CA9 Recombinant Monoclonal Antibody

說明書

中文名稱：

CA9重組抗體
貨號(hào)：

CSB-RA614990A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA614990A0HU diluted at 1:118 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-RA614990A0HU diluted at 1:118 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA614990A0HU CA9重組單克隆抗體是針對(duì)碳酸酐酶IX（CA9）靶點(diǎn)開發(fā)的高特異性科研試劑。CA9是一種跨膜蛋白，在多種實(shí)體瘤（如腎細(xì)胞癌、宮頸癌）的缺氧微環(huán)境中顯著高表達(dá)，通過調(diào)節(jié)細(xì)胞內(nèi)pH值參與腫瘤細(xì)胞增殖、侵襲及轉(zhuǎn)移等病理過程。本抗體采用重組技術(shù)制備，經(jīng)ELISA和免疫組化（IHC）驗(yàn)證，在石蠟包埋組織切片中展現(xiàn)出優(yōu)異的抗原識(shí)別能力，推薦使用稀釋比例為1:50-1:200，可清晰呈現(xiàn)CA9在腫瘤組織中的定位與表達(dá)水平。該產(chǎn)品適用于癌癥發(fā)生機(jī)制研究、腫瘤微環(huán)境分析以及生物標(biāo)志物探索等領(lǐng)域，特別在腎癌病理模型構(gòu)建、缺氧相關(guān)信號(hào)通路研究及抗腫瘤藥物靶點(diǎn)驗(yàn)證中具有重要應(yīng)用價(jià)值。通過嚴(yán)格的表達(dá)特異性和批次穩(wěn)定性檢測(cè)，本抗體可為體外實(shí)驗(yàn)提供可靠的工具，助力科研人員深入解析CA9在腫瘤生物學(xué)中的功能及調(diào)控網(wǎng)絡(luò)。
Uniprot No.：

Q16790
基因名：

CA9
別名：

CA-IX antibody; CA9 antibody; CAH9_HUMAN antibody; CAIX antibody; Carbonate dehydratase IX antibody; Carbonic anhydrase 9 antibody; Carbonic anhydrase IX antibody; Carbonic dehydratase antibody; G250 antibody; Membrane antigen MN antibody; MN antibody; P54/58N antibody; pMW1 antibody; RCC associated protein G250 antibody; RCC-associated antigen G250 antibody; Renal cell carcinoma-associated antigen G250 antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human CA9
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

4F12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
基因功能參考文獻(xiàn)：
1. Our findings indicated that CA-IX may be a potential prognostic biomarker in the treatment of glioblastoma multiforme PMID: 29952031
2. Our results do not suggest a prognostic role for CA IX overexpression in stage III non-small cell lung cancer patients who received neoadjuvant treatment. PMID: 30029935
3. FZD1 and CAIX might be important biological markers for the carcinogenesis, metastasis, invasion, and prognosis of pancreatic ductal adenocarcinoma PMID: 28921449
4. High expression of CA9 is associated with breast cancer. PMID: 29893327
5. Data suggest that the conformational flexibility may have several important roles in tumour progression, facilitating interactions of carbonic anhydrase IX (hCA IX) with partner proteins assisting tumour spreading and progression. PMID: 29564477
6. the findings of this study establish the SOX9/CA9-mediated oncogenic pathway in glioma, the inhibition of which enhances the sensitivity of glioma cells to Temozolomide (TMZ) treatment, and thus highlights the value of developing small molecules or antibodies against the SOX9/CA9 pathway, for combination therapy with TMZ, in the more efficient management of glioma PMID: 29749469
7. CA9 SNP rs1048638 and haplotype A1AA are associated with the susceptibility of cervical invasive squamous cell carcinoma for Taiwanese women PMID: 29725249
8. The TCGA data revealed that CAIX expression was significantly higher in bladder cancer specimens than in normal tissue. PMID: 29949785
9. CAIX enzyme inhibition assay showed the IC50 values in nM range. Though all the three compounds (1-3) showed a good binding with CAIX, compound 2 showed the best inhibition of CAIX activity. These compounds were non-toxic on normal cell lines (HEK-293) and significantly inhibit the proliferation of hypoxic cancer cells PMID: 28830777
10. CA9 expression was observed in normal islets, while neuroendocrine microadenomas and small (< 1 cm) pancreatic neuroendocrine neoplasms/tumors showed CA9 expression loss. PMID: 29666945
11. Study revealed that CA9 expression was a pivotal predictive factor for poor prognosis after radical surgery for HCC. Moreover, the CA9 regulation of the expression of EMT-related molecules represented a mechanism that enhanced malignant potential. PMID: 28849188
12. Report weak CA9 immunoreactivity in majority of CA9 positive colorectal carcinoma cases associated with macroscopic growth pattern. PMID: 28554753
13. Molecular characterization of carbonic anhydrase IX catalytic domain has been presented. PMID: 27373313
14. Authors present evidence that enhanced autophagy does not play a role in the enhanced growth of the CA9+ cells. Our study suggests a direct in vivo functional link between hypoxic cells and CICs in primary cervix cancer xenografts. PMID: 27901496
15. Genetic disruption of the intracellular pH-regulating proteins Na+/H+ exchanger 1 (SLC9A1) and carbonic anhydrase 9 reduces the proliferation of colon cancer cells. PMID: 28055960
16. Data show that targeting the hypoxic tumor compartment by knockdown of carbonic anhydrase IX (CAIX) using short hairpin RNA or by chemical inhibition of CAIX with acetazolamide potentiates the anti-cancer activity of mTOR inhibitors rapamycin. PMID: 27153561
17. Authors demonstrate that CAIX associates with MMP14 through potential phosphorylation residues within its intracellular domain, and that CAIX enhances MMP14-mediated collagen degradation by directly contributing hydrogen ions required for MMP14 catalytic activity. PMID: 28692057
18. Results provide evidence that CAIX induces chemoresistance of A549 cells, a lung cancer cell line. PMID: 28028936
19. The results suggest the possibility that CA9 exosomes released from hypoxic RCC may enhance angiogenesis in microenvironment, thereby contributing to cancer progression. PMID: 28851650
20. CA9 expression is highly associated with distant metastases, including para-aortic involvement. PMID: 27102843
21. Increased miR-210 and concomitant decreased ISCU RNA levels were found in ~40% of tumors and this was significantly associated with HIF-1alpha and CAIX, but not MCT1 or MCT4, over-expression. PMID: 28099149
22. describe the identification of the structural determinants responsible for the CA IX/CAND1 interaction PMID: 28388044
23. Data suggest that CAIX (carbonic anhydrase IX) is a novel downstream mediator of asTF (alternatively spliced tissue factor) in pancreatic ductal adenocarcinoma, particularly under hypoxic conditions that model late-stage tumor microenvironment; tumor hypoxia appears to lead to up-regulation of CAIX expression (or 'activation'), which is more pronounced in tumor cells overexpressing asTF. PMID: 27721473
24. Our findings suggest that LCN2 suppresses tumour metastasis by targeting the transcriptional and post-transcriptional regulation of CAIX in oral squamous cell carcinoma PMID: 27207653
25. The findings suggest that CA9, and particularly its carbonic anhydrase activity, promotes the tumorigenicity of adult T-cell leukemia/lymphoma-derived cells and may be involved in malignant development of lymphoma-type adult T-cell leukemia/lymphoma. PMID: 28075522
26. We demonstrated that the expression levels of glycolysis-related proteins glucose transporter 1, hexokinase II, carbonic anhydrase IX, and monocarbonylate transporter 4 differ between thyroid cancer subtypes and are correlated with poorer prognosis PMID: 28347233
27. CAIX and PTEN had prognostic importance for metastatic renal cell carcinoma patients receiving first-line VEGFR TKI. Future validation and mechanistic studies are required. PMID: 26526582
28. combined HIF1alpha and CAIX protein expression may serve as an unfavorable prognostic indicator particularly in patients treated with cyclophosphamide-based chemotherapy or radiotherapy as well as those with basal phenotype of breast cancer PMID: 27184798
29. CA9 may have a role in castration-resistant prostate cancer progression PMID: 27630286
30. The diagnostic accuracy of CA9 expression for clear cell renal cell carcinoma was 100%. PMID: 27775441
31. High expression of CA IX was associated with pancreatic cancer. PMID: 26224207
32. CAIX mRNA expression was significantly higher (p < 0.05) in hypoxia for all cell lines, which was in agreement with protein expression by ICC. CAXII expression was mixed, with a modest hypoxia-related increase in two cell lines (p < 0.05) and no change in others. PMID: 26276155
33. We also demonstrated that the cell-surface CA IX level dropped during the death progress due to an increased ECD shedding, which required a functional ADAM17. Inhibitors of metalloproteinases reduced CA IX ECD shedding, but not apoptosis. PMID: 26993100
34. In neuroblastoma cells, CAIX and PGK1 expression is up regulated under hypoxia and correlates with response to targeted anti-proliferative treatment. PMID: 26510737
35. Flow cytometrically sorted CA9+ population showed increased mRNA level of a Wnt signaling factor AXIN2. In conclusion, these observations indicate that CA9 expression in normal crypt base cells has association with intestinal epithelial stemness PMID: 26648507
36. We used cobalt chloride (CoCl2) as a hypoxia-mimetic agent and found that the expression of HIF-1a protein, CA IX mRNA and protein, is effectively upregulated, except for HIF-1a mRNA. PMID: 26648580
37. We conclude that CA9/miR34 interplay shares in the hypoxic regulation of mammospheres and therefore, may play a relevant role in the hypoxic breast cancer stem cell niche. PMID: 26553365
38. Knockdown of CAIX significantly reduced proliferation of cancer cells, suggesting that rapid efflux of lactate and H(+), as enhanced by CAIX, contributes to cancer cell survival under hypoxic conditions. PMID: 26337752
39. Data indicate that carbonic anhydrase IX (CAIX) inhibition as a relevant therapeutic goal in breast cancer, targeting the migratory, invasive, and metastatic potential of this disease. PMID: 26259239
40. Inhibition of CA9 expression or activity resulted in radiation sensitization of RCC in a preclinical mouse model. PMID: 26252502
41. Urinary CAIX has a high sensitivity and specificity for diagnosing urothelial bladder cancer. PMID: 26138037
42. The intrinsic thermodynamic parameters of compound binding to CA IX helped to draw the compound structure to thermodynamics relationship. PMID: 26794023
43. our study showed that the expression of CAIX in oral squamous cell carcinoma (OSCC) samples can predict the progression of OSCC and survival of OSCC patients in Taiwan PMID: 26130414
44. we aimed to determine the effect of immunohistochemical staining of ezrin, carbonic anhydrase IX (CA IX), and neuropilin-2 on the prognosis of patients diagnosed with metastatic RCC who were treated with TKIs between January 2007 and June 2012. PMID: 26026587
45. CA IX is expressed in B-cell lymphomas and is qualitatively correlated with extracellular acidosis in xenograft tumor models. PMID: 25130478
46. We have developed an efficient system for the production of the catalytic domain of CA IX in methylotrophic yeast Pichia pastoris PMID: 26522624
47. CAIX expression is increased in hypoxia to compensate for the decrease in its activity produced by a low extracellular pH. A major function of CAIX is to lower the extracellular pH. PMID: 26249175
48. A strong positive correlation between the mRNA expression levels of HIF-2alpha, CA9, VEGF, GLUT-1 and OPN suggests a specific hypoxia-associated profile of mRNA expression in glioblastoma multiforme PMID: 25963717
49. expression of the CA-IX protein is a crucial predictor of poor prognosis in resectable hepatocellular carcinoma PMID: 25738958
50. Preventing carbonic anhydrase IX association with 45 S rDNA gene promoters. PMID: 25793203
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亞細(xì)胞定位：

Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus.
蛋白家族：

Alpha-carbonic anhydrase family
組織特異性：

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
數(shù)據(jù)庫鏈接：

HGNC: 1383

OMIM: 603179

KEGG: hsa:768

STRING: 9606.ENSP00000367608

UniGene: Hs.63287

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