Recombinant Human Cyclin-dependent kinase 2 (CDK2)

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中文名稱：

Recombinant Human Cyclin-dependent kinase 2(CDK2)
品名簡(jiǎn)稱：

Recombinant Human CDK2 protein
貨號(hào)：

CSB-EP005061HU
說(shuō)明書：

MSDS Datasheet
規(guī)格：

￥1344
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

CDK2
Uniprot No.：

P24941
別名：

Cdc2 related protein kinase; cdc2-related protein kinase; CDC28; CDC2A; Cdk 2; CDK1; CDK2; CDK2_HUMAN; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2)
種屬：

Homo sapiens (Human)
蛋白長(zhǎng)度：

Full Length
來(lái)源：

E.coli
分子量：

37.9kDa
表達(dá)區(qū)域：

1-298aa
氨基酸序列

MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

To make this Recombinant Human CDK2 protein, the CDK2 gene was isolated at first and cloned into an expression vector. CUSABIO has built a mature recombinant protein platform. This Recombinant Human CDK2 protein was developed in the platform. It was expressed in E.coli at the region of 1-298aa of the Human CDK2 protein. N-terminal 6xHis tag was fused with the expression vector for affinity and purification purposes. The purity is 90%+ determined by SDS-PAGE.
Cyclin-dependent kinase 2 (CDK2) is a member of the larger cell cycle regulating CDK family of kinases, activated by binding partner cyclins as its name suggests. In mitotic cells, CDK2 is activated by phosphorylation and binds to E-type cyclins to progress from G1 into S phase, then subsequently binds A-type cyclins in S phase to advance the cell cycle. In particular, it was thought that CDK2/cyclin E complexes were necessary for the phosphorylation of retinoblastoma (RB) protein and the release of E2F transcription factors to initiate S phase and DNA synthesis. Further analysis reveals that the CDK2 substrate RB remains phosphorylated, even at sites previously thought to be specific to CDK2, suggesting a compensatory mechanism by another kinase. During the last two decades, CDK2 inhibitors have not been developed for contraception but instead predominantly to treat cancer. Multiple CDK2 inhibitors have entered clinical trials, none have been approved by the Food and Drug Administration.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1. Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability. Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.
基因功能參考文獻(xiàn)：
1. Results indicate that although PIN1 increases p27 levels, it also attenuates p27's inhibitory activity on CDK2 and thereby contributes to increased G1-S phase transitions and cell proliferation. PMID: 29118189
2. CDK2 mutation is not associated with non-obstructive azoospermia. PMID: 29373224
3. Proteomics and phosphoproteomics analyses identified CDK2 as a driver of resistance to both BRAF and Hsp90 inhibitors and its expression is regulated by the transcription factor MITF upon XL888 treatment of melanoma cells. PMID: 29507054
4. identified a new phosphorylation-based substrate recognition mechanism of PTPN12 by CDK2, which orchestrated signaling crosstalk between the oncogenic CDK2 and HER2 pathways PMID: 28842430
5. CDK2 gene is a strong candidate gene for type-2 diabetes. CDK2 gene is located in a risk area composed of 4 blocks in strong LD around the type-2 diabetes SNP rs2069408. CDK2 overexpression inhibits the association of insulin receptor to the microtubule components, tubulin alpha and tubulin beta. Physical association of the insulin receptor complex with CDK2 is inhibited by the expression of tyrosine phosphatase PTPLAD1. PMID: 30300385
6. Among these genes, STAT3 and CDK2 were significantly associated with recurrence. Further study suggested that inhibition of CDK2 reduced invasion of Pca cell lines. The invasion ability was rescued after reintroduction of CDK2. PMID: 29323532
7. The roles of the CDK2/SIRT5 axis in gastric cancer. PMID: 29896817
8. CDK2 may have key functions in neuroblastoma progression by regulating the expression of neoplastic genes. PMID: 29328425
9. The authors show that human Cyclin-Dependent-Kinases (CDKs) target the RAD9 subunit of the 9-1-1 checkpoint clamp on Thr292, to modulate DNA damage checkpoint activation. Thr292 phosphorylation on RAD9 creates a binding site for Polo-Like-Kinase1 (PLK1), which phosphorylates RAD9 on Thr313. PMID: 29254517
10. this study suggests that CDK2 and CDK9 are potential therapeutic targets in Neuroblastoma (NB) and that abrogating CDK2 and CDK9 activity by small molecules like dinaciclib is a promising strategy and a treatment option for NB patients PMID: 27378523
11. LINC00958 acts as an oncogenic gene in the gliomagenesis through miR-203-CDK2 regulation, providing a novel insight into glioma tumorigenesis. PMID: 29570358
12. These compounds bind CDK2/ Cyclin A, inhibit its kinase activity, compete with substrate binding, but not with ATP, and dock onto the T-loop of CDK2. The best compound also binds CDK4 and CDK4/Cyclin D1, but not CDK1. PMID: 28430399
13. CDK2 contributes to S81-AR phosphorylation and transactivation while CDK4 was not shown to be involved in this process. PMID: 29157894
14. Our findings provide a rationale for clinical use of Bcl-2 family inhibitors in combination with CDK2 inhibitors for treatment of Mcl-1-dependent colorectal tumours associated with expression of Bcl-2, Bcl-XL and Bcl-w proteins. In addition, we have shown potential of CDK2 inhibitors for treatment of tumours expressing R273H mutant p53. PMID: 29372687
15. CDK2 phosphorylates polyQ-AR specifically at Ser(96). Phosphorylation of polyQ-AR by CDK2 increased protein stabilization and toxicity and is negatively regulated by the adenylyl cyclase/protein kinase A signaling pathway in spinobulbar muscular atrophy. PMID: 28003546
16. Data show that Noxa-mediated MCL-1 phosphorylation and degradation is regulated by CDK2. PMID: 27166195
17. The upregulation of miR-302b reduced the expression of CDK2, and inhibited ERK signaling pathway, thereby inhibiting cell proliferation and G1/S phase conversion rate. PMID: 27465546
18. High CDK2 expression is associated with breast cancer. PMID: 28760857
19. Here, we introduce a transcriptional signature to specifically track CDK2 activity. It responds to genetic and chemical perturbations in the CDK-RB-E2F axis, correlates with mitotic rate in vitro and in vivo and reacts rapidly to changes in CDK2 activity during cell cycle progression PMID: 27819669
20. Here, we found that centrosomal protein of 76 kDa (Cep76), previously shown to restrain centriole amplification, interacts with cyclin-dependent kinase 2 (CDK2) and is a bona fide substrate of this kinase. Cep76 is preferentially phosphorylated by cyclin A/CDK2 at a single site S83, and this event is crucial to suppress centriole amplification in S phase PMID: 27065328
21. The authors find that Spy1 confers structural changes to Cdk2 that obviate the requirement of Cdk activation loop phosphorylation. PMID: 28666995
22. CDK2 serves as an important nexus linking primary beta-cell dysfunction to progressive beta-cell mass deterioration in diabetes PMID: 28100774
23. a SUMO-deficient Rb mutant results in reduced SUMOylation and phosphorylation, weakened CDK2 binding, and attenuated E2F-1 sequestration. PMID: 27163259
24. These two states are separated by different metastable states that share hybrid structural features with both forms of the kinase. In contrast, the CDK2/ANS complex landscape is compatible with a conformational selection picture where the binding of ANS in proximity of the alphaC helix causes a population shift toward the inactive conformation PMID: 27100206
25. Dsg2 knockdown arrests non-small cell lung cancer cell cycle progression via modulation of p27-CDK2 levels. PMID: 27629878
26. CDK2 protects podocytes from apoptosis and reduced expression of CDK2 associates with the development of diabetic nephropathy. PMID: 26876672
27. CDK2 controls a wide-spread epigenetic program that drives transcription at differentiation-related gene promoters specifically in G1. (Review) PMID: 26857166
28. SATB2 regulates the mitosis of cell cycle and affects G1 cell cycle via interaction with CDK2. PMID: 26714749
29. A novel link has been discussed between CDK2 expression and cell migration by characterizing the CDK2-mediated phosphorylation of BRMS1. PMID: 26730572
30. the results suggest that CK1delta activity can be modulated by the interplay between CK1delta and CDK2/E or CDK5/p35. PMID: 26464264
31. Inappropriate activation of CDK2 in S phase underlies the sensitivity of a subset of cell lines to Chk1 inhibitors. PMID: 26595527
32. CDK2 and DNA-PK regulate PR transcriptional activity by distinct mechanisms. PMID: 26652902
33. The expression of cdk2 in malignancy of ovarian tumors. PMID: 26828990
34. PHD1 is phosphorylated by CDK2, CDK4 and CDK6 at Serine 130. PMID: 26644182
35. Results show that miR-200c plays an antioncogenic role in clear cell renal cell carcinomas, through controlling cell growth and cell-cycle progression by downregulating the G1-S regulator CDK2. PMID: 26248649
36. show thata combining cyclin-dependent kinase 2 (CDK2) antagonism and ubiquitin thioesterase 33 (USP33) depletion augments anaphase catastrophe via changes in centrosomal protein of 110 kDa (CP110) protein expression. PMID: 26304236
37. Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2. PMID: 26861625
38. our study reveals a novel function of CDK2 in EGF-induced cell transformation and the associated signal transduction pathways. This indicates that CDK2 is a useful molecular target for the chemoprevention and therapy against skin cancer. PMID: 26028036
39. both cell lines feature a significant reduction of CDK2 expression verified at the RNA and protein level, respectively PMID: 26555773
40. Centriolar satellites build a centrosomal microcephaly protein complex critical for human neurodevelopment that promotes CDK2 centrosomal localization and centriole duplication. PMID: 26297806
41. Identified ING5 as a novel CDK2 substrate. ING5 is phosphorylated at a single site, threonine 152, by cyclin E/CDK2 and cyclin A/CDK2. This site is also phosphorylated in cells in a cell cycle dependent manner, consistent with it being a CDK2 substrate. PMID: 25860957
42. fluspirilene is a potential CDK2 inhibitor and a candidate anti-cancer drug for the treatment of human hepatocellular carcinoma. PMID: 26147897
43. analysis of the conformational characteristics and ligand binding mechanisms of CDK2 [review] PMID: 25918937
44. Which is mutated at the CDK2 phosphorylation site. PMID: 25154617
45. observations suggested that androgen suppresses the proliferation of CRPC cells partially through inhibition of Cyclin A, Cdk2, and Skp2 PMID: 25271736
46. A positive correlation between cdk2/cyclin A expression level and tumor growth. Amygdalin, therefore, may block tumor growth. PMID: 25136960
47. Report structure-based discovery of allosteric inhibitors of CDK2. PMID: 24911186
48. Sox2 phosphorylation by Cdk2 promotes the establishment but not the maintenance of the pluripotent state. PMID: 26139602
49. Inhibition of CDK2 phosphorylation blocked phosphorylation of hnRNP K, preventing its incorporation into stress granules (SGs). Due to interaction between hnRNP K with TDP-43, the loss of hnRNP K from SGs prevented accumulation of TDP-43. PMID: 25410660
50. The docking and molecular dynamics investigation performed here led to the identification of the interactions responsible for stabilizing the ligand ChEMBL474807 at the active sites of the glycogen synthase kinase-3beta (GSK-3) and cyclin-dependent kinase-2 PMID: 25754137
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亞細(xì)胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Note=Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).
蛋白家族：

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily
數(shù)據(jù)庫(kù)鏈接：

HGNC: 1771

OMIM: 116953

KEGG: hsa:1017

STRING: 9606.ENSP00000266970

UniGene: Hs.19192