CDX2 Monoclonal Antibody

說明書

中文名稱：

CDX2鼠單克隆抗體
貨號：

CSB-MA000214
規格：

￥1090
圖片：
IHC staining of human rectal cancer tissue, diluted at 1:200.

Western blot analysis of 1) 293T, 2) Mouse Heart tissue, diluted at 1:2000.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CDX2單克隆抗體（CUSABIO貨號：CSB-MA000214）是針對腸道特異性轉錄因子CDX2研發的科研用免疫試劑，該靶標蛋白在胚胎腸道發育、腸上皮細胞分化及消化道腫瘤發生中起關鍵調控作用，尤其在結直腸癌研究中常作為特異性生物標志物。本產品采用雜交瘤技術制備，具有高特異性和批次穩定性，經嚴格驗證可同時識別人、小鼠、大鼠等多種哺乳動物樣本中天然與重組CDX2蛋白。在實驗應用場景中，該抗體支持Western Blot檢測（55kDa靶蛋白）、免疫組化（石蠟切片/冰凍切片定位分析）及ELISA檢測（定量分析蛋白表達水平），適用于腸道發育機制研究、消化道腫瘤分子病理分析、轉基因動物模型驗證等科研領域。其廣譜物種兼容性可滿足多維度實驗設計需求，為研究CDX2在腸上皮屏障功能、腫瘤轉移調控及干細胞分化中的分子機制提供可靠工具，是結直腸癌基礎研究與生物標志物篩選的理想選擇，特別推薦用于腸道類器官培養、腫瘤微環境分析等前沿課題的蛋白質水平檢測。
Uniprot No.：

Q99626
基因名：

CDX2
別名：

Caudal type homeo box 2 antibody; Caudal type homeo box transcription factor 2 antibody; Caudal type homeobox 2 antibody; Caudal type homeobox protein 2 antibody; Caudal type homeobox transcription factor 2 antibody; Caudal-type homeobox protein 2 antibody; CDX 2 antibody; CDX 3 antibody; CDX-3 antibody; Cdx2 antibody; CDX2/AS antibody; CDX2_HUMAN antibody; CDX3 antibody; Homeobox protein CDX 2 antibody; Homeobox protein CDX-2 antibody
宿主：

Mouse
反應種屬：

Human,Mouse,Rat
免疫原：

Synthetic Peptide
免疫原種屬：

H, M, R
標記方式：

Non-conjugated
抗體亞型：

IgG
克隆號：

23D10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium. Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription. Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription. Binds to and activates transcription of LPH. Activates transcription of CLDN2 and intestinal mucin MUC2. Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA.
基因功能參考文獻：
1. Sbno1 knockout embryos die at the preimplantation stage without forming a blastocoel, and Cdx2 is not turned on even though both Yap and Tead4 reside normally in nuclei. PMID: 28401892
2. LEF-1 and CDX2 performed at least as well as beta-catenin, if not better, as a diagnostic marker for pilomatrical carcinomas PMID: 29369390
3. Study indicates that CDX2 expression can predict lack of response to NAT. PMID: 29556617
4. CDX2 is not an independent prognostic biomarker in colorectal cancer PMID: 29675807
5. This study characterized a new and original variant of the CDX2 homeobox gene. PMID: 28862703
6. CDX2 is highly expressed along the atrophic gastritis-metaplasia-dysplasia-cancer sequential. Though CDX2 expression is quite dominant in IM ( intestinal metaplasia), but its expression is not associated with H. pylori (Helicobacter pylori) infection. PMID: 29132476
7. of CDX2 using a hypoxia-inducible hTERT promoter-driven vector suppressed malignant progression of colon cancer cells in vitro and in vivo.These results suggest that pLVX-5HRE-hTERTp-CDX2-3FLAG gene therapy may be a promising novel approach to treat colon cancer PMID: 28627695
8. The results of the present study show that a lack of CDX2 expression in metastatic colorectal cancer is an adverse prognostic feature and a potential negative predictor of the response to chemotherapy. PMID: 27726953
9. We may conclude that hTERT promotes GIM by up-regulating CDX2 via NF-kappaB signaling pathway. PMID: 28460480
10. Our work suggests that combination of the consensual CMS classification and lack of CDX2 expression could be a useful marker to identify CMS4/CDX2-negative patients with a very poor prognosis PMID: 28328000
11. AMPK enhances intestinal barrier function and epithelial differentiation via promoting CDX2 expression, which is partially mediated by altered histone modifications in the Cdx2 promoter. PMID: 28234358
12. Findings suggest that H. pylori infectionis a driving force of gastric cardia carcinogenesis by upregulating caudal-related homeobox transcription factor 2 (CDX2) and inducing inflammation. PMID: 27384681
13. Data show that a two-marker panel of p40 (DeltaNp63) and CDX2 is highly sensitive and specific. PMID: 29187465
14. our findings demonstrated that MDR1 expression is regulated by CDX2 and is related to drug resistance in ovarian mucinous adenocarcinoma. PMID: 27060927
15. The data presented provide a novel insight into how CDX2 is implicated in the transcriptional regulation of LAMC2 in intestinal epithelial cells, a function that is impaired during mucosal inflammation where a high level of TNF-alpha is present. PMID: 27333824
16. Cytapheresis might induce CDX2 expression without affecting the cell proliferation, thus resulting in mucosal healing with goblet cell restoration. PMID: 27862316
17. SOX2 expression induces a cellular stem cell state in human colorectal cancer (CRC) with a decreased expression of CDX2. Furthermore, a down-regulated expression of CDX2 results in a poor patient prognosis in CRC. PMID: 27411517
18. A small subset (5%) of hepatocellular carcinomas are CDX2 positive. PMID: 28089540
19. A new organ culture system for adult murine oesophagus is described. Using this, Cdx2 and HNF4alpha were ectopically expressed by adenoviral infection. We demonstrate the expression of CDX2 and HNF4alpha in human biopsy samples. PMID: 27875772
20. Colon cancer cells expressing CDX2 are less deformable, a feature that has been shown to correlate with poor metastatic potential. PMID: 27816490
21. Results show that CDX2 and Fbw7 expression is inversely correlated during colon cell differentiation and that CDX2 expression is regulated by Fbw7 in a GSK3b-dependent manner. PMID: 27470268
22. Aberrant DNA methylation of SOX2 and CDX2 genes contributes to the development of IM. Notably, SOX2 may play a role in establishing and maintaining the methylation status of the CDX2 gene in gastric tissues and cells. PMID: 27913994
23. Strong expression of CDX2 was demonstrated only in the mature colonic epithelium of teratomas in both primary and metastatic germ cell tumors of the testis. PMID: 28322096
24. Cdx members interact with the SWI-SNF complex and make direct contact with Brg1, a catalytic member of SWI-SNF. Both Cdx2 and Brg1 co-occupy a number of Cdx target genes, and both factors are necessary for transcriptional regulation of such targets. Finally, Cdx2 and Brg1 occupancy occurs coincident with chromatin remodeling at some of these loci. PMID: 28082674
25. we found that the loss of CDX2 expression in colorectal cancer is inversely related with the methylation of CDX2 promoter. The methylation of the CDX2 promoter was associated with enhanced lymph node metastases and shorter survival time ( p = 0.0273), but was independent of tumor stage, tumor differentiation, gender, and age. PMID: 27754705
26. PAX2, PAX8, CDX2 immunostains was preformed to the TMA slides. PMID: 26797858
27. CDX2 expression correlates with gastric and intestinal differentiation in Barrett's esophagus towards adenocarcinoma. PMID: 27766003
28. CDX2 inhibited pancreatic adenocarcinoma cell proliferation via promoting tumor suppressor miR-615-5p. Our findings suggested a potential molecular target for pancreatic adenocarcinoma therapy. PMID: 26269116
29. Suggest that NKX6.3 might function as a master regulator of gastric differentiation by affecting SOX2 and CDX2 expression and the NKX6.3 inactivation may result in intestinal metaplasia in gastric epithelial cells. PMID: 26743476
30. Immunohistochemical panel of CDX2, p120ctn, c-Myc, and Jagged1 proteins would be to distinguish between low/high grade dysplasia in histologically challenging cases of Barrett's esophagus. PMID: 26926447
31. CDX2 single-nucleotide polymorphism is associated with cancer risk. PMID: 25738688
32. CK7, TTF-1 and napsin A are predominantly expressed in primary lung adenocarcinoma patients, with CDX-2 being inconsistently expressed. PMID: 26469326
33. Results point out the complex interplay between the DSB DNA repair activity and the homeoproteins HoxB7 and Cdx2 in colon cancer cells. PMID: 26902420
34. findings illuminated a role for NOD1 signaling in attenuating H. pylori-induced Cdx2 expression in gastric epithelial cells PMID: 26759244
35. Nuclear transcription factor CDX2 inhibits gastric cancercell growth and reverses epithelialtomesenchymal transition in vitro and in vivo. PMID: 26238762
36. that CDX2 downregulation is associated with mismatch repair deficiency, right-sided tumors, and poor differentiation at both the mRNA and protein level PMID: 26551082
37. We conclude that CDX2 is a sensitive and relatively specific marker for yolk sac tumor among the nonteratomatous germ cell testicular tumors PMID: 26772394
38. CDX2 expression inhibits the growth of colorectal tumor cells, possibly by downregulating the gene expression. PMID: 26005051
39. Lack of CDX2 expression identified a subgroup of patients with high-risk stage II colon cancer who appeared to benefit from adjuvant chemotherapy. PMID: 26789870
40. Results show an association between Cdx2 polymorphism and an aggressive phenotype of breast neoplasm. PMID: 25849303
41. In active ulcerative colitis HGFR/CDX2 participate in mucosal regeneration by undergoing mesenchymal-to-epithelial transition. PMID: 26229399
42. A non-significant association was observed between VDR cdx2 polymorphism and breast cancer, however the GG genotype was at risk of developing breast cancer. PMID: 25799416
43. Daily intake of vitamin D-FD for 12 weeks improved the central obesity indices in T2D subjects, and the improvement was more pronounced in the carriers of the AA genotype of VDR-Cdx-2. PMID: 26346470
44. Overexpression of CDX2 inhibits the growth of the human gastric cancer cells in vivo and in vitro. PMID: 25738600
45. CDX2 has a better positive predictive value than alcian blue in detecting esophageal intestinal metaplasia. PMID: 25759548
46. Loss of CDX2 expression is associated with aggressive clinical behavior and can be used as a prognostic marker in colorectal cancer. PMID: 25663765
47. CDX2 is acquired de novo in Iintestinal metaplasia and maintained in dysplasia PMID: 25196071
48. CDX2 transcriptionally regulates ST6GalNAc-I gene expression, specifically in the preneoplastic intestinal metaplasia lesion. PMID: 25867765
49. A pancreaticobiliary histomolecular classification (PB) was defined as a sample with pancreaticobiliary histology, positive MUC1 and negative CDX2 expression PMID: 25989273
50. SOX2 combined with CDX2 expression profile in gastric cancer segregate patients into different prognostic groups. PMID: 25300947
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亞細胞定位：

Nucleus.
蛋白家族：

Caudal homeobox family
組織特異性：

Detected in small intestine, colon and pancreas.
數據庫鏈接：

HGNC: 1806

OMIM: 600297

KEGG: hsa:1045

STRING: 9606.ENSP00000370408

UniGene: Hs.127383

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