Mouse Thyroid-Peroxidase,TPO ELISA Kit

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中文名稱：

小鼠甲狀腺過氧化物酶(TPO)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08353m
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
促銷：
其他：

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產(chǎn)品描述：

小鼠甲狀腺過氧化物酶(TPO)酶聯(lián)免疫試劑盒（CSB-E08353m）為競爭法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的TPO含量。TPO（血小板生成素）是刺激巨核細(xì)胞生長及分化的內(nèi)源性細(xì)胞因子。它作用于血小板生成各階段，促進(jìn)巨核祖細(xì)胞增殖和分化，增加血小板生成。其研究機(jī)制聚焦于明確信號(hào)傳導(dǎo)通路等，對(duì)治療血小板減少癥等血液疾病意義重大。試劑盒檢測(cè)范圍為2.5 ng/mL-40 ng/mL，該產(chǎn)品特別適用于甲狀腺功能調(diào)控機(jī)制研究、自身免疫性甲狀腺疾病模型建立、藥物或環(huán)境因素對(duì)甲狀腺代謝影響的評(píng)估等場景，為探索甲狀腺相關(guān)分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
縮寫：

TPO
Uniprot No.：

P35419
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測(cè)范圍：

2.5 ng/mL-40 ng/mL
靈敏度：

2.5 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長：

450 nm
研究領(lǐng)域：

Cancer
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-103
1:2	Average %	89
	Range %	82-97
1:4	Average %	95
	Range %	87-100
1:8	Average %	90
	Range %	83-99

回收率：

The recovery of mouse TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-102
EDTA plasma (n=4)	95	90-103

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.272	0.263	0.268
20	0.525	0.530	0.528
10	0.894	0.874	0.884
5	1.334	1.377	1.356
2.5	2.554	2.528	2.541

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Notch1 regulates hepatic thrombopoietin production Y Sun,Blood,2024
Multi-regulatory potency of USP1 on inflammasome components promotes pyroptosis in thyroid follicular cells and contributes to the progression of Hashimoto's thyroiditis X Zhao, W Ni, W Zheng, W Ni, C Sun, Y Gu, Z Gu,Molecular Medicine,2024
Puerarin Alleviates Experimental Autoimmune Thyroiditis by Regulating Macrophages Q Tao,Journal of immunology,2024
Clinical implications of TPO and AOX1 in pediatric papillary thyroid carcinoma Y Yu,Translational Pediatrics,2021

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功能：

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
基因功能參考文獻(xiàn)：
1. Lnk Deficiency Leads to TPO-Mediated Osteoclastogenesis and Increased Bone Mass Phenotype PMID: 28067429
2. Hearing impairment in hypothyroid dwarf mice caused by mutations of the thyroid peroxidase gene. PMID: 24297261
3. A novel hypothyroid dwarfism is due to the missense mutation Arg479Cys of the thyroid peroxidase gene in the mouse. PMID: 16762971
亞細(xì)胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Peroxidase family, XPO subfamily
數(shù)據(jù)庫鏈接：

KEGG: mmu:22018

STRING: 10090.ENSMUSP00000021005

UniGene: Mm.4991

Mouse Thyroid-Peroxidase,TPO ELISA Kit

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