: 20204275
41. Loss of claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast, providing insight into the potential role of CLDN-7 in the progression and ability of breast cancer cells to disseminate. PMID: 12673207
42. 2 forms of claudin-7, a full-length form with 211 AA residues and a C-terminal truncated form with 158 AA residues, are able to regulate the expression of a tissue-specific protein, prostate-specific antigen, in the LNCaP prostate cancer cell line. PMID: 14502431
43. Loss of claudin-1 expression proved to be a strong predictor of disease recurrence and poor patient survival in stage II colon cancer. PMID: 15475928
44. Claudin-1 and claudin-7 may play a significant role in tumor progression of cervical neoplasia and may represent useful markers for malignant transformation of cervical squamous cells. PMID: 15790437
45. Overexpression of claudin-7 is associated with gastric tumorigenesis PMID: 16049341
46. induction of claudin7 expression by ELF3 appears critical to the formation of the epithelial structures in biphasic synovial sarcoma PMID: 17060315
47. When compared, adenocarcinomas and squamous cell carcinomas revealed significant differences in CLDN7 expression. PMID: 17418912
48. claudin-7-associated EpCAM is recruited into (tetraspanin-enriched membrane microdomains) and forms a complex with CO-029 and CD44v6 that facilitates metastasis formation PMID: 17579117
49. Results show show that claudin 7 expression changed with the gastric carcinogenic process and that this is implicated in cancer characteristics. PMID: 17611659
50. Claudin-7 to be a candidate expression marker for distinguishing chromophobe renal cell carcinoma from other renal tumor subtypes, including the morphologically similar oncocytoma. PMID: 17922590
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. Cell junction, tight junction.
蛋白家族：

Claudin family
組織特異性：

Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (L
數據庫鏈接：

HGNC: 2049

OMIM: 609131

KEGG: hsa:1366

STRING: 9606.ENSP00000353475

UniGene: Hs.513915

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